Mass Transport of Macromolecules within an In Vitro Model of Supragingival Plaque

Thurnheer, Thomas; Gmür, Rudolf; Shapiro, Stuart; Guggenheim, Bernhard

doi:10.1128/aem.69.3.1702-1709.2003

Cited by 145Oral microbiology and periodontitis researchBacterial biofilms and quorum sensingDental Health and Care Utilization

Mass Transport of Macromolecules within an In Vitro Model of Supragingival Plaque

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Thurnheer, Thomas, Gmür, Rudolf, Shapiro, Stuart, Guggenheim, Bernhard·2003·Applied and Environmental Microbiology

DOI 10.1128/aem.69.3.1702-1709.2003PMC PMC150054

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Abstract

The aim of this study was to examine the diffusion of macromolecules through an in vitro biofilm model of supragingival plaque. Polyspecies biofilms containing Actinomyces naeslundii , Fusobacterium nucleatum , Streptococcus oralis , Streptococcus sobrinus , Veillonella dispar , and Candida albicans were formed on sintered hydroxyapatite disks and then incubated at room temperature for defined periods with fluorescent markers with molecular weights ranging from 3,000 to 900,000. Subsequent examination by confocal laser scanning microscopy revealed that the mean square penetration depths for all tested macromolecules except immunoglobulin M increased linearly with time, diffusion coefficients being linearly proportional to the cube roots of the molecular weights of the probes (range, 10,000 to 240,000). Compared to diffusion in bulk water, diffusion in the biofilms was markedly slower. The rate of diffusion for each probe appeared to be constant and not a function of biofilm depth. Analysis of diffusion phenomena through the biofilms suggested tortuosity as the most probable explanation for retarded diffusion. Selective binding of probes to receptors present in the biofilms could not explain the observed extent of retardation of diffusion. These results are relevant to oral health, as selective attenuated diffusion of fermentable carbohydrates and acids produced within dental plaque is thought to be essential for the development of carious lesions.

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Thurnheer et al found that penetration depths of macromolecules decreased linearly for molecular weights up to 240,000 Da in Zurich biofilm model of supragingival plaque.
“Thurnheer et al242 found that penetration depths of macromolecules decreased linearly for molecular weights up to 240,000 Da in their Zurich biofilm model of supragingival plaque.”
From The dental plaque biofilm matrix (2021) · cites this paper as [242]
Mixed-species biofilms cultured in vitro retard the penetration of immunoglobulin G (IgG) and immunoglobulin M antibodies, to the point where IgG does not reach the center of clusters a few hundred micrometers in diameter.
“Mixed-species biofilms cultured in vitro retard the penetration of immunoglobulin G (IgG) and immunoglobulin M antibodies, to the point where IgG does not reach the center of clusters a few hundred micrometers in diameter.242,250”
From The dental plaque biofilm matrix (2021) · cites this paper as [242]
Exopolysaccharides create chemical and metabolic gradients within biofilms that affect chemical and nutrient cycling as well as diffusion, thus altering the microenvironment and pH within the biofilm consortium.
“Furthermore, exopolysaccharides create chemical and metabolic gradients within biofilms that affect chemical and nutrient cycling as well as diffusion, thus altering the microenvironment and pH within biofilms consortium.”
From The oral microbiome: Role of key organisms and complex networks in oral health and disease (2021) · cites this paper as [287]

