The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Validation of an in vitro Biofilm Model of Supragingival Plaque
Abstract
The study of biofilm structure and function mandates the use of model systems for which a host of environmental variables can be rigorously controlled. We describe a model of supragingival plaque containing Actinomyces naeslundii, Veillonella dispar, Fusobacterium nucleatum, Streptococcus sobrinus, and Streptococcus oralis wherein cells are cultivated anaerobically in a saliva-based medium on hydroxyapatite discs coated with a salivary pellicle, with material and pieces of apparatus common to all microbiology laboratories. After 0.5 hr, 16.5 hrs, 40.5 hrs, and 64.5 hrs, the composition of adherent biofilms was analyzed by culture techniques, live/dead fluorescence staining, and confocal laser scanning microscopy. Repeated independent trials demonstrated the repeatability of biofilm formation after 40.5 hrs and 64.5 hrs. Brief exposures of biofilms to chlorhexidine or Triclosan produced losses in viability similar to those observed in vivo. This biofilm model should prove very useful for pre-clinical testing of prospective anti-plaque agents at clinically relevant concentrations.
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Cites
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Self-citationThe effect of water-soluble polymers on drug-cyclodextrin complexation
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Self-citationEvolving perspectives of biofilm structure
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Cited by
Antimicrobial photodynamic therapy – what we know and what we don’t
Cieplik, F., Deng, D., Crielaard, W., Buchalla, W., Hellwig, E., Al-Ahmad, A., & Maisch, T. (2018) · Critical Reviews in Microbiology · doi:10.1080/1040841x.2018.1467876 · Cited by 902
Koo, H., Xiao, J., Klein, M. I., & Jeon, J. G. (2010) · Journal of Bacteriology · doi:10.1128/jb.01649-09 · Cited by 508
Propidium iodide staining underestimates viability of adherent bacterial cells
Rosenberg, M., Azevedo, N. F., & Ivask, A. (2019) · Scientific Reports · doi:10.1038/s41598-019-42906-3 · Cited by 447
Tavassoli Hojati, S., Alaghemand, H., Hamze, F., Ahmadian Babaki, F., Rajab-Nia, R., Rezvani, M. B., Kaviani, M., & Atai, M. (2013) · Dental Materials · doi:10.1016/j.dental.2013.03.011 · Cited by 305
Mass Transport of Macromolecules within an In Vitro Model of Supragingival Plaque
Thurnheer, T., Gmür, R., Shapiro, S., & Guggenheim, B. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.3.1702-1709.2003 · Cited by 145
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Metadata
- Authors
- Guggenheim, B., Giertsen, E., Schüpbach, P., & Shapiro, S.
- Venue
- Journal of Dental Research
- Publisher
- SAGE Publications
- Published
- 2001-01
- Volume
- 80
- Issue
- 1
- Pages
- 363-370
- ISSN
- 0022-0345 (ISSN-L)
- 1544-0591
- OpenAlex
- W2139318392
- Cited by
- 357
- References
- 23
- FWCI
- 12.71
- Type
- journal article
- Language
- en
- Open access
- closed
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- Guggenheim, B., Giertsen, E., Schüpbach, P., & Shapiro, S. (2001). Validation of an in vitro Biofilm Model of Supragingival Plaque. Journal of Dental Research. https://doi.org/10.1177/00220345010800011201
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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