Progress toward the clinical application of patient-specific pluripotent stem cells
Open accessAbstract
Induced pluripotent stem (iPS) cells are generated by epigenetic reprogramming of somatic cells through the exogenous expression of transcription factors. These cells, just like embryonic stem cells, are likely to have a major impact on regenerative medicine, because they self-renew and retain the potential to be differentiated into all cell types of the human body. In this Review, we describe the current state of iPS cell technology, including approaches by which they are generated and what is known about their biology, and discuss the potential applications of these cells for disease modeling, drug discovery, and, eventually, cell replacement therapy.
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Reprogramming without viral integration using plasmids or direct reprogramming protein delivery assays can solve the oncogene issue.
“Nonetheless, reprogramming without viral integration with plasmids or direct reprogramming protein delivery assays can solve this issue [98,99].”
From Non-Integrating Lentiviral Vectors in Clinical Applications: A Glance Through (2022) · cites this paper as [98]
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“…development, have undergone directed differentiation into astrocytes and neuronal subtypes of the CNS, and even been used for cellular transplantation therapies for neurodegenerative diseases (Park et al., 2008a; Kiskinis and Eggan, 2010; Wu and Hochedlinger, 2011; Ben-David et al., 2012).”
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- Authors
- Kiskinis, E., & Eggan, K.
- Affiliations
- Harvard University (US)
- Venue
- Journal of Clinical Investigation
- Published
- 2010-01-04
- Volume
- 120
- Issue
- 1
- Pages
- 51-59
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC2798698
- OpenAlex
- W1986371962
- Cited by
- 339
- References
- 7
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 5 · 2023: 4 · 2022: 9 · 2021: 7 · 2020: 13 · 2019: 8 · 2018: 13 · 2017: 12 · 2016: 20 · 2015: 29 · 2014: 30 · 2013: 39 · 2012: 42
- FWCI
- 5.14
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
- Funders
- Howard Hughes Medical Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- induced pluripotent stem cells, epigenetic reprogramming, transcription factors, pluripotency, regenerative medicine, disease modeling, drug discovery, cell replacement therapy, clinical application
- MeSH terms
- Amyotrophic Lateral Sclerosis, Animals, Cell Differentiation, Humans, Parkinson Disease, Pluripotent Stem Cells, Mice, Embryonic Stem Cells, Drug Discovery
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