Über die von der molekularkinetischen Theorie der Wärme geforderte Bewegung von in ruhenden Flüssigkeiten suspendierten Teilchen
Einstein, A. (1905) · Annalen der Physik · doi:10.1002/andp.19053220806 · Cited by 8,422 · see citing works
MICROBIAL BIOFILMS
Costerton, J. W., Lewandowski, Z., Caldwell, D. E., Korber, D. R., & Lappin-Scott, H. M. (1995) · Annual Review of Microbiology · doi:10.1146/annurev.mi.49.100195.003431 · Cited by 4,718 · see citing works
An Introduction to Error Analysis
Taylor, J. R. (1982) · Medical Entomology and Zoology · Cited by 2,807 · see citing works
An Introduction to Error Analysis: The Study of Uncertainties in Physical Measurements
Taylor, J. R., & Thompson, W. (1998) · Physics Today · doi:10.1063/1.882103 · Cited by 2,734 · see citing works
Zur kinetischen Theorie der Brownschen Molekularbewegung und der Suspensionen
Smoluchowski, M. (1906) · Annalen der Physik · doi:10.1002/andp.19063261405 · Cited by 1,515 · see citing works
Ion diffusion modified by tortuosity and volume fraction in the extracellular microenvironment of the rat cerebellum.
Nicholson, C., & Phillips, J. M. (1981) · The Journal of Physiology · doi:10.1113/jphysiol.1981.sp013981 · Cited by 785 · see citing works
Diffusion and related transport mechanisms in brain tissue
Nicholson, C. (2001) · Reports on Progress in Physics · doi:10.1088/0034-4885/64/7/202 · Cited by 491 · see citing works
Validation of an in vitro Biofilm Model of Supragingival Plaque
Guggenheim, B., Giertsen, E., Schüpbach, P., & Shapiro, S. (2001) · Journal of Dental Research · doi:10.1177/00220345010800011201 · Cited by 358 · see citing works
Self-citation
Distribution of sulfate-reducing bacteria, O2, and H2S in photosynthetic biofilms determined by oligonucleotide probes and microelectrodes
Ramsing, N. B., Kuhl, M. E., & Jørgensen, B. B. (1993) · Applied and Environmental Microbiology · doi:10.1128/aem.59.11.3840-3849.1993 · Cited by 275 · see citing works
Salivary α-Amylase: Role in Dental Plaque and Caries Formation
Scannapieco, F. A., Torres, G., & Levine, M. J. (1993) · Critical Reviews in Oral Biology & Medicine · doi:10.1177/10454411930040030701 · Cited by 272 · see citing works
Hindered diffusion of high molecular weight compounds in brain extracellular microenvironment measured with integrative optical imaging
Nicholson, C., & Tao, L. (1993) · Biophysical Journal · doi:10.1016/s0006-3495(93)81324-9 · Cited by 266 · see citing works
Hereditary fructose intolerance
Froesch, E. R., Wolf, H. P., Baitsch, H., Prader, A., & Labhart, A. (1963) · The American Journal of Medicine · doi:10.1016/0002-9343(63)90050-0 · Cited by 254 · see citing works
Food Starches and Dental Caries
Lingström, P., van Houte, J., & Kashket, S. (2000) · Critical Reviews in Oral Biology & Medicine · doi:10.1177/10454411000110030601 · Cited by 238 · see citing works
Determination of Diffusion Coefficients in Biofilms by Confocal Laser Microscopy
Lawrence, J. R., Wolfaardt, G. M., & Korber, D. R. (1994) · Applied and Environmental Microbiology · doi:10.1128/aem.60.4.1166-1173.1994 · Cited by 209 · see citing works
An in vitro Oral Biofilm Model for Comparing the Efficacy of Antimicrobial Mouthrinses
Shapiro, S., Giertsen, E., & Guggenheim, B. (2002) · Caries Research · doi:10.1159/000057866 · Cited by 190 · see citing works
Self-citation
Immobilized cells: basics and applications.
Wijffels, R. H., Buitelaar, R. M., Bucke, C., & TRAMPER, J. (1996) · Socio-Environmental Systems Modeling · Cited by 188 · see citing works
Entropic trapping of macromolecules by mesoscopic periodic voids in a polymer hydrogel
Liu, L., Li, P., & Asher, S. A. (1999) · Nature · doi:10.1038/16426 · Cited by 186 · see citing works
Antigenic heterogeneity of Bacteroides intermedius as recognized by monoclonal antibodies
Gmür, R., & Guggenheim, B. (1983) · Infection and Immunity · doi:10.1128/iai.42.2.459-470.1983 · Cited by 183 · see citing works
Self-citation
Distribution of Sulfate-Reducing and Methanogenic Bacteria in Anaerobic Aggregates Determined by Microsensor and Molecular Analyses
Santegoeds, C. M., Damgaard, L. R., Hesselink, G., Zopfi, J., Lens, P. N. L., Gerard Muyzer, C., & de Beer, D. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.10.4618-4629.1999 · Cited by 153 · see citing works
Sugar and Dental Caries: A Review of Human Studies
Newbrun, E. (1982) · Science · doi:10.1126/science.7046052 · Cited by 137 · see citing works
Physiological Approaches to the Control of Oral Biofilms
Marsh, P. D., & Bradshaw, D. J. (1997) · Advances in Dental Research · doi:10.1177/08959374970110010901 · Cited by 132 · see citing works
Microelectrode measurements of local mass transport rates in heterogeneous biofilms
