Calibrated histochemistry of myoglobin concentration in cardiomyocytes
Abstract
This article describes the calibration of a histochemical method to determine the myoglobin concentration in individual cardiomyocytes. Calibration is based on paired microdensitometric determinations in sections stained for myoglobin and on biochemical myoglobin determinations in tissue samples from different hearts. In addition, the staining intensity of sections from gelatin blocks containing known amounts of myoglobin is determined. To construct a calibration line, sections stained for myoglobin must be corrected for the degree of shrinkage caused by glutaraldehyde fixation and biochemical myoglobin determinations must be corrected for interstitial space. As an example, the method is used to determine the myoglobin concentration in individual skeletal muscle fibers and in control and hypertrophied rat cardiomyocytes. The amount of myoglobin per cardiomyocyte nucleus is increased two- to threefold in hypertrophied cardiomyocytes, whereas changes in myoglobin concentration depend on the model of hypertrophy used.
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Cardiomyocytes contain about 300 μM Mb.
“Cardiomyocytes contain about 300 μM Mb [57].”
From Mechanisms of nitrite bioactivation (2014) · cites this paper as [57]
Cites
A simple analysis of the "phosphocreatine shuttle"
Meyer, R. A., Sweeney, H. L., & Kushmerick, M. J. (1984) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1984.246.5.c365 · Cited by 523 · see citing works
Wittenberg, B. A., & Wittenberg, J. B. (1989) · Annual Review of Physiology · doi:10.1146/annurev.ph.51.030189.004233 · Cited by 468 · see citing works
Myocardial infarction in rats. Infarct size, myocyte hypertrophy, and capillary growth.
Anversa, P., Beghi, C., Kikkawa, Y., & Olivetti, G. (1986) · Circulation Research · doi:10.1161/01.res.58.1.26 · Cited by 279 · see citing works
An assay for sarcoplasmic reticulum Ca2+-ATPase activity in muscle homogenates
Simonides, W. S., & van Hardeveld, C. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90226-y · Cited by 173 · see citing works
Morphometric analysis of biologic processes.
Loud, A. V., & Anversa, P. (1984) · PubMed · Cited by 118 · see citing works
van der Laarse, W. J., Diegenbach, P. C., & Elzinga, G. (1989) · Journal of Muscle Research and Cell Motility · doi:10.1007/bf01739812 · Cited by 95 · see citing works
Self-citationMyoglobin levels in individual human skeletal muscle fibers of different types.
Nemeth, P. M., & Lowry, O. H. (1984) · Journal of Histochemistry & Cytochemistry · doi:10.1177/32.11.6491255 · Cited by 74 · see citing works
O'Brien, P. J., Shen, H. M., McCutcheon, L. J., O’Grady, M. R., Byrne, P., Ferguson, H. W., Mirsalimi, M., Julian, R. J., Sargeant, J., Tremblay, R. M., et al. (1992) · Molecular and Cellular Biochemistry · doi:10.1007/bf00229642 · Cited by 68 · see citing works
Quantitative succinate-dehydrogenase histochemistry
Pool, C. W., Diegenbach, P. C., & Scholten, G. (1979) · Histochemistry and Cell Biology · doi:10.1007/bf00495025 · Cited by 59 · see citing works
Postnatal development and plasticity of specialized muscle fiber characteristics in the hindlimb
Garry, D. J., Bassel‐Duby, R., Richardson, J. A., Grayson, J. M., Neufer, P. D., & Williams, R. S. (1996) · Developmental Genetics · doi:10.1002/(sici)1520-6408(1996)19:2 · Cited by 58 · see citing works
On the role of myoglobin in muscle respiration
Murray, J. D. (1974) · Journal of Theoretical Biology · doi:10.1016/0022-5193(74)90102-7 · Cited by 57 · see citing works
Nibbering, P. H., Marijnen, J. G. J., Rapp, A. K., Leijh, P. C. J., & van Furth, R. (1986) · Histochemistry and Cell Biology · doi:10.1007/bf00482988 · Cited by 45 · see citing works
Myocardial myoglobin deficiency in various animal models of congestive heart failure
O'Brien, P. J. (1992) · Journal of Molecular and Cellular Cardiology · doi:10.1016/0022-2828(92)93386-x · Cited by 44 · see citing works
Reduction of Myocardial Myoglobin in Bovine Dilated Cardiomyopathy
