The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Role of glucose transporters in the cellular insulin resistance of type II non-insulin-dependent diabetes mellitus.
Open accessAbstract
To examine the role of glucose transport proteins in cellular insulin resistance, we studied subcutaneous adipocytes isolated from lean control, obese control (body mass index [BMI] 33.4 +/- 0.9), and untreated obese non-insulin-dependent diabetes mellitus (NIDDM) patients (BMI 35.2 +/- 2.1; fasting glucose 269 +/- 20 mg/dl). Glucose transporters were measured in plasma membrane (PM), low-density (LDM), and high-density (HDM) microsomal subfractions from basal and maximally insulin-stimulated cells using the cytochalasin B binding assay, and normalized per milligram of membrane protein. In all subgroups, insulin led to an increase in PM glucose transporters and a corresponding depletion of transporters in the LDM. Insulin recruited 20% fewer transporters to the PM in the obese subgroup when compared with lean controls, and this was associated with a decline in LDM transporters with enlarging cell size in the control subjects. In NIDDM, PM, and LDM, transporters were decreased 50% in both basal and stimulated cells when compared with obese controls having similar mean adipocyte size. Cellular depletion of glucose transporters was not the only cause of insulin resistance, because the decrease in rates of [14C]-D-glucose transport (basal and insulin-stimulated) was greater than could be explained by reduced numbers of PM transporters in both NIDDM and obesity. In HDM, the number of transporters was not influenced by insulin and was similar in all subgroups. We conclude that (a) in NIDDM and obesity, both reduced numbers and impaired activity of glucose transporters contribute to cellular insulin resistance, and (b) in NIDDM, more profound cellular insulin resistance is associated primarily with a further depletion of cellular transporters.
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“In insulin resistant individuals with T2DM, glucose transport activity is severely impaired [156, 181, 182, 187–189].”
From Pathogenesis of insulin resistance in skeletal muscle (2010) · cites this paper as [187]
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Research works printed but not linked: 1
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Pathogenesis of type 2 diabetes mellitus
DeFronzo, R. A. (2004) · Medical Clinics of North America · doi:10.1016/j.mcna.2004.04.013 · Cited by 1,342
Martin, B. C., Warram, J. H., Krolewski, A. S., Soeldner, J. S., Kahn, C. R., Martin, B. C., & Bergman, R. N. (1992) · The Lancet · doi:10.1016/0140-6736(92)92814-v · Cited by 1,158
Laakso, M., Edelman, S. V., Brechtel, G., & Baron, A. D. (1990) · Journal of Clinical Investigation · doi:10.1172/jci114644 · Cited by 930
Insulin Resistance — Mechanisms, Syndromes, and Implications
Epstein, F. H., Moller, D. E., & Flier, J. S. (1991) · New England Journal of Medicine · doi:10.1056/nejm199109263251307 · Cited by 807
The glucose transporter family: structure, function and tissue-specific expression
Gould, G. W., & Holman, G. D. (1993) · Biochemical Journal · doi:10.1042/bj2950329 · Cited by 785
Metadata
- Authors
- Garvey, W. T., Huecksteadt, T. P., Matthaei, S., & Olefsky, J. M.
- Affiliations
- University of California San Diego (US)
- United States Department of Veterans Affairs (US)
- Venue
- Journal of Clinical Investigation
- Published
- 1988-05-01
- Volume
- 81
- Issue
- 5
- Pages
- 1528-1536
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC442586
- OpenAlex
- W2143122636
- Cited by
- 169
- References
- 42
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 4 · 2017: 1 · 2016: 1 · 2015: 5 · 2014: 2 · 2013: 3
- FWCI
- 6.06
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Pfizer
- Deutsche Forschungsgemeinschaft
- National Institutes of Health (AM-33639, 2r01am033651-03)
- National Center for Research Resources (RR00827)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Keywords
- glucose transporters, insulin resistance, type 2 diabetes, adipocytes, obesity, insulin-stimulated glucose transport, plasma membrane, low density microsomes, adipocyte size
- MeSH terms
- Adipose Tissue, Adult, Blood Glucose, Cell Membrane, Diabetes Mellitus, Diabetes Mellitus, Type 2, Female, Humans, Insulin Resistance, Male, Microsomes, Monosaccharide Transport Proteins, Obesity
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