Glucose-Induced Amplitude Regulation of Pulsatile Insulin Secretion From Individual Pancreatic Islets
Abstract
The insulin secretory response to glucose was studied in single pancreatic islets isolated from ob/ob mice and rats. The perfusate from an individual islet was collected during 18-s periods and analyzed for insulin with an ELISA technique. Increase of the glucose concentration from 3 to ≥ 5.5 mM resulted in pulses of insulin release often originating from the basal level and having a frequency of 0.4/min. Glucose regulation of insulin release from the individual islet was manifested by alterations of the amplitudes of the pulses but not of their frequency. It is concluded that the large amplitude oscillations of cytoplasmic Ca2+ known to occur in the pancreatic β-cells have their counterpart in pulses of insulin release and that glucose stimulation of the secretory activity may be the result of recruitment of more β-cells into an oscillatory state.
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Traditional biochemical assays of insulin secretion allow measurements from individual islets with a temporal resolution as high as 20 s.
“Traditional biochemical assays of insulin secretion allow (at best) measurements from individual islets with a temporal resolution as high as 20 s (61).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [61]
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Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men
Rorsman, P., & Ashcroft, F. M. (2018) · Physiological Reviews · doi:10.1152/physrev.00008.2017 · Cited by 793 · see citing works
Pulsatile Insulin Secretion: Detection, Regulation, and Role in Diabetes
Pørksen, N., Hollingdal, M., Juhl, C., Butler, P., Veldhuis, J. D., & Schmitz, O. (2002) · Diabetes · doi:10.2337/diabetes.51.2007.s245 · Cited by 223 · see citing works
Connexins: Key Mediators of Endocrine Function
Bosco, D., Haefliger, J.-A., & Meda, P. (2011) · Physiological Reviews · doi:10.1152/physrev.00027.2010 · Cited by 169 · see citing works
Bertram, R., Satin, L. S., Pedersen, M. G., Luciani, D. S., & Sherman, A. (2007) · Biophysical Journal · doi:10.1529/biophysj.106.097154 · Cited by 105 · see citing works
Pulsatility of insulin release – a clinically important phenomenon
Hellman, B. (2009) · Upsala Journal of Medical Sciences · doi:10.3109/03009730903366075 · Cited by 77 · see citing works
Self-citationPulsatile insulin release from mouse islets occurs in the absence of stimulated entry of Ca2+.
Westerlund, J., Hellman, B., & Bergsten, P. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118616 · Cited by 34 · see citing works
Self-citationSol, E.-R. M., Sargsyan, E., Akusjärvi, G., & Bergsten, P. (2008) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2008.08.013 · Cited by 23 · see citing works
Self-citation
- Authors
- Bergsten, P., & Hellman, B.
- Affiliations
- Uppsala University (SE)
- ORCID
- Bergsten, Peter
- Venue
- Diabetes
- Publisher
- American Diabetes Association
- Published
- 1993-05-01
- Volume
- 42
- Issue
- 5
- Pages
- 670-674
- ISSN
- 0012-1797 (ISSN-L)
- 1939-327X
- OpenAlex
- W2151706465
- Cited by
- 146
- References
- 0
- Citations by year
- 2023: 3 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 3 · 2017: 4 · 2016: 3 · 2015: 3 · 2014: 10 · 2013: 5
- FWCI
- 6.17
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Diabetes (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Vetenskapsrådet
- Association pour la Recherche sur le Diabète
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
- Keywords
- insulin secretion, glucose stimulation, pancreatic islets, pancreatic beta cells, calcium oscillations, ob/ob mice, islet heterogeneity
- MeSH terms
- Insulin Secretion, Animals, Cells, Cultured, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Glucose, Insulin, Islets of Langerhans, Kinetics, Time Factors, Rats, Sprague-Dawley, Mice, Rats
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