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Colloidal gold, a useful marker for transmission and scanning electron microscopy.
Open accessAbstract
Electron dense markers of a size suitable for transmission electron microscopy and scanning electron microscopy have been prepared with gold granules labeled with a monolayer of specific macromolecules. The optimum conditions for preparing the markers have been ascertained. The method is simple, rapid and seems to be general since gold granules have been labeled with polysaccharides and proteins. As homogeneous populations of gold granules having different sizes can be prepared, the method is also suitable for double marking experiments. The gold technique is illustrated by the localization of polysaccharides and glycoproteins on yeast cell walls and erythrocyte membranes by transmission electron microscopy and on yeast cells and intact erythrocytes by scanning electron microscopy. Good spatial resolution of the marker was achieved in all cases. The method is also suitable for marking thin sections. Spectrophotometric measurements were used to determine the number of gold granules adsorbed per cell.
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Cited by
Stoscheck, C. M. (1990) · Methods in Enzymology · doi:10.1016/0076-6879(90)82008-p · Cited by 1,438
Kolb-Bachofen, V., Schlepper-Schäfer, J., Vogell, W., & Kolb, H. (1982) · Cell · doi:10.1016/0092-8674(82)90447-0 · Cited by 145
Schüpbach, P., Neeser, J. R., Golliard, M., Rouvet, M., & Guggenheim, B. (1996) · Journal of Dental Research · doi:10.1177/00220345960750101101 · Cited by 126
Ultrastructural Cytochemistry of Blood-Brain Barrier Endothelia
Vorbrodt, A. W. (1988) · Progress in Histochemistry and Cytochemistry · doi:10.1016/s0079-6336(88)80001-9 · Cited by 108
MRI of whole rat brain perivascular network reveals role for ventricles in brain waste clearance
Magdoom, K. N., Brown, A., Rey, J., Mareci, T. H., King, M. A., & Sarntinoranont, M. (2019) · Scientific Reports · doi:10.1038/s41598-019-44938-1 · Cited by 35
Metadata
- Authors
- Horisberger, M., & Rosset, J.
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1977-04
- Volume
- 25
- Issue
- 4
- Pages
- 295-305
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2142874501
- Cited by
- 627
- References
- 0
- FWCI
- 10.51
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Horisberger, M., & Rosset, J. (1977). Colloidal gold, a useful marker for transmission and scanning electron microscopy. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1177/25.4.323352
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
