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Development and characterisation of a low-concentration sodium dodecyl sulphate decellularised porcine dermis
Open accessAbstract
The aim of this study was to adapt a proprietary decellularisation process for human dermis for use with porcine skin. Porcine skin was subject to: sodium chloride (1 M) to detach the epidermis, trypsin paste to remove hair follicles, peracetic acid (0.1% v/v) disinfection, washed in hypotonic buffer and 0.1% (w/v) sodium dodecyl sulphate in the presence of proteinase inhibitors followed by nuclease treatment. Cellular porcine skin, decellularised porcine and human dermis were compared using histology, immunohistochemistry, GSL-1 lectin (alpha-gal epitope) staining, biochemical assays, uniaxial tensile and in vitro cytotoxicity tests. There was no microscopic evidence of cells in decellularised porcine dermis. DNA content was reduced by 98.2% compared to cellular porcine skin. There were no significant differences in the biomechanical parameters studied or evidence of cytotoxicity. The decellularised porcine dermis retained residual alpha-gal epitope. Basement membrane collagen IV immunostaining was lost following decellularisation; however, laminin staining was retained.
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There are contradictory data about the degenerative effects of remaining α-gal epitopes within decellularized tissues.
“However, there are contradictory data about the degenerative effects of remaining α-gal epitopes within decellularized tissues [53, 122, 123].”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [123]
The Discussion (page unavailable) reports residual α-gal in decellularised porcine dermis and says its effect on the host response is unclear. It cites evidence suggesting no adverse effect on remodelling of decellularised tissue, but does not describe conflicting evidence of degenerative effects within decellularised tissues; the rejection example concerns cellular xenografts. (LLM, conf=0.84)
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Research works printed but not linked: 28
- Palfreyman S, 2008, Nurs Times, 104, 34
- 10.1089/wound.2014.0604 · DOI
- 10.1007/s10561-012-9333-1 · DOI
- 10.1016/j.biomaterials.2006.10.005 · DOI
- 10.1089/tea.2007.0233 · DOI
- 10.1002/jbm.a.33171 · DOI
- 10.1111/wrr.12113 · DOI
- 10.1371/journal.pone.0113209 · DOI
- 10.1007/s10561-014-9479-0 · DOI
- 10.1007/s10856-015-5517-0 · DOI
- 10.1016/j.biomaterials.2011.01.057 · DOI · Paper page
- 10.1098/rsif.2009.0403 · DOI
- Wilcox HE, 2005, J Heart Valve Dis, 14, 228
- Korossis SA, 2005, J Heart Valve Dis, 14, 408
- 10.1016/j.biomaterials.2010.08.005 · DOI
- 10.1016/j.ymeth.2015.03.005 · DOI · Paper page
- 10.1016/j.smim.2007.11.003 · DOI · Paper page
- 10.1111/j.1432-2277.2005.00124.x · DOI
- 10.1155/2015/589648 · DOI
- Lonne-Rahm S, 2012, J Clin Exp Dermatol Res, 3, 156
- 10.1111/j.0909-752X.2006.00146.x · DOI
- 10.1111/j.1365-2133.1987.tb04921.x · DOI
- 10.1089/ten.2006.12.2117 · DOI
- 10.1089/ten.tea.2011.0287 · DOI
- 10.1007/978-1-4612-0557-9 · DOI
- Gallagher A, Proceedings of the IRCOBI conference 2012
- 10.1016/j.biomaterials.2003.09.070 · DOI
- 10.1002/jbm.b.30938 · DOI
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Kasravi, M., Ahmadi, A., Babajani, A., Mazloomnejad, R., Hatamnejad, M. R., Shariatzadeh, S., Bahrami, S., & Niknejad, H. (2023) · Biomaterials Research · doi:10.1186/s40824-023-00348-z · Cited by 316
Metadata
- Authors
- Helliwell, J. A., Thomas, D. S., Papathanasiou, V., Homer-Vanniasinkam, S., Desai, A., Jennings, L. M., Rooney, P., Kearney, J. N., & Ingham, E.
- Affiliations
- University of Leeds (GB)
- Leeds General Infirmary (GB)
- Leeds Vascular Institute, Leeds General Infirmary, Leeds, UK
- NHS Blood and Transplant (GB)
- Corresponding author
- Ingham, Eileen
- Venue
- Journal of Tissue Engineering
- Publisher
- SAGE Publications
- Published
- 2017-01-01
- Volume
- 8
- Pages
- 2041731417724011-2041731417724011
- ISSN
- 2041-7314 (ISSN-L)
- PMC
- PMC5546651
- OpenAlex
- W2743956150
- Cited by
- 21
- References
- 0
- Citations by year
- 2026: 4 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 4 · 2020: 2 · 2019: 4
- FWCI
- 1.68
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Journal of Tissue Engineering (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- Wellcome Trust (/WT088908/Z/09/Z)
- Engineering and Physical Sciences Research Council (EP/N00941X/1, EP/J021156/1, EP/J017620/1)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Keywords
- porcine skin, sodium dodecyl sulfate, alpha-gal epitope, laminin, nuclease treatment, protease inhibitors, skin biomechanics, cytotoxicity, tissue engineering scaffolds
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