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Cited by 1Natural Antidiabetic Agents StudiesMedicinal Plant ResearchChemical synthesis and alkaloids

Validation of Blood Glucose and Lipid-Lowering Effect of Solvent Fractions of the Crinum Abyssinicum Shoot Tips in Streptozotocin-Induced Diabetic Mice

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Tegegne, Bantayehu Addis, Yihunie, Wubetu, Aschale, Yibeltal, Belew, Habtamu, Getachew, Melese·2024·Global Advances in Integrative Medicine and Health
DOI 10.1177/27536130231225464PMC PMC10788077
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Abstract

Background: Diabetes mellitus prevalence has reached epidemic levels despite the existence of contemporary treatments. People thus started looking at the possible therapeutic value of natural therapies. Crushed shoot tips of Crinum abyssinicum (Amaryllidaceae) are mixed with water in Ethiopia to treat diabetes, yet this practice is not well supported by science. Objective: In this experiment, mice models were used to verify the blood sugar and lipid-lowering benefits of solvent fractions of C. abyssinicum shoot tips. Materials and Methods: In a single-dose treated Streptozotocin (STZ)-induced diabetic model, mice were randomly grouped into eleven categories which include diabetic negative control, diabetic positive control, and 9 diabetic treatment groups. In repeated daily doses treated STZ-induced model, Mice were divided into 6 groups which included normal and diabetic negative control (TW80), diabetic positive control (5 mg/kg glibenclamide), and three diabetic treatment groups 100, 200, and 400 mg/kg). Finally, blood glucose, lipid level, and body weight were examined. Results: In the single-dose treated diabetic model, there was a significant blood glucose reduction at 200 and 400 mg/kg doses of aqueous fraction and glibenclamide starting from the sixth-hour post-administration unlike ethyl acetate and chloroform fraction compared to baseline and negative control. In repeated daily dose-treated diabetic mice, all three doses (100, 200, and 400 mg/kg of aqueous fraction) resulted in a substantial reduction ( P < .001) in blood glucose compared to baseline and negative control on the seventh day and 14 th day. Besides the AQF shows improvement in lipid levels and body weight parameters. Conclusion: The results of the study demonstrated that C. abyssinicum shoot tip fractions have the greatest potential to lower blood sugar and lipid levels, supporting conventional claims for the treatment of diabetes.

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  • Phytomedicine is a cost-effective alternative to synthetic medications for treating DM, offering fewer side effects, longer-lasting benefits, and improved patient compliance.

    “Phytomedicine is a cost-effective alternative to synthetic medications for treating DM, offering fewer side effects, longer-lasting benefits, and improved patient compliance.”

    From A critical review on diabetes mellitus type 1 and type 2 management approaches: from lifestyle modification to current and novel targets and therapeutic agents. (2024) · cites this paper as [130]

    The Introduction (page not available in our copy) describes herbal therapy as a treatment for diabetes and says medicinal herbs can have little toxicity or side effects. It does not establish that phytomedicine is more cost-effective than synthetic medications, provides longer-lasting benefits, or improves patient compliance. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

  • Herbals/botanicals have demonstrated potent anti-diabetic effects via a variety of mechanisms, such as enhancing insulin sensitivity, stimulating insulin secretion, blocking carbohydrate absorption, and reducing oxidative stress.

    “Herbals/botanicals have demonstrated potent anti-diabetic effects via a variety of mechanisms, such as enhancing insulin sensitivity, stimulating insulin secretion, blocking the absorption of carbohydrates, and reducing OS; the inhibition of α-glucosidase enzyme and the generation of advanced glycation products, the transcription of GLUT-4 and PPAR, and the presence of antioxidants.”

    From A critical review on diabetes mellitus type 1 and type 2 management approaches: from lifestyle modification to current and novel targets and therapeutic agents. (2024) · cites this paper as [130]

    The Introduction and Discussion (page not provided) describe increased insulin secretion, reduced intestinal glucose absorption, and antioxidant protection against oxidative stress as antidiabetic actions of medicinal plants. The Discussion also mentions increased glucose uptake by muscle and adipose tissue, but does not establish enhanced insulin sensitivity. The additional mechanisms in the printed sentence concerning advanced glycation products and GLUT-4 and PPAR transcription are not established in these passages. (LLM, conf=0.86)

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Research works printed but not linked: 33

