The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Lack of HLA class I antigen expression by cultured melanoma cells FO-1 due to a defect in B2m gene expression.
Open accessAbstract
The melanoma cell line FO-1 does not express HLA class I antigens and does not acquire them on the cell surface after incubation with IFN-gamma. Immunochemical studies showed that FO-1 cells synthesize HLA class I heavy chain, but do not synthesize beta 2-microglobulin (beta 2-mu). The latter abnormality is associated with lack of beta 2-mu mRNA which remains undetectable in FO-1 cells incubated with IFN-gamma. The defect was identified as a genetic lesion in the B2m gene, since DNA hybridization analysis detected a deletion of the first exon of the 5'-flanking region, and of a segment of the first intron of the B2m gene. HLA class I antigen expression was reconstituted on melanoma cells FO-1 after transfection with the wild-type mouse B2m gene, thereby confirming the abnormality of the endogenous B2m gene. The defect identified in FO-1 cells is distinct from that underlying the lack of HLA class I antigen expression by lymphoblastoid cells Daudi, but is remarkably similar to that causing lack of H-2 class I antigen expression by mouse lymphoblastoid cells R1 (TL-). These results suggest that genetic recombination in the 5' region of the B2m gene is a recurrent mechanism in B2m gene defects. In addition to contributing to our understanding of molecular abnormalities in HLA class I antigen expression by melanoma cells, FO-1 cells represent a useful model for analyzing the role of HLA class I antigens in the biology of melanoma cells and in their interaction with cells of the immune system.
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Self-citation
Metadata
- Authors
- D'Urso, C. M., Wang, Z. G., Cao, Y., Tatake, R., Zeff, R. A., & Ferrone, S.
- Venue
- Journal of Clinical Investigation
- Published
- 1991-01-01
- Volume
- 87
- Issue
- 1
- Pages
- 284-292
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2154298769
- Cited by
- 309
- References
- 46
- FWCI
- 4.59
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Lack of HLA class I antigen expression by cultured melanoma cells FO-1 due to a defect in B2m gene expression. https://app.cochat.ai/papers/10.1172/jci114984
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- D'Urso, C. M., Wang, Z. G., Cao, Y., Tatake, R., Zeff, R. A., & Ferrone, S. (1991). Lack of HLA class I antigen expression by cultured melanoma cells FO-1 due to a defect in B2m gene expression. Journal of Clinical Investigation. https://doi.org/10.1172/jci114984
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