Rasmussen, K., & Lewandowski, Z. (1998) · Biotechnology and Bioengineering · doi:10.1002/(sici)1097-0290(19980805)59:3 · Cited by 130 · see citing works
Diffusional transport of macromolecules in developing nerve processes
Popov, S., & Poo, M. (1992) · Journal of Neuroscience · doi:10.1523/jneurosci.12-01-00077.1992 · Cited by 120 · see citing works
Isolation of a glucan-binding domain of glucosyltransferase (1,6-alpha-glucan synthase) from Streptococcus sobrinus
Mooser, G., & Wong, C. (1988) · Infection and Immunity · doi:10.1128/iai.56.4.880-884.1988 · Cited by 114 · see citing works
Dental plaque revisited: oral biofilms in health and disease
Wilson, M., & Wilson, M. (1998) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.1998.tb02341.x · Cited by 114 · see citing works
Carbohydrates in Pooled Dental Plaque
Hotz, P., Guggenheim, B., & Schmid, R. (2009) · Caries Research · doi:10.1159/000259783 · Cited by 110 · see citing works
Self-citation
Local macromolecule diffusion coefficients in structurally non-uniform bacterial biofilms using fluorescence recovery after photobleaching (FRAP)
Bryers, J. D., & Drummond, F. (1998) · Biotechnology and Bioengineering · doi:10.1002/(sici)1097-0290(19981120)60:4 · Cited by 110 · see citing works
Heterogeneity of Diffusion Inside Microbial Biofilms Determined by Fluorescence Correlation Spectroscopy Under Two-photon Excitation¶
Guiot, E., Georges, P., Brun, A., Fontaine‐Aupart, M., Bellon‐Fontaine, M., & Briandet, R. (2002) · Photochemistry and Photobiology · doi:10.1562/0031-8655(2002)0750570hodimb2.0.co2 · Cited by 99 · see citing works
Evidence that tropical soil environment can support the growth of Escherichia coli
Byappanahalli, M. N., & Fujioka, R. S. (1998) · Water Science & Technology · doi:10.2166/wst.1998.0533 · Cited by 91 · see citing works
Morphological characterization of filamentous microorganisms in submerged cultures by on-line digital image analysis and pattern recognition
Treskatis, S. ‐., Orgeldinger, V., Wolf, H., & Gilles, E. D. (1997) · Biotechnology and Bioengineering · doi:10.1002/(sici)1097-0290(19970120)53:2 · Cited by 85 · see citing works
Spatial Arrangements and Associative Behavior of Species in an In Vitro Oral Biofilm Model
Guggenheim, M., Shapiro, S., Gmür, R., & Guggenheim, B. (2001) · Applied and Environmental Microbiology · doi:10.1128/aem.67.3.1343-1350.2001 · Cited by 84 · see citing works
Self-citation
Diffusion in Pseudomonas aeruginosa biofilms: a pulsed field gradient NMR study
Vogt, M., Flemming, H., & Veeman, W. S. (2000) · Journal of Biotechnology · doi:10.1016/s0168-1656(99)00213-8 · Cited by 82 · see citing works
Dental plaque revisited--oral biofilms in health and disease.
Newman, H., & Wilson, M. M. (2000) · PubMed · Cited by 80 · see citing works
Diffusion of Dextran in Aqueous (Hydroxypropyl)cellulose
Bu, Z., & Russo, P. S. (1994) · Macromolecules · doi:10.1021/ma00083a017 · Cited by 75 · see citing works
Measurement of local effective diffusivity in heterogeneous biofilms
Beyenal, H., Tanyolaç, A., & Lewandowski, Z. (1998) · Water Science & Technology · doi:10.2166/wst.1998.0804 · Cited by 74 · see citing works
Macromolecules released from polymers: diffusion into unstirred fluids
Radomsky, M. L., Whaley, K. J., Ra, C., & Saltzman, W. M. (1990) · Biomaterials · doi:10.1016/0142-9612(90)90018-l · Cited by 73 · see citing works
Characterization of the diffusive properties of biofilms using pulsed field gradient-nuclear magnetic resonance
Beuling, E. E., van Dusschoten, D., Lens, P. N. L., van den Heuvel, J. C., As, H. V., & Ottengraf, S. P. P. (1998) · Biotechnology and Bioengineering · doi:10.1002/(sici)1097-0290(19981105)60:3 · Cited by 72 · see citing works
Bacterial Metabolism in Dental Biofilms
Carlsson, J. (1997) · Advances in Dental Research · doi:10.1177/08959374970110012001 · Cited by 67 · see citing works
Integration of Raman microscopy, differential interference contrast microscopy, and attenuated total reflection Fourier transform infrared spectroscopy to investigate chlorhexidine spatial and temporal distribution in Candida albicans biofilms
Suci, P. A., Geesey, G. G., & Tyler, B. J. (2001) · Journal of Microbiological Methods · doi:10.1016/s0167-7012(01)00268-8 · Cited by 65 · see citing works
A Chemical and Histological Study of Artificial Caries in Human Dental Enamel in vitro
Ingram, G. S., & Silverstone, L. M. (2009) · Caries Research · doi:10.1159/000260543 · Cited by 62 · see citing works
Crystallohydrodynamics for solving the hydration problem for multi-domain proteins: open physiological conformations for human IgG