Weil, J., Thomas Eschenhagen, A., Magnussen, O., Mittmann, C., Orthey, E., Scholz, H., Schäfer, H., & Scholtysik, G. (1997) · Journal of Molecular and Cellular Cardiology · doi:10.1006/jmcc.1996.0318 · Cited by 33 · see citing works
Zuurbier, C. J., & van Beek, H. (1997) · Circulation Research · doi:10.1161/01.res.81.1.69 · Cited by 30 · see citing works
O'Brien, P. J., Duke, A. L., Shen, H. M., & Shohet, R. V. (1995) · Molecular and Cellular Biochemistry · doi:10.1007/bf00928935 · Cited by 22 · see citing works
The radial distribution of succinate dehydrogenase activity in porcine muscle fibres
Swatland, H. J. (1984) · The Histochemical Journal · doi:10.1007/bf01003615 · Cited by 15 · see citing works
van der Laarse, W. J., Maslam, S., & Diegenbach, P. C. (1985) · The Histochemical Journal · doi:10.1007/bf01003398 · Cited by 12 · see citing works
Self-citationChanges in ventricular 1,2-diacylglycerol content in rats following monocrotaline treatment
Okumura, K., Kondo, J., Shimizu, K., Yoshino, M., Toki, Y., Hashimoto, H., & Ito, T. (1992) · Cardiovascular Research · doi:10.1093/cvr/26.6.626 · Cited by 6 · see citing works
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Mechanisms of nitrite bioactivation
Kim-Shapiro, D. B., & Gladwin, M. T. (2014) · Nitric Oxide · doi:10.1016/j.niox.2013.11.002 · Cited by 157 · see citing works
The impact of biochemical methods for single muscle fibre analysis
Pette, D., Peuker, H., & Staron, R. S. (1999) · Acta Physiologica Scandinavica · doi:10.1046/j.1365-201x.1999.00573.x · Cited by 128 · see citing works
Antioxidant treatment attenuates pulmonary arterial hypertension-induced heart failure
Redout, E. M., van der Toorn, A., Zuidwijk, M. J., van de Kolk, C. W. A., van Echteld, C. J. A., Musters, R. J. P., van Hardeveld, C., Paulus, W. J., & Simonides, W. S. (2010) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00097.2009 · Cited by 117 · see citing works
Ruiter, G., Manders, E., Happé, C. M., Schalij, I., Groepenhoff, H., Howard, L., Wilkins, M. R., Bogaard, H. J., Westerhof, N., van der Laarse, W. J., et al. (2015) · Pulmonary Circulation · doi:10.1086/682217 · Cited by 108 · see citing works
Self-citationvan der Zwaard, S., de Ruiter, C. J., Noordhof, D. A., Sterrenburg, R., Bloemers, F. W., de Koning, J. J., Jaspers, R. T., & van der Laarse, W. J. (2016) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00355.2016 · Cited by 100 · see citing works
Self-citationUnder the Hood: Skeletal Muscle Determinants of Endurance Performance
van der Zwaard, S., Brocherie, F., & Jaspers, R. T. (2021) · Frontiers in Sports and Active Living · doi:10.3389/fspor.2021.719434 · Cited by 91 · see citing works
van der Zwaard, S., van der Laarse, W. J., Weide, G., Bloemers, F. W., Hofmijster, M. J., Levels, K., Noordhof, D. A., de Koning, J. J., de Ruiter, C. J., & Jaspers, R. T. (2017) · The FASEB Journal · doi:10.1096/fj.201700827r · Cited by 79 · see citing works
Self-citationvan Weel, V., Deckers, M. M. L., Grimbergen, J. M., van Leuven, K., Lardenoye, J.-W. H. P., Schlingemann, R. O., Amerongen, G. P. V. N., van Bockel, J. H., van Hinsbergh, V. W. M., & Quax, P. H. A. (2004) · Circulation Research · doi:10.1161/01.res.0000133247.69803.c3 · Cited by 73 · see citing works
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Tombe, A. L. D., van Beek-Harmsen, B. J., Groot, M. B. E. L.-D., & van der Laarse, W. J. (2002) · Microscopy Research and Technique · doi:10.1002/jemt.10153 · Cited by 67 · see citing works
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Self-citationvan der Laarse, W. J., Tombe, A. L. D., van Beek-Harmsen, B. J., Groot, M. B. E. L.-D., & Jaspers, R. T. (2005) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00470.2005 · Cited by 44 · see citing works
Self-citationImmunohistochemical Determination of Cytosolic Cytochrome c Concentration in Cardiomyocytes
van Beek-Harmsen, B. J., & van der Laarse, W. J. (2005) · Journal of Histochemistry & Cytochemistry · doi:10.1369/jhc.4c6527.2005 · Cited by 42 · see citing works
