The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Vasopressin-induced von Willebrand factor secretion from endothelial cells involves V2 receptors and cAMP
Open accessAbstract
Vasopressin and its analogue 1-deamino-8-D-arginine vasopressin (DDAVP) are known to raise plasma von Willebrand factor (vWF) levels. DDAVP is used as a hemostatic agent for the treatment of von Willebrand's disease. However, its cellular mechanisms of action have not been elucidated. DDAVP, a specific agonist for the vasopressin V2 receptor (V2R), exerts its antidiuretic effect via a rise in cAMP in kidney collecting ducts. We tested the hypothesis that DDAVP induces vWF secretion by binding to V2R and activating cAMP-mediated signaling in endothelial cells. vWF secretion from human umbilical vein endothelial cells (HUVECs) can be mediated by cAMP, but DDAVP is ineffective, presumably due to the absence of V2R. We report that DDAVP stimulates vWF secretion in a cAMP-dependent manner in HUVECs after transfection of the V2R. In addition, vasopressin and DDAVP induce vWF secretion in human lung microvascular endothelial cells (HMVEC-L). These cells (but not HUVECs) express endogenous V2R, as shown by RT-PCR. Vasopressin-induced vWF secretion is mimicked by DDAVP and inhibited by the selective V2R antagonist SR121463B. It is mediated by cAMP, since it is inhibited by the protein kinase A inhibitor Rp-8CPT-cAMPS. These results indicate that vasopressin induces cAMP-mediated vWF secretion by a direct effect on endothelial cells. They also demonstrate functional expression of V2R in endothelial cells, and provide a cellular mechanism for the hemostatic effects of DDAVP.
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“Vasopressin is a neurohormone that is synthesized in the hypothalamus and transported to the posterior pituitary (Brownstein et al., 1980), where it is released into the bloodstream during dehydration to act via vasopressin V 2 receptors on the kidneys to produce water retention (Kaufmann et al., 2000).”
From Drug Addiction: Hyperkatifeia/Negative Reinforcement as a Framework for Medications Development (2021) · cites this paper as Kaufmann et al., 2000
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Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
BIOCHEMISTRY AND GENETICS OF VON WILLEBRAND FACTOR
Sadler, J. E. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.395 · Cited by 1,369
IRON AND THE SEX DIFFERENCE IN HEART DISEASE RISK
Sullivan, J. (1981) · The Lancet · doi:10.1016/s0140-6736(81)92463-6 · Cited by 969
Immunolocalization of von Willebrand protein in Weibel-Palade bodies of human endothelial cells
Wagner, D. D. (1982) · The Journal of Cell Biology · doi:10.1083/jcb.95.1.355 · Cited by 703
Mannucci, P. M., Pareti, F. I., Ruggeri, Z. M., & Capitanio, A. (1977) · The Lancet · doi:10.1016/s0140-6736(77)91197-7 · Cited by 604
Molecular cloning of the receptor for human antidiuretic hormone
Birnbaumer, M., Seibold, A. M., Gilbert, S., Ishido, M., Barberis, C., Antaramián, A., Brabet, P., & Rosenthal, W. (1992) · Nature · doi:10.1038/357333a0 · Cited by 539
Lolait, S. J., O'Carroll, A.-M., McBride, O. W., Konig, M., Morel, A., & Brownstein, M. J. (1992) · Nature · doi:10.1038/357336a0 · Cited by 524 · cited 1×
“This vasopressin receptor subtype is expressed in the principal cells of renal collecting ducts and mediates the antidiuretic effect of the hormone (7, 8).”
BackgroundDesmopressin (DDAVP) in the Treatment of Bleeding Disorders: The First 20 Years
Mannucci, P. M. (1997) · Blood · doi:10.1182/blood.v90.7.2515 · Cited by 436
Desmopressin (DDAVP) in the Treatment of Bleeding Disorders: The First 20 Years
Mannucci, P. M. (1997) · Blood · doi:10.1182/blood.v90.7.2515.2515_2515_2521 · Cited by 398
Mechanism of Plasminogen Activator and Factor VIII Increase after Vasoactive Drugs
Mannucci, P. M., Åberg, M., Nilsson, I. M., & Robertson, B. (1975) · British Journal of Haematology · doi:10.1111/j.1365-2141.1975.tb00521.x · Cited by 391
Cited by
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Nichols, W. L., Hultin, M., James, A., Manco‐Johnson, M. J., Montgomery, R. R., Ortel, T. L., Rick, M. E., Sadler, J. E., Weinstein, M., & Yawn, B. P. (2008) · Haemophilia · doi:10.1111/j.1365-2516.2007.01643.x · Cited by 967
Treatment of von Willebrand's Disease
Mannucci, P. M. (2004) · New England Journal of Medicine · doi:10.1056/nejmra040403 · Cited by 483
The vessel wall and its interactions
Wagner, D. D., & Frenette, P. S. (2008) · Blood · doi:10.1182/blood-2008-01-078204 · Cited by 357
Dynamics and Plasticity of Weibel-Palade Bodies in Endothelial Cells
Rondaij, M. G., Bierings, R., Kragt, A., van MOURIK, J. A., & Voorberg, J. (2006) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000209501.56852.6c · Cited by 341
von Willebrand factor, endothelial dysfunction, and cardiovascular disease
Vischer, U. M. (2006) · Journal of Thrombosis and Haemostasis · doi:10.1111/j.1538-7836.2006.01949.x · Cited by 351
Metadata
- Authors
- Kaufmann, J. E., Oksche, A., Wollheim, C. B., Günther, G., Rosenthal, W., & Vischer, U. M.
- Affiliations
- University of Geneva (CH)
- Leibniz-Forschungsinstitut für Molekulare Pharmakologie (DE)
- Corresponding author
- Kaufmann, Jocelyne E.
- Venue
- Journal of Clinical Investigation
- Published
- 2000-07-01
- Volume
- 106
- Issue
- 1
- Pages
- 107-116
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC314363
- OpenAlex
- W2052753575
- Cited by
- 275
- References
- 47
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 6 · 2023: 14 · 2022: 11 · 2021: 12 · 2020: 12 · 2019: 9 · 2018: 15 · 2017: 7 · 2016: 13 · 2015: 12 · 2014: 13 · 2013: 15 · 2012: 8
- FWCI
- 4.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Archive ouverte UNIGE (University of Geneva) (publishedVersion, other-oa)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
- Funders
- Fonds National de la Recherche Luxembourg
- Helmut Horten Stiftung
- Topics
- Health Sciences › Medicine › Hematology › Platelet Disorders and Treatments
- Health Sciences › Medicine › Surgery › Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Health Sciences › Medicine › Hematology › Blood Coagulation and Thrombosis Mechanisms
- Keywords
- vasopressin, von Willebrand factor secretion, V2 receptor, cAMP signaling, protein kinase A, endothelial cells, desmopressin, von Willebrand disease, hemostasis, lung microvascular endothelial cells
- MeSH terms
- Cyclic AMP, Arginine Vasopressin, Cells, Cultured, Deamino Arginine Vasopressin, Endothelium, Vascular, Humans, Lung, RNA, Messenger, von Willebrand Factor, Receptors, Vasopressin, Reverse Transcriptase Polymerase Chain Reaction
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