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Cited by 35Cerebrospinal fluid and hydrocephalusAdvanced MRI Techniques and ApplicationsBarrier Structure and Function Studies

MRI of whole rat brain perivascular network reveals role for ventricles in brain waste clearance

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Magdoom, Kulam Najmudeen, Brown, Alec, Rey, Julian, Mareci, Thomas H., King, Michael A., Sarntinoranont, Malisa·2019·Scientific Reports
DOI 10.1038/s41598-019-44938-1PMC PMC6685961
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Abstract

Investigating the mechanisms by which metabolic wastes are cleared from nervous tissue is important for understanding natural function and the pathophysiology of several neurological disorders including Alzheimer’s disease. Recent evidence suggests clearance may be the function of annular spaces around cerebral blood vessels, called perivascular spaces (PVS), through which cerebrospinal fluid (CSF) is transported from the subarachnoid space into brain parenchyma to exchange with interstitial fluid (also known as the glymphatic system). In this work, an MRI-based methodology was developed to reconstruct the PVS network in whole rat brain to better elucidate both PVS uptake and clearance pathways. MR visible tracer (Gd-albumin) was infused in vivo into the CSF-filled lateral ventricle followed by ex vivo high-resolution MR imaging at 17.6 T with an image voxel volume two orders of magnitude smaller than previously reported. Imaged tracer distribution patterns were reconstructed to obtain a more complete brain PVS network. Several PVS connections were repeatedly highlighted across different animals, and new PVS connections between ventricles and different parts of the brain parenchyma were revealed suggesting a possible role for the ventricles as a source or sink for solutes in the brain. In the future, this methodology may be applied to understand changes in the PVS network with disease.

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Sorted by citations

Authors
Magdoom, K. N., Brown, A., Rey, J., Mareci, T. H., King, M. A., & Sarntinoranont, M.
Affiliations
  • University of Florida (US)
  • Veterans Health Administration (US)
Corresponding author
Sarntinoranont, Malisa
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2019-08-07
Volume
9
Issue
1
Pages
11480-11480
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-019-44938-1
PMC
PMC6685961
OpenAlex
W2965530449
Cited by
35
References
9
Citations by year
2026: 3 · 2025: 7 · 2024: 4 · 2023: 5 · 2022: 6 · 2021: 3 · 2020: 5 · 2019: 2
FWCI
1.91
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Science Foundation (DMR-1157490)
  • U.S. Department of Health & Human Services | National Institutes of Health (1S10OD020026)
  • Chiari and Syringomyelia Foundation
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Cerebrospinal fluid and hydrocephalus
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
Keywords
perivascular spaces, glymphatic system, cerebrospinal fluid, brain waste clearance, lateral ventricle, rat brain, Alzheimer's disease, interstitial fluid
MeSH terms
Glymphatic System, Albumins, Alzheimer Disease, Animals, Cerebral Ventricles, Cerebrospinal Fluid, Contrast Media, Evans Blue, Feasibility Studies, Magnetic Resonance Imaging, Male, Subarachnoid Space, Gadolinium DTPA, Models, Animal, Rats, Infusions, Intraventricular

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