Pulsatile insulin release from mouse islets occurs in the absence of stimulated entry of Ca2+.
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Pancreatic islets are known to respond to a raise of the glucose concentration with Ca2+ -induced 2-3-min pulses of insulin release. The reports of cyclic variations of circulating insulin in the fasting state made it important to explore whether insulin release is also pulsatile in the absence of stimulated entry of Ca2+. Individual pancreatic islets were isolated from a local colony of ob/ob mice and perifused under conditions allowing dual wavelength recordings of the cytoplasmic Ca2+ concentration ([Ca2+]i) with fura-2 and measurements of insulin with ELISA technique. At 3 mM of glucose, [Ca2+]i remained at a stable low level, but insulin was released in pulses with a frequency of 0.41+/-0.02 min-1, determined by Fourier transformation of original and autocorrelated data. Pulses of basal insulin release were also seen when glucose was omitted and 1 microM clonidine or 400 microM diazoxide was added to a glucose-free medium. The results indicate that pulsatile insulin release can be generated in the absence of stimulated entry of Ca2+. A tentative explanation for this phenomenon is inherent fluctuations in the ATP production of the beta cells.
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Increased metabolism can stimulate insulin release independent of [Ca2+]i elevation.
“In addition, there is evidence that increased metabolism can stimulate insulin release independent of [Ca 2ϩ ] i elevation (36,37).”
From Paradoxical Stimulation of Glucagon Secretion by High Glucose Concentrations (2006) · cites this paper as [36]
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- Authors
- Westerlund, J., Hellman, B., & Bergsten, P.
- Affiliations
- Uppsala University (SE)
- Corresponding author
- Bergsten, P
- ORCID
- Bergsten, P
- Venue
- Journal of Clinical Investigation
- Published
- 1996-04-15
- Volume
- 97
- Issue
- 8
- Pages
- 1860-1863
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC507254
- OpenAlex
- W2028156250
- Cited by
- 34
- References
- 25
- Citations by year
- 2014: 3 · 2013: 2
- FWCI
- 3.74
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Novo Nordisk
- Stiftelsen Familjen Ernfors Fond
- Novo Nordisk Fonden
- Medical Research Council
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Keywords
- pancreatic islets, intracellular calcium, pancreatic beta cells, ob/ob mice, clonidine, diazoxide, glucose stimulation
- MeSH terms
- Insulin Secretion, Animals, Calcium, Cells, Cultured, Clonidine, Diazoxide, Enzyme-Linked Immunosorbent Assay, Fourier Analysis, Glucose, Insulin, Islets of Langerhans, Kinetics, Mice, Obese, Time Factors, Mice
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