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Duncker, D. J., Boontje, N. M., Merkus, D., Versteilen, A. M. G., Krysiak, J., Mearini, G., El‐Armouche, A., de Beer, V. J., Lamers, J. M. J., Carrier, L., et al. (2009) · Circulation Heart Failure · doi:10.1161/circheartfailure.108.806125 · Cited by 40 · see citing works
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Bekedam, M. A., van Beek-Harmsen, B. J., van Mechelen, W., Boonstra, A., & van der Laarse, W. J. (2009) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00149.2009 · Cited by 37 · see citing works
Self-citationvan der Meer, S. F. T., Jaspers, R. T., Jones, D. A., & Degens, H. (2010) · Annals of Anatomy - Anatomischer Anzeiger · doi:10.1016/j.aanat.2010.08.004 · Cited by 35 · see citing works
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Jaspers, R. T., Testerink, J., Gaspera, B. D., Chanoine, C., Bagowski, C. P., & van der Laarse, W. J. (2014) · Biology Open · doi:10.1242/bio.20149167 · Cited by 29 · see citing works
Self-citationGussakovsky, E. E., Jilkina, O., Yang, Y., & Kupriyanov, V. V. (2008) · Analytical Biochemistry · doi:10.1016/j.ab.2008.07.028 · Cited by 20 · see citing works
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Kerkhof, C. J. M., van der Linden, P. J. W., & Sipkema, P. (2002) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00179.2001 · Cited by 19 · see citing works
Peters, E. L., der Linde, S. V., Vogel, I. S. P., Haroon, M. F., Offringa, C., de Wit, G. M. J., Koolwijk, P., van der Laarse, W. J., & Jaspers, R. T. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18091889 · Cited by 18 · see citing works
Self-citationSmeding, L. S., van der Laarse, W. J., van Veelen, T. A., Lamberts, R. R., Niessen, H. W. M., Kneyber, M. C. J., Groeneveld, A. B. J., & Plötz, F. B. (2011) · Journal of Surgical Research · doi:10.1016/j.jss.2011.05.055 · Cited by 17 · see citing works
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Self-citationBekedam, M. A., van Beek-Harmsen, B. J., van Mechelen, W., Boonstra, A., Visser, F. C., & van der Laarse, W. J. (2008) · International Journal of Cardiology · doi:10.1016/j.ijcard.2007.12.025 · Cited by 14 · see citing works
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de Beer, E. L., Bottone, A. E., van Rijk, M. C., van der Velden, J., & Voest, E. E. (2002) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0704621 · Cited by 13 · see citing works
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de Groot, B., Zuurbier, C. J., & van Beek, H. (1999) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.1999.276.5.h1616 · Cited by 11 · see citing works
Bouwman, R. A., Hof, F. N. G. V. ’., de Ruijter, W., van Beek-Harmsen, B. J., Musters, R. J. P., de Lange, J. J., & Boer, C. (2006) · British Journal of Anaesthesia · doi:10.1093/bja/ael174 · Cited by 9 · see citing works
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- Authors
- B.E., M., Groot, L.-D., Tombe, A. L. D., & Laarse, W. J. V. D.
- Affiliations
- Amsterdam Cardiovascular Sciences (NL)
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1998-09
- Volume
- 46
- Issue
- 9
- Pages
- 1077-1084
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2152179828
- Cited by
- 39
- References
- 19
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 1 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 1 · 2014: 2 · 2013: 2 · 2012: 2
- FWCI
- 0.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Histochemistry & Cytochemistry (publishedVersion, public-domain)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiomyopathy and Myosin Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hemoglobin structure and function
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Fibrosis and Remodeling
- Keywords
- cardiomyocytes, microdensitometry, skeletal muscle fibers, cardiomyocyte hypertrophy, rat heart
- MeSH terms
- Animals, Calibration, Histocytochemistry, Image Processing, Computer-Assisted, Male, Myocardium, Myoglobin, Rabbits, Succinate Dehydrogenase, Rats, Wistar, Muscle, Skeletal, Rats
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