Carrasco, B., de la Torre, J. G., Davis, K. G., Jones, S., Athwal, D., Walters, C., Burton, D. R., & Harding, S. E. (2001) · Biophysical Chemistry · doi:10.1016/s0301-4622(01)00220-4 · Cited by 62 · see citing works
Diffusion of sugars and carboxylic acids through human dental plaque in vitro
Dibdin, G. H. (1981) · Archives of Oral Biology · doi:10.1016/0003-9969(81)90010-8 · Cited by 59 · see citing works
The Discovery of a Novel R-phycoerythrin from an Antarctic Red Alga
MacColl, R., Eisele, L. E., Williams, E. C., & Bowser, S. S. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.29.17157 · Cited by 52 · see citing works
Fractal structure of biofilms: new tools for investigation of morphology
(1995) · Water Science & Technology · doi:10.1016/0273-1223(96)00013-3 · Cited by 50 · see citing works
Diffusion of Radio tracers in Human Dental Plaque
Tatevossian, A. (2009) · Caries Research · doi:10.1159/000260396 · Cited by 39 · see citing works
Removal and recovery of lead fixed-bed biosorption with immobilized bacterial biomass
(1998) · Water Science & Technology · doi:10.1016/s0273-1223(98)00526-5 · Cited by 35 · see citing works
Glucan Inhibition of Diffusion in Plaque
Hojo, S., Higuchi, M., & Araya, S. (1976) · Journal of Dental Research · doi:10.1177/00220345760550011501 · Cited by 35 · see citing works
Hereditary fructose intolerance. Dental status of eight patients.
Tm, M., & Froesch, E. R. (1967) · PubMed · Cited by 33 · see citing works
Multidimensional Modeling of Biofilm Structure
Picioreanu, C. (1999) · Research Repository (Delft University of Technology) · Cited by 31 · see citing works
Diffusion and binding measurements within oral biofilms using fluorescence photobleaching recovery methods
Birmingham, J. J., Hughes, N. P., & Treloar, R. (1995) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.1995.0168 · Cited by 27 · see citing works
Effect of extracellular polysaccharides on diffusion of NaF and [14C]-sucrose in human dental plaque and in sediments of the bacterium Streptococcus sanguis 804 (NCTC 10904)
McNee, S. G., Geddes, D. A. M., Weetman, D. A., Sweeney, D., & BEELEY, J. A. (1982) · Archives of Oral Biology · doi:10.1016/0003-9969(82)90107-8 · Cited by 27 · see citing works
Time-resolved study of biofilm architecture and transport processes using experimental and simulation techniques: the role of EPS
Kuehn, M., Mehl, M., Hausner, M., Bungartz, H., & Wuertz, S. (2001) · Water Science & Technology · doi:10.2166/wst.2001.0361 · Cited by 27 · see citing works
Fractal structure of biofilms: new tools for investigation of morphology
Hermanowicz, S. W., Schindler, U., & Wilderer, P. A. (1995) · Water Science & Technology · doi:10.2166/wst.1995.0273 · Cited by 27 · see citing works
A Brief Survey of Recent in vitro Work on Diffusion of Small Ions and Molecules in Dental Plaque
Dibdin, G. H. (2015) · KARGER eBooks · doi:10.1159/000408738 · Cited by 23 · see citing works
Effect of SDS Micelles on Rhodamine-B Diffusion in Hydrogels
Häglund, B. O., Sundelöf, L., Upadrashta, S. M., & Wurster, D. E. (1996) · Journal of Chemical Education · doi:10.1021/ed073p889 · Cited by 19 · see citing works
Characterization of monoclonal antibodies for rapid identification of in clinical samples
Thurnheer, T., Guggenheim, B., & Gmür, R. (1997) · FEMS Microbiology Letters · doi:10.1016/s0378-1097(97)00125-0 · Cited by 15 · see citing works
Self-citation
Characterization of monoclonal antibodies for rapid identification of Actinomyces naeslundii in clinical samples
Thurnheer, T., Guggenheim, B., & Gmür, R. (2006) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1997.tb10378.x · Cited by 15 · see citing works
Self-citation
Fiber-optic microsensors to measure backscattered light intensity in biofilms
Beyenal, H., Lewandowski, Z., Yakymyshyn, C. P., Lemley, B., & Wehri, J. (2000) · Applied Optics · doi:10.1364/ao.39.003408 · Cited by 14 · see citing works
Competition between nitrogen-fixing cyanobacteria during phosphorus-limited growth
Nobel, W. T. D., Staats, N., & Mur, L. R. (1995) · Water Science & Technology · doi:10.1016/0273-1223(95)00685-0 · Cited by 10 · see citing works
Local Mass Transfer Coefficients in Bacterial Biofilms Using Fluorescence Recovery After Photobleaching (FRAP)
Bryers, J. D., & Drummond, F. (1996) · Progress in biotechnology · doi:10.1016/s0921-0423(96)80028-1 · Cited by 6 · see citing works
Water law and human rights - roles and responsibilities
de Lange, M. (2001) · Water Science & Technology · doi:10.2166/wst.2001.0204 · Cited by 6 · see citing works
Biofilm structure and behaviour: influence of hydrodynamics and nutrients
Stoodley, P., Boyle, J. D., & Lappin-Scott, H. M. (1999) · ePrints Soton (University of Southampton) · Cited by 5 · see citing works

