New Serum Biomarkers for Detection of Endometriosis Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
Open accessAbstract
This study used proteomic fingerprint technology, combining nano-sized magnetic beads with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI—TOF—MS), to screen for potential protein biomarkers for the diagnosis of endometriosis. Serum proteins from 126 patients with endometriosis and 120 healthy controls were profiled and compared. Biomarker pattern software identified 46 discriminating mass-to-charge m/z ratio peaks that were related to endometriosis. The model constructed by the software, based on three of these peaks ( m/z 5988.7, 7185.3 and 8929.8), generated excellent separation between the endometriosis and control groups. The sensitivity was 91.4% and the specificity 95.0%. Blind testing on a second series of serum samples from patients with endometriosis and healthy controls indicated a sensitivity of 89.3% and a specificity of 90.0%. Biomarkers for endometriosis can be discovered in serum by MALDI—TOF—MS in combination with nano-sized magnetic beads. The pattern of combined markers provides a powerful and reliable diagnostic method for endometriosis, with high sensitivity and specificity.
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A proteomic fingerprint model based on three peptide peaks had 91.4% sensitivity and 95% specificity to detect endometriosis when comparing 126 patients with endometriosis with 120 healthy controls.
“A proteomic fingerprint model, based on three peptide peaks, had 91.4% sensitivity and 95% specificity to detect endometriosis when comparing 126 patients with endometriosis with 120 healthy controls [90].”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [90]
This combination of peptide peaks was validated in an independent cohort, showing sensitivity of 89.3% and specificity of 90%.
“Furthermore, this combination of peptide peaks was validated in an independent cohort, showing a sensitivity of 89.3% and a specificity of 90% [90].”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [90]
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- Authors
- Zheng, N., Pan, C., & Liu, W.
- Affiliations
- Tongde Hospital of Zhejiang Province (CN)
- Taizhou Municipal Hospital (CN)
- Corresponding author
- Pan, C
- ORCID
- Zheng, N
- Venue
- Journal of International Medical Research
- Publisher
- SAGE Publications
- Published
- 2011-08
- Volume
- 39
- Issue
- 4
- Pages
- 1184-1192
- ISSN
- 0300-0605 (ISSN-L)
- 1473-2300
- OpenAlex
- W2317078460
- Cited by
- 28
- References
- 28
- Citations by year
- 2025: 1 · 2024: 1 · 2022: 3 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 2
- FWCI
- 2.14
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of International Medical Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Keywords
- endometriosis, serum biomarkers, MALDI-TOF mass spectrometry, serum proteomics, protein biomarkers, endometriosis diagnosis, sensitivity and specificity
- MeSH terms
- Adult, Blood Proteins, Endometriosis, Female, Humans, Prognosis, Sensitivity and Specificity, Biomarkers, Tumor, Biomarkers, Case-Control Studies, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Proteomics
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