The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Recombinant peanut allergen Ara h I expression and IgE binding in patients with peanut hypersensitivity.
Open accessAbstract
Peanut allergy is a significant health problem because of the frequency, the potential severity, and the chronicity of the allergic sensitivity. Serum IgE from patients with documented peanut hypersensitivity reactions and a peanut cDNA expression library were used to identify clones that encode peanut allergens. One of the major peanut allergens, Ara h I, was selected from these clones using Ara h I specific oligonucleotides and polymerase chain reaction technology. The Ara h I clone identified a 2.3-kb mRNA species on a Northern blot containing peanut poly (A)+ RNA. DNA sequence analysis of the cloned inserts revealed that the Ara h I allergen has significant homology with the vicilin seed storage protein family found in most higher plants. The isolation of the Ara h I clones allowed the synthesis of this protein in E. coli cells and subsequent recognition of this recombinant protein in immunoblot analysis using serum IgE from patients with peanut hypersensitivity. With the production of the recombinant peanut protein it will now be possible to address the pathophysiologic and immunologic mechanisms regarding peanut hypersensitivity reactions specifically and food hypersensitivity in general
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Citations
Cites
Fatal and Near-Fatal Anaphylactic Reactions to Food in Children and Adolescents
Sampson, H. A., Mendelson, L., & Rosen, J. P. (1992) · New England Journal of Medicine · doi:10.1056/nejm199208063270603 · Cited by 1,609 · cited 2×
“Multiple cases of fatal anaphylaxis have been reported (4).”
Cited by
Composition, physicochemical properties of pea protein and its application in functional foods
Lu, Z. X., He, J. F., Zhang, Y. C., & Bing, D. J. (2020) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2019.1651248 · Cited by 529
The effects of roasting on the allergenic properties of peanut proteins
Maleki, S. J., Chung, S.-Y., Champagne, E. T., & Raufman, J.-P. (2000) · Journal of Allergy and Clinical Immunology · doi:10.1067/mai.2000.109620 · Cited by 507
Molecular cloning and epitope analysis of the peanut allergen Ara h 3
Rabjohn, P., Helm, E. M., Stanley, J. S., West, C. M., Sampson, H. A., Burks, A. W., & Bannon, G. A. (1999) · Journal of Clinical Investigation · doi:10.1172/jci5349 · Cited by 405
Self-citationShreffler, W. G., Beyer, K., Chu, T.-H. T., Burks, A. W., & Sampson, H. A. (2004) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2003.12.588 · Cited by 349
Self-citationBurks, A. W., Shin, D., Cockrell, G., Stanley, J. S., Helm, R. M., & Bannon, G. A. (1997) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1997.t01-1-00334.x · Cited by 320
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Metadata
- Authors
- Burks, A. W., Cockrell, G., Stanley, J. S., Helm, R. M., & Bannon, G. A.
- Venue
- Journal of Clinical Investigation
- Published
- 1995-10-01
- Volume
- 96
- Issue
- 4
- Pages
- 1715-1721
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2138069377
- Cited by
- 221
- References
- 1
- FWCI
- 2.83
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Burks, A. W., Cockrell, G., Stanley, J. S., Helm, R. M., & Bannon, G. A. (1995). Recombinant peanut allergen Ara h I expression and IgE binding in patients with peanut hypersensitivity. Journal of Clinical Investigation. https://doi.org/10.1172/jci118216
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