The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Analysis and sorting of living cells according to deoxyribonucleic acid content.
Abstract
The methods for measuring the deoxyribonucleic acid content of individual mammalian cells and sorting them on the basis of this parameter have until now required fixation or other treatment which renders the cells nonviable. Using a class of bis-benzimidazole dyes, Hoechst 33258 and 33342 and a multiparameter computer-controlled cell sorter, we have been able to stain and separate living cells in the G1, S, and G2+M phases of the cell cycle and to continue their growth in tissue culture with high retention of viability (greater than 90%) and no increase in heteroploidy. The quenching of the fluorescence of the bound dye by 5-bromodeoxyuridine incorporated into cellular deoxyribonucleic acid is being used with the flow system to detect and isolate mutants in deoxyribonucleic acid metabolism spectroscopically.
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Cites
Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.
Krishan, A. (1975) · The Journal of cell biology · doi:10.1083/jcb.66.1.188 · Cited by 1,728
Microfluorometric Detection of Deoxyribonucleic Acid Replication in Human Metaphase Chromosomes
Latt, S. A. (1973) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.70.12.3395 · Cited by 827
MATHEMATICAL ANALYSIS OF DNA DISTRIBUTIONS DERIVED FROM FLOW MICROFLUOROMETRY
Dean, P. N., & Jett, J. H. (1974) · The Journal of Cell Biology · doi:10.1083/jcb.60.2.523 · Cited by 791
Optical studies of the interaction of 33258 Hoechst with DNA, chromatin, and metaphase chromosomes
Latt, S. A., & Wohlleb, J. C. (1975) · Chromosoma · doi:10.1007/bf00364015 · Cited by 342
Staining of constitutive heterochromatin in mammalian chromosomes with a new fluorochrome
Hilwig, I., & Gropp, A. (1972) · Experimental Cell Research · doi:10.1016/0014-4827(72)90527-7 · Cited by 327
Rigler, R. W. (1966) · PubMed · Cited by 219
Chromosome measurement and sorting by flow systems.
Gray, J. W., Carrano, A. V., Steinmetz, L. L., Van Dilla, M. A., Moore, D. H., Mayall, B. H., & Mendelsohn, M. L. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.4.1231 · Cited by 192
Latt, S. A. (1974) · Journal of Histochemistry & Cytochemistry · doi:10.1177/22.7.478 · Cited by 69
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A side order of stem cells: the SP phenotype
Challen, G. A., & Little, M. H. (2006) · Stem Cells · doi:10.1634/stemcells.2005-0116 · Cited by 475
Simultaneous flow cytometric analysis of human T cell activation antigen expression and DNA content.
Cotner, T., Williams, J. M., Christenson, L., Shapiro, H. M., Strom, T. B., & Strominger, J. (1983) · The Journal of experimental medicine · doi:10.1084/jem.157.2.461 · Cited by 318
Förster, I., & Rajewsky, K. (1987) · European Journal of Immunology · doi:10.1002/eji.1830170414 · Cited by 313
Measurement of cell numbers in microtiter culture plates using the fluorescent dye Hoechst 33258
Papadimitriou, E., & Lelkes, P. I. (1993) · Journal of Immunological Methods · doi:10.1016/0022-1759(93)90405-v · Cited by 47
Menon, C., Bauer, T. W., Kelley, S. T., Raz, D. J., Bleier, J. I., Patel, K., Steele, K., Prabakaran, I., Shifrin, A., Buerk, D. G., et al. (2008) · International Journal of Cancer · doi:10.1002/ijc.23499 · Cited by 11
Metadata
- Authors
- Arndt-Jovin, D. J., & Jovin, T. M.
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1977-07
- Volume
- 25
- Issue
- 7
- Pages
- 585-589
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2030185565
- Cited by
- 383
- References
- 8
- FWCI
- 11.08
- Type
- journal article
- Language
- en
- Open access
- closed
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- Arndt-Jovin, D. J., & Jovin, T. M. (1977). Analysis and sorting of living cells according to deoxyribonucleic acid content. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1177/25.7.70450
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
