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Cited by 41Platelet Disorders and TreatmentsT-cell and B-cell ImmunologyImmunotherapy and Immune Responses

Regulation of Th1/Th2 and Th17/Treg by pDC/mDC imbalance in primary immune thrombocytopenia.

Open access
Li, Qinzhi, Liu, Yang, Wang, Xiujuan, Sun, Mingling, Wang, Lei, Wang, Xinyou, Liu, Ying, Fan, Wenxia +3 more·2021·Experimental Biology and Medicine
DOI 10.1177/15353702211009787PMC PMC8719040
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Abstract

This study investigates the regulatory effect of plasmacytoid dendritic cells (pDC)/myeloid dendritic cells (mDC) imbalance on balance of Th1/Th2 and Th17/Treg in primary immune thrombocytopenia (ITP). A total of 30 untreated ITP patients and 20 healthy controls were recruited. Compared with healthy control, the pDC proportion of ITP patients was significantly reduced ( P = 0.004), while the mDC proportion was not significantly changed ( P = 0.681), resulting in a decrease in the pDC/mDC ratio ( P = 0.001). Additionally, compared with controls, serum levels of interleukin (IL)-6, IL-12, and IL-23 were increased in ITP patients ( P < 0.001), and mRNA levels of IL-12p40, IL-12p35, and IL-23p19 were also increased ( P =0.014, P = 0.043, P < 0.001). Compared with the healthy control, the proportion of Th1 and Th17 cells in ITP patients increased ( P = 0.001, P = 0.031). Serum levels of interferon gamma (IFN-γ) and IL-17 in ITP patients also increased ( P = 0.025, P = 0.005). Furthermore, T-bet and RORγt mRNA levels were increased in peripheral blood of ITP patients ( P = 0.018, P < 0.001). Correspondingly, the proportion of Th2 and Treg cells decreased ( P = 0.007, P < 0.001), along with a decrease in serum IL-4 and transforming growth factor beta (TGF-β) ( P = 0.028, P = 0.042), and an increase in GATA-3 mRNA ( P < 0.001). However, there was no significant difference in Foxp3 mRNA levels ( P = 0.587). Pearson correlation analysis showed that the proportion of total dendritic cells (DCs) was positively correlated with IL-12 ( r = 0.526, P = 0.003) and IL-23 ( r = 0.501, P = 0.005) in ITP patients. Th1/Th2 ratio, IFN-γ, and IL-12 levels were negatively correlated with platelet counts ( r = −0.494, P = 0.009; r = –0.415, P = 0.028; r = –0.492, P = 0.032). However, IL-23 was positively correlated with IL-17 ( r = 0.489, P = 0.006) and negatively correlated with platelet count ( r = –0.564, P = 0.001). The ratio of IL-6 and Th17 cells was negatively correlated with platelet count ( r = –0.443, P = 0.014; r = –0.471, P = 0.011). The imbalance of pDC/mDC and the increase of IL-6, IL-12, and IL-23 lead to the increased differentiation of CD4+ T cells into Th1 and Th17 cells, which might be the important mechanisms underlying the imbalance of Th1/Th2 and Th17/Treg in ITP patients.

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Authors
Li, Q., Liu, Y., Wang, X., Sun, M., Wang, L., Wang, X., Liu, Y., Fan, W., Zhang, K., Sui, X., & Guo, X.
Affiliations
Xinjiang Medical University (CN)
ORCID
Venue
Experimental Biology and Medicine
Publisher
Frontiers Media SA
Published
2021-05-02
Volume
246
Issue
15
Pages
1688-1697
ISSN
  • 0071-3384 (ISSN-L)
  • 1535-3702
  • 1535-3699
DOI
10.1177/15353702211009787
PMC
PMC8719040
OpenAlex
W3158187035
Cited by
41
References
35
Citations by year
2026: 4 · 2025: 11 · 2024: 10 · 2023: 7 · 2022: 8 · 2021: 1
FWCI
4.24
Type
journal article
Language
en
Open access
Licence
journals.sagepub.com/page/policies/text-and-data-mining-license (tdm)
Locations
  • Experimental Biology and Medicine (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Natural Science Foundation of China (81360086)
Topics
  • Health Sciences › Medicine › Hematology › Platelet Disorders and Treatments
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
Keywords
primary immune thrombocytopenia, plasmacytoid dendritic cells, myeloid dendritic cells, Th1/Th2 balance, Th17/Treg balance, IL-12, IL-23, interleukin-6, interferon-gamma, IL-17, T-bet, RORγt, GATA3, Foxp3
MeSH terms
Adult, Dendritic Cells, Female, Humans, Leukocytes, Mononuclear, Male, Middle Aged, Transforming Growth Factor beta, Purpura, Thrombocytopenic, Idiopathic, Interleukin-17, T-Lymphocytes, Regulatory, Th17 Cells

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