Melatonin, quercetin and resveratrol attenuates oxidative hepatocellular injury in streptozotocin-induced diabetic rats
Abstract
In this study, effects of melatonin, quercetin and resveratrol on hepatocellular injury in streptozotocin (STZ)-induced experimental diabetes were aimed to be investigated by histological and biochemical methods. Thirty-five male Wistar albino rats were divided into five groups, namely, control, diabetes (STZ 45 mg/kg/single dose/intraperitoneally (ip)), diabetes + melatonin (10 mg/kg/30 days/ip), diabetes + quercetin (25 mg/kg/30 days/ip) and diabetes + resveratrol (10 mg/kg/30 days/ip). Initial and final blood glucose levels and body weights (BWs) were measured. At the end of the experimentation, following routine tissue processing procedure, sections were stained with haematoxylin-eosin (H-E), periodic acid Schiff and Masson's trichrome. Tissue malondialdehyde (MDA) and glutathione (GSH) levels and superoxide dismutase (SOD) and catalase (CAT) activities were examined. The diabetic rats had significantly higher blood glucose levels than those of control rats (p = 0.0001). Mean BWs of diabetic rats were significantly decreased when compared with the control rats (p = 0.0013). Histopathological alterations including cellular glycogen depletion, congestion, sinusoidal dilatation, inflammation and fibrosis were detected in diabetes group. On the other hand, histopathological changes markedly reduced in all of the treatment groups (p = 0.001). Mean tissue MDA level was increased but mean tissue CAT and SOD activities and GSH levels were decreased in the diabetes group. Melatonin, quercetin and resveratrol administered diabetic rats showed an increase in CAT activities and GSH levels and a decrease in MDA levels (p < 0.05, for all). Melatonin, quercetin and resveratrol administrations markedly reduced hepatocellular injury in STZ-induced experimental diabetes.
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There are initial indications that the antioxidant and anti-inflammatory actions of melatonin may work together with select polyphenols (e.g., resveratrol, epigallocatechin 3-gallate) for therapeutic indications.
“There are initial indications that the antioxidant and anti-inflammatory actions of melatonin may work together with select polyphenols (e.g., resveratrol, epigallocatechin 3-gallate) for therapeutic indications [48,49,50,51,52,53,54].”
From Is Melatonin the “Next Vitamin D”?: A Review of Emerging Science, Clinical Uses, Safety, and Dietary Supplements (2022) · cites this paper as [52]
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Minich, D. M., Henning, M., Darley, C., Fahoum, M., Schuler, C. B., & Frame, J. (2022) · Nutrients · doi:10.3390/nu14193934 · Cited by 176 · see citing works
- Authors
- Elbe, H., Esrefoglu, M., Vardi, N., Taslidere, E., Ozerol, E., & Tanbek, K.
- Affiliations
- Muğla University (TR)
- Department of Histology and Embryology, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey
- Department of Histology and Embryology, Faculty of Medicine, Inonu University, Malatya, Turkey
- Department of Biochemistry, Faculty of Medicine, Inonu University, Malatya, Turkey
- Inonu University (TR)
- Corresponding author
- Elbe, H
- Venue
- Human & Experimental Toxicology
- Publisher
- SAGE Publications
- Published
- 2015-08-18
- Volume
- 34
- Issue
- 9
- Pages
- 859-868
- ISSN
- 0960-3271 (ISSN-L)
- 1477-0903
- OpenAlex
- W1804033261
- Cited by
- 37
- References
- 55
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 3 · 2020: 4 · 2019: 2 · 2018: 7 · 2017: 8 · 2016: 3 · 2015: 1
- FWCI
- 5.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Human & Experimental Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Keywords
- melatonin, quercetin, resveratrol, hepatocyte injury, streptozotocin-induced diabetes, oxidative stress, liver histopathology, malondialdehyde, glutathione, superoxide dismutase, catalase, antioxidant defense
- MeSH terms
- Resveratrol, Animals, Antioxidants, Blood Glucose, Body Weight, Catalase, Diabetes Mellitus, Experimental, Hepatitis, Male, Melatonin, Quercetin, Stilbenes, Superoxide Dismutase, Biomarkers, Rats, Wistar, Oxidative Stress, Hepatocytes, Diabetes Complications, Rats
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