  1. [1]Alam U Asghar O Azmi S Malik RA. Chapter 15 - general aspects of diabetes mellitus. In: Zochodne DW Malik RA, eds. Handbook of Clinical Neurology. Elsevier; 2014: 211- 222. 25410224
  2. [2]Alam S Hasan MK Neaz S Hussain N Hossain MF Rahman T. Diabetes mellitus: insights from epidemiology, biochemistry, risk factors, diagnosis, complications and comprehensive management. Diabetology (Basel). 2021; 2(2): 36- 50.
  3. [3]Abbas G Al Harrasi A Hussain H Hamaed A Supuran CT. The management of diabetes mellitus-imperative role of natural products against dipeptidyl peptidase-4, α-glucosidase and sodium-dependent glucose co-transporter 2 (SGLT2). Bioorg Chem. 2019; 86: 305- 315. 30738330
  4. [4]Asmamaw D Achamyeleh H. Assessment of medicinal plants and their conservation status in case of daligaw kebela, gozamen werda, East Gojjam Zone. J Biodivers Biopros Dev. 2018; 05(1): 170.
  5. [5]Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 2014; 4: 177. 24454289 PMC3887317
  6. [6]Aslan M Orhan N Orhan DD Ergun F. Hypoglycemic activity and antioxidant potential of some medicinal plants traditionally used in Turkey for diabetes. J Ethnopharmacol. 2010; 128(2): 384- 389. 20100559
  7. [7]Kooti W Farokhipour M Asadzadeh Z Ashtary-Larky D Asadi-Samani M. The role of medicinal plants in the treatment of diabetes: a systematic review. Electron Physician. 2016; 8(1): 1832- 1842. 26955456 PMC4768936
  8. [8]Regassa R. Assessment of indigenous knowledge of medicinal plant practice and mode of service delivery in Hawassa city, southern Ethiopia. J Med Plants Res. 2013; 7(9): 517- 535.
  9. [9]Meresa A Gemechu W Basha H Fekadu N Teka F Ashebir R, et al. Herbal medicines for the management of diabetic mellitus in Ethiopia and Eretria including their phytochemical constituents. AJADD. 2017; 05(01): 40- 58.
  10. [10]Berihun A Fekadu N Geleta B. An ethno botanical review on medicinal plants used for the management of hypertension. Clin Exp Pharmacol. 2017; 7(2).
  11. [12]Tegegne BA Mekuria AB Birru EM. Evaluation of anti-diabetic and anti-hyperlipidemic activities of hydro-alcoholic crude extract of the shoot tips of Crinum abyssinicum hochst. Ex A. Rich (amaryllidaceae) in mice. J Exp Pharmacol. 2022; 14: 27- 41. 35136357 PMC8817951
  12. [13]Zhang Q-W Lin L-G Ye W-C. Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med. 2018; 13(1): 20. 29692864 PMC5905184
  13. [14]Ayaz M Junaid M Ahmed J Ullah F Sadiq A Ahmad S, et al. Phenolic contents, antioxidant and anticholinesterase potentials of crude extract, subsequent fractions and crude saponins from Polygonum hydropiper L. BMC Complement Altern Med. 2014; 14(1): 145. 24884823 PMC4018186
  14. [15]Robinson S Sparrow S Williams B Decelle T Bertelsen T Reid K, et al. The European federation of the pharmaceutical industry and associations’ research and animal Welfare group: assessing and benchmarking ‘culture of care’in the context of using animals for scientific purpose. Lab Anim. 2020; 54(5): 421- 432. PMC7564339 31744380
  15. [16]Janet C Garber RWB Bielitzki JT Clayton LA. Guide for the care and use of laboratory animals. Eighth Edition. Washington, DC: Institute of Laboratory Animal Resources, National Research Council; 2010: 248.
  16. [18]Alene M Abdelwuhab M Belay A Yazie TS. Evaluation of antidiabetic activity of ajuga integrifolia (lamiaceae) root extract and solvent fractions in mice. Evid Based Complement Alternat Med. 2020; 2020: 6642588. 33424989 PMC7772015
  17. [19]Baquer NZ Kumar P Taha A Kale R Cowsik S McLean P. Metabolic and molecular action of Trigonella foenum-graecum (fenugreek) and trace metals in experimental diabetic tissues. J Biosci. 2011; 36(2): 383- 396. 21654091
  18. [20]Tamiru W Engidawork E Asres K. Evaluation of the effects of 80% methanolic leaf extract of Caylusea abyssinica (fresen.) fisch. and Mey. on glucose handling in normal, glucose loaded and diabetic rodents. BMC Complement Altern Med. 2012; 12(1): 151. 22967092 PMC3495218
  19. [21]King AJ. The use of animal models in diabetes research. Br J Pharmacol. 2012; 166(3): 877- 894. 22352879 PMC3417415
  20. [22]Hammeso WW Emiru YK Ayalew Getahun K Kahaliw W. Antidiabetic and antihyperlipidemic activities of the leaf latex extract of aloe megalacantha baker (aloaceae) in streptozotocin-induced diabetic model. Evid Based Complement Alternat Med. 2019; 2019: 8263786. 31178917 PMC6507245
  21. [23]Toma A Makonnen E Mekonnen Y Debella A Adisakwattana S. Antidiabetic activities of aqueous ethanol and n-butanol fraction of Moringa stenopetala leaves in streptozotocin-induced diabetic rats. BMC Complement Altern Med. 2015; 15(1): 242. 26187590 PMC4506633
  22. [24]Kamboj A Kumar S Kumar V. Evaluation of antidiabetic activity of hydroalcoholic extract of cestrum nocturnum leaves in streptozotocin-induced diabetic rats. Adv Pharmacol Sci. 2013; 2013: 150401. 24151502 PMC3787616
  23. [25]Gebremeskel L Beshir Tuem K Teklu T. Evaluation of antidiabetic effect of ethanolic leaves extract of becium grandiflorum lam.(lamiaceae) in streptozotocin-induced diabetic mice. Diabetes Metab Syndr Obes. 2020; 13: 1481- 1489. 32440177 PMC7210021
  24. [27]Noman OM Mothana RA Al-Rehaily AJ Al qahtani AS Nasr FA Khaled JM, et al. Phytochemical analysis and anti-diabetic, anti-inflammatory and antioxidant activities of Loranthus acaciae Zucc. Grown in Saudi Arabia. Saudi Pharm J. 2019; 27(5): 724- 730. 31297028 PMC6598209
  25. [28]Kumar R Gupta A Singh A Bishayee A Pandey AK. The antioxidant and antihyperglycemic activities of bottlebrush plant (callistemon lanceolatus) stem extracts. Stem Extracts. 2020; 7: 11. PMC7151608 32143382
  26. [29]Okokon JE Nyong ME. Antidiabetic and hypolipidemic activities of Zea mays husk extract and fractions. Journal of Herