The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Autofluorescence of viable cultured mammalian cells.
Open accessAbstract
The autofluorescence other than intrinsic protein emission of viable cultured mammalian cells has been investigated. The fluorescence was found to originate in discrete cytoplasmic vesicle-like regions and to be absent from the nucleus. Excitation and emission spectra of viable cells revealed at least two distinct fluorescent species. Comparison of cell spectra with spectra of known cellular metabolites suggested that most, if not all, of the fluorescence arises from intracellular nicotinamide adenine dinucleotide (NADH) and riboflavin and flavin coenzymes. Various changes in culture conditions did not affect the observed autofluorescence intensity. A multiparameter flow system (MACCS) was used to compare the fluorescence intensities of numerous cultured mammalian cells.
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Cites
Histochemistry: Theoretical and Applied
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Reduced permeability in CHO cells as a mechanism of resistance to colchicine
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Localization and Kinetics of Reduced Pyridine Nucleotide in Living Cells by Microfluorometry
Chance, B., & Thorell, B. (1959) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)69722-4 · Cited by 254
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Welsher, K., Sherlock, S. P., & Dai, H. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1014501108 · Cited by 958
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Kann, O., & Kovács, R. (2007) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00222.2006 · Cited by 855
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Waters, J. C. (2009) · Journal of Cell Biology · doi:10.1083/jcb.200903097 · Cited by 613
Metadata
- Authors
- Aubin, J. E.
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1979-01
- Volume
- 27
- Issue
- 1
- Pages
- 36-43
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2097053416
- Cited by
- 555
- References
- 5
- FWCI
- 2.90
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Aubin, J. E. (1979). Autofluorescence of viable cultured mammalian cells. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1177/27.1.220325
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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