The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Delta-aminolevulinic acid transport by intestinal and renal peptide transporters and its physiological and clinical implications.
Open accessAbstract
Delta-aminolevulinic acid (ALA) is the precursor of porphyrin synthesis and has been recently used in vitro and in clinical studies as an endogenous photosensitizer for photodynamic therapy in the treatment of various tumors. For this purpose, ALA is given topically, systemically, or orally. When administered by the oral route, it shows excellent intestinal absorption. ALA is also efficiently reabsorbed in the renal proximal tubule after glomerular filtration. However, the pathways and mechanisms for its transmembrane transport into epithelial cells of intestine and kidney are unknown. Here we demonstrate that ALA uses the intestinal and renal apical peptide transporters for entering into epithelial cells. Kinetics and characteristics of ALA transport were determined in Xenopus laevis ooyctes and Pichia pastoris yeast cells expressing either the cloned intestinal peptide transporter PEPT1 or the renal form PEPT2. By using radiolabeled ALA and electrophysiological techniques in these heterologous expression systems, we established that: (a) PEPT1 and PEPT2 translocate 3H-ALA by saturable and pH-dependent transport mechanisms, (b) that ALA and di-/tripeptides, but not GABA or related amino acids, compete at the same substrate-binding site of the carriers, and (c) that ALA transport is electrogenic in nature as a consequence of H+/ALA cotransport. Reverse transcriptase-PCR analysis performed with specific primers for PEPT1 and PEPT2 in rabbit tissues demonstrates that, in particular, the PEPT2 mRNA is expressed in a variety of other tissues including lung, brain, and mammary gland, which have been shown to accumulate ALA. This suggests that these tissues could take up the porphyrin precusor via expressed peptide transporters, providing the endogenous photosensitizers for efficient photodynamic therapy.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Expression cloning of a mammalian proton-coupled oligopeptide transporter
Fei, Y.-J., Kanai, Y., Nussberger, S., Ganapathy, V., Leibach, F. H., Romero, M. F., Singh, S. K., Boron, W. F., & Hediger, M. A. (1994) · Nature · doi:10.1038/368563a0 · Cited by 891
Human Intestinal H+/Peptide Cotransporter
Liang, R., Fei, Y.-J., Prasad, P. D., Ramamoorthy, S., Han, H., Yang-Feng, T. L., Hediger, M. A., Ganapathy, V., & Leibach, F. H. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.12.6456 · Cited by 502
Oral versus intravenous administration of 5-aminolaevulinic acid for photodynamic therapy
Loh, C., MacRobert, A., Bedwell, J., Regula, J., Krasner, N., & Bown, S. (1993) · British Journal of Cancer · doi:10.1038/bjc.1993.284 · Cited by 263
Molecular cloning of PEPT 2, a new member of the H+/peptide cotransporter family, from human kidney
Liu, W., Liang, R., Ramamoorthy, S., Fei, Y.-J., Ganapathy, M. E., Hediger, M. A., Ganapathy, V., & Leibach, F. H. (1995) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(95)80036-f · Cited by 260
The PTR family: a new group of peptide transporters
Steiner, H., Naider, F., & Becker, J. M. (1995) · Molecular Microbiology · doi:10.1111/j.1365-2958.1995.tb02310.x · Cited by 243
Saito, H., Okuda, M., Terada, T., Sasaki, S., & Inui, K. (1995) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)12227-1 · Cited by 242
MOLECULAR BIOLOGY OF MAMMALIAN AMINO ACID TRANSPORTERS
Malandro, M. S., & Kilberg, M. S. (1996) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.65.070196.001513 · Cited by 210
Boll, M., Herget, M., Wagener, M., Weber, W. M., Markovich, D., Biber, J., Clauss, W., Murer, H., & Daniel, H. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.1.284 · Cited by 192
Self-citationBoll, M., Markovich, D., Weber, W. M., Korte, H., Daniel, H., & Murer, H. (1994) · Pflügers Archiv European Journal of Physiology · doi:10.1007/bf02584043 · Cited by 191
Self-citationMechanisms of the Human Intestinal H+-coupled Oligopeptide Transporter hPEPT1
Mackenzie, B., Loo, D. D. F., Fei, Y.-J., Liu, W., Ganapathy, V., Leibach, F. H., & Wright, E. M. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.10.5430 · Cited by 160
Cited by
Transport of Milk Constituents by the Mammary Gland
Shennan, D. B., & Peaker, M. (2000) · Physiological Reviews · doi:10.1152/physrev.2000.80.3.925 · Cited by 411
Contribution of microbial amino acids to amino acid homeostasis of the host
Metges, C. C. (2000) · The Journal of Nutrition · doi:10.1093/jn/130.7.1857s · Cited by 391
5‐Aminolevulinic Acid Derivatives in Photomedicine: Characteristics, Application and Perspectives
Fotinos, N., Campo, M. A., Popowycz, F., Gurny, R., & Lange, N. (2006) · Photochemistry and Photobiology · doi:10.1562/2006-02-03-ir-794 · Cited by 238
Transport of 5-aminolevulinic acid between blood and brain
Ennis, S. R., Novotny, A., Xiang, J., Shakui, P., Masada, T., Stummer, W., Smith, D. E., & Keep, R. F. (2003) · Brain Research · doi:10.1016/s0006-8993(02)03749-6 · Cited by 146
Kitajima, Y., Ishii, T., Kohda, T., Ishizuka, M., Yamazaki, K., Nishimura, Y., Tanaka, T., Dan, S., & Nakajima, M. (2019) · Scientific Reports · doi:10.1038/s41598-019-44981-y · Cited by 56
Metadata
- Authors
- Döring, F., Walter, J., Will, J., Föcking, M., Boll, M., Amasheh, S., Clauss, W., & Daniel, H.
- Venue
- Journal of Clinical Investigation
- Published
- 1998-06-15
- Volume
- 101
- Issue
- 12
- Pages
- 2761-2767
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W1992404214
- Cited by
- 269
- References
- 29
- FWCI
- 5.34
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Delta-aminolevulinic acid transport by intestinal and renal peptide transporters and its physiological and clinical implications. https://app.cochat.ai/papers/10.1172/jci1909
- The paper
- Döring, F., Walter, J., Will, J., Föcking, M., Boll, M., Amasheh, S., Clauss, W., & Daniel, H. (1998). Delta-aminolevulinic acid transport by intestinal and renal peptide transporters and its physiological and clinical implications. Journal of Clinical Investigation. https://doi.org/10.1172/jci1909
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
