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Reduced permeability in CHO cells as a mechanism of resistance to colchicine
Abstract
Colchicine resistant (CH R) lines of stable phenotype have been isolated from cultured Chinese hamster (CHO) cells. Successive single‐step selections for increasing resistance were performed by isolating resistant colonies at each step. Two complementary assays involving [3 H] colchicine uptake by whole cells and binding of [3 H] colchicine by cytoplasmic extracts were developed to test for altered permeability and altered intracellular target protein, respectively. All clones isolated appeared to have decreased permeability to the drug while their colchicine‐binding ability was not reduced. The amount of reduction in colchicine uptake correlated strongly with cellular resistance. The CH R lines were also cross resistant to other drugs such as actinomycin D, vinblastine and Colcemid; furthermore, the degree of cross resistance was positively correlated with the degree of colchicine resistance. The non‐ionic detergent Tween 80 potentiated the cytotoxic action of colchicine on mutant cells as well as its rate of uptake into whole cells.
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Cited by
Expression of a multidrug-resistance gene in human tumors and tissues.
Fojo, A. T., Ueda, K., Slamon, D. J., Poplack, D. G., Gottesman, M. M., & Pastan, I. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.1.265 · Cited by 1,544
Cell Surface P-Glycoprotein Associated with Multidrug Resistance in Mammalian Cell Lines
Kartner, N., Riordan, J. R., & Ling, V. (1983) · Science · doi:10.1126/science.6137059 · Cited by 991
Self-citationLacey, E. (1988) · International Journal for Parasitology · doi:10.1016/0020-7519(88)90175-0 · Cited by 560
The molecular basis of multidrug resistance in cancer: The early years of P‐glycoprotein research
Gottesman, M. M., & Ling, V. (2006) · FEBS Letters · doi:10.1016/j.febslet.2005.12.060 · Cited by 558
Self-citationAutofluorescence of viable cultured mammalian cells.
Aubin, J. E. (1979) · Journal of Histochemistry & Cytochemistry · doi:10.1177/27.1.220325 · Cited by 555
Metadata
- Authors
- Ling, V., & Thompson, L. H.
- Venue
- Journal of Cellular Physiology
- Publisher
- Wiley
- Published
- 1974-02
- Volume
- 83
- Issue
- 1
- Pages
- 103-116
- ISSN
- 0021-9541 (ISSN-L)
- 1097-4652
- OpenAlex
- W2034031399
- Cited by
- 686
- References
- 0
- FWCI
- 8.70
- Type
- journal article
- Language
- en
- Open access
- closed
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