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Neu, T. R., & Lawrence, J. R. (2014) · Advances in biochemical engineering, biotechnology · doi:10.1007/10_2014_272 · Cited by 103 · see citing works
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Self-citation
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Jeon, J., Klein, M. I., Xiao, J., Gregoire, S., Rosalen, P. L., & Koo, H. (2009) · BMC Microbiology · doi:10.1186/1471-2180-9-228 · Cited by 73 · see citing works
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Reese, S., & Guggenheim, B. (2007) · Microscopy Research and Technique · doi:10.1002/jemt.20471 · Cited by 71 · see citing works
Self-citation
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Self-citation
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Ma, R., Liu, J., Jiang, Y., Liu, Z., Tang, Z., Ye, D., Zeng, J., & Huang, Z. (2010) · International Journal of Oral Science · doi:10.4248/ijos10075 · Cited by 13 · see citing works
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doi: 10.4248/IJOS10075 Modeling of Diffusion Transport through Oral Biofilms with the Inverse Problem Method
(2010)
Quantifying diffusion in a biofilm of Streptococcus mutans. Antimicrob Agents Chemother
Zhang, Z., Nadezhina, E., & Wilkinson, K. J. (2010)
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Robinson, C. (2011) · Archives of Oral Biology · doi:10.1016/j.archoralbio.2011.02.001 · Cited by 18 · see citing works

