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Cited by 87Biochemical and Structural CharacterizationBacterial Genetics and BiotechnologyEscherichia coli research studies

Molecular Strategies for Fimbrial Expression and Assembly

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Hui Wu, Fives-Taylor, Paula M.·2001·Critical Reviews in Oral Biology & Medicine
DOI 10.1177/10454411010120020101
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Abstract

Fimbriae or pili are long, filamentous, multimeric macromolecules found on the bacterial cell surface. Bacteria express a diverse array of fimbriae or pili that are involved in bacterial adherence and invasion. Fimbriae can be categorized based on their modes of expression and assembly. Type I fimbriae and P pili are distributed peritrichously and translocated to the cell surface by a chaperone/usher pathway. Type 4 pili are located at the pole of the cell and assembled via the type II secretion system. Curli fimbriae are coiled surface structures assembled by an extracellular nucleation/precipitation pathway. Fimbriae of oral Gram-negative and Gram-positive bacteria have not been well-studied as compared with the fimbriae of enteric pathogens. Oral pathogens, such as Eikenella corrodens, Actinobacillus actinomycetemcomitans, and Porphyromonas gingivalis, possess fimbriae that have been implicated in bacterial adhesion and invasion. These fimbriae are potential virulence factors in oral infectious processes. A. actinomycetemcomitans and E. corrodens have Type 4-like fimbriae, whereas P. gingivalis displays a unique type of fimbriae. To date, fimbriae of the oral primary colonizers, Actinomyces naeslundii and Streptococcus parasanguis, represent the only fimbriae characterized for any Gram-positive bacteria. The putative major fimbrial subunits, FimA and FimP of A. naeslundii and Fapl of S. parasanguis, contain a signal sequence and cell-wall-sorting signal. The presence of extensive dipeptide repeats in Fapl makes it unique among fimbrial molecules. Based on experimental data, a nucleation/precipitation pathway is proposed for fimbrial biogenesis of both S. parasanguis and A. naeslundii, although we cannot rule out an alternative covalent linkage model. The model systems described in this review served as a framework for hypotheses for how the known molecular factors of fimbriae on oral bacteria may be expressed and assembled.

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  • Orf4 is responsible for assembly of type I pili but not type II fimbriae, whereas Orf365 fulfills the reciprocal role for type II fimbriae.

    “Orf4 appears to be responsible for the assembly of type I pili but not of type II fimbriae, whereas Orf365 fulfills the reciprocal role for type II fimbriae [3,58].”

    From Protein sorting to the cell wall envelope of Gram-positive bacteria (2004) · cites this paper as [3]

    The discussion of A. naeslundii fimbriae (p. 107) identifies Orf4 among genes involved in type I assembly and Orf365 as a type II assembly gene. It does not establish that their roles are exclusive: it suggests some fimbrial genes may be interchangeable and that Orf365 may also process a type I subunit. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • S. parasanguis assembles adhesive filaments containing Fap1, which harbors a cell wall sorting signal.

    “S. parasanguis assembles adhesive filaments containing Fap1, which harbors a cell wall sorting signal.”

    From Protein sorting to the cell wall envelope of Gram-positive bacteria (2004) · cites this paper as [3]

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Research works printed but not linked: 6

  1. [24]24. Davies MI (1992). The pathology of cardiac valves. In: Oxford text- book of pathology. McGee 10, Isaacson PG, Wright NA, editors. New York: Oxford University Press, pp. 875-878.
  2. [29]29. Ellen R (1982). Oral colonization by Gram-positive bacteria signifi- cant to periodontal disease. In: Host parasite interactions in periodontal diseases. Genco RJ, Mergenhagen SE, editors. Washington, DC: American Society for Microbiology, pp. 98-11 1.
  3. [30]30. Fachon S, Fives-Taylor P (1978). Surface structures of Streptococcus sanguis and Streptococcus salivarius (abstract). In: Abstracts of Annual Meeting. Washington, DC: American Society for Microbiology, p.77. Fachon-Kalweit S (1985). Physiochemical characterization of fim- briae and other surface antigens from Streptococcus parasanguis FW2 13 (PhD thesis). The University of Vermont: Burlington, p. 134.
  4. [38]38. Schlesinger D, editor. Washington, DC: American Society for Microbiology, pp. 206-209.
  5. [129]129. Thanassi DG, Saulino ET, Lombardo MI, Roth R, Heuser I, 114 Crit Rev Oral Biol Med 12(2):l0l 1 14 Crit Rev Oral Biol Med 12(2):101-115 (2001)
  6. [141]141. Wu H (1999). Identification and characterization of the fapl locus of Streptococcus parasanguis FW213 encoding a major fimbrial adhesin (PhD thesis). University of Vermont: Cell and Molecular Biology Program, Department of Microbiology and Molecular Genetics, Burlington, p. 190.

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Authors
Wu, H., & Fives-Taylor, P. M.
Affiliations
University of Vermont (US)
ORCID
Hui Wu
Venue
Critical Reviews in Oral Biology & Medicine
Publisher
SAGE Publications
Published
2001-03
Volume
12
Issue
2
Pages
101-115
ISSN
  • 1045-4411 (ISSN-L)
  • 1544-1113
DOI
10.1177/10454411010120020101
OpenAlex
W2095678870
Cited by
87
References
144
Citations by year
2026: 3 · 2024: 2 · 2023: 3 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 4 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 3 · 2012: 3
FWCI
0.32
Type
journal article
Language
en
Open access
Licence
journals.sagepub.com/page/policies/text-and-data-mining-license (tdm)
Locations
  • Critical Reviews in Oral Biology & Medicine (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Fondazione Italiana per la ricerca sulle Malattie del Pancreas
  • National Institutes of Health
  • National Institute of Dental and Craniofacial Research (R01DE011000, R37-DE1100, R01 DE09760, R01DE009760)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
Keywords
bacterial fimbriae, fimbriae, chaperone-usher pathway, type II secretion system, curli fimbriae, oral bacteria, Actinomyces naeslundii, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Porphyromonas gingivalis, fimA, FIMP, bacterial adhesion, virulence factors
MeSH terms
Actinomyces, Bacterial Adhesion, Genes, Bacterial, Fimbriae, Bacterial, Streptococcus, Signal Transduction, Gene Expression Regulation, Bacterial, Eikenella corrodens, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans

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