The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Thrombin stimulates proliferation of cultured rat aortic smooth muscle cells by a proteolytically activated receptor.
Open accessAbstract
Thrombin has been implicated in the stimulation of smooth muscle cell (SMC) proliferation that contributes to post angioplasty restenosis. The present studies demonstrated that human alpha-thrombin was a potent and efficacious mitogen for cultured rat aortic SMC, stimulating an increase in 3H-thymidine incorporation, as well as an increase in cell number at 1 to 10 nM concentration. gamma-Thrombin, which is enzymatically active but lacks fibrinogen clotting activity, stimulated SMC mitogenesis but was approximately 10-fold less potent than alpha-thrombin. In contrast, D-phenylalanyl-L-propyl-L-arginyl-chloromethyl ketone-alpha-thrombin, which lacked enzymatic activity, had no mitogenic effect. Diisopropylfluorophosphate-alpha-thrombin failed to stimulate mitogenesis except at concentrations having equivalent enzymatic activity as that of alpha-thrombin at its threshold for mitogenesis. Thus, thrombin-induced proliferation was dependent on enzymatic activity. A 14-residue peptide (SFLLRNPNDKYEPF) corresponding to amino acids 42 through 55 of the human thrombin receptor (Vu, T. K., D. T. Hung, V. I. Wheaton, and S. R. Coughlin, 1991. Cell. 64:1057-1068) had full efficacy in stimulating SMC proliferation. Reversing the first two amino acids of this peptide abolished mitogenic activity. Northern analysis demonstrated that SMC expressed a single mRNA species that hybridized to a labeled thrombin receptor cDNA probe. These findings indicate that alpha-thrombin stimulates SMC proliferation via the proteolytic activation of a receptor very similar or identical to that previously identified.
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Cites
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Self-citationRoss, R., Glomset, J., Kariya, B., & Harker, L. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.4.1207 · Cited by 1,847
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Biochemical and Biological Attributes of Matrix Metalloproteinases
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Structure, function and pathophysiology of protease activated receptors
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Stimulation of a Vascular Smooth Muscle Cell NAD(P)H Oxidase by Thrombin
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Metadata
- Authors
- McNamara, C. A., Sarembock, I. J., Gimple, L. W., Fenton, J. W., Coughlin, S. R., & Owens, G. K.
- Venue
- Journal of Clinical Investigation
- Published
- 1993-01-01
- Volume
- 91
- Issue
- 1
- Pages
- 94-98
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2166170833
- Cited by
- 499
- References
- 22
- FWCI
- 23.64
- Type
- journal article
- Language
- en
- Open access
- bronze
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- McNamara, C. A., Sarembock, I. J., Gimple, L. W., Fenton, J. W., Coughlin, S. R., & Owens, G. K. (1993). Thrombin stimulates proliferation of cultured rat aortic smooth muscle cells by a proteolytically activated receptor. Journal of Clinical Investigation. https://doi.org/10.1172/jci116206
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