Authors: Thurnheer, T., Gmür, R., Shapiro, S., & Guggenheim, B.
Affiliations: University of Zurich (CH)
Institute for Oral Microbiology and General Immunology, University of Zürich, CH-8028 Zürich, Switzerland
ORCID: Thurnheer, Thomas
Shapiro, Stuart
Venue: Applied and Environmental Microbiology
Publisher: American Society for Microbiology
Published: 2003-03
Volume: 69
Issue: 3
Pages: 1702-1709
ISSN: 0099-2240 (ISSN-L)
1098-5336
DOI: 10.1128/aem.69.3.1702-1709.2003
PMC: PMC150054
OpenAlex: W2166083475
Cited by: 145
References: 62
Citations by year: 2026: 1 · 2025: 2 · 2024: 3 · 2023: 7 · 2022: 5 · 2021: 10 · 2020: 5 · 2019: 4 · 2018: 8 · 2017: 3 · 2016: 6 · 2015: 11 · 2014: 6 · 2013: 11 · 2012: 6
FWCI: 9.97
Type: journal article
Language: en
Open access: green
europepmc.org
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Applied and Environmental Microbiology (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Zurich Open Repository and Archive (University of Zurich) (acceptedVersion, other-oa)
Zurich Open Repository and Archive (University of Zurich) (submittedVersion, other-oa)
Zurich Open Repository and Archive (University of Zurich)
Funders: Universität Konstanz
Topics: Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Health Sciences › Dentistry › Periodontics › Dental Health and Care Utilization
Keywords: supragingival plaque, dental plaque, macromolecular diffusion, diffusion coefficient, molecular weight dependence, tortuosity, fluorescent probes, immunoglobulin M, Actinomyces naeslundii, Fusobacterium nucleatum, Streptococcus sobrinus, Streptococcus oralis, Veillonella dispar, Candida albicans, hydroxyapatite, caries development, oral health
MeSH terms: Actinomyces, Albumins, Candida albicans, Dental Plaque, Dextrans, Diffusion, Fluorescent Dyes, Gingiva, Models, Biological, Veillonella, Fusobacterium nucleatum, Streptococcus sobrinus, Biofilms, Streptococcus oralis, Macromolecular Substances

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Mass Transport of Macromolecules within an In Vitro Model of Supragingival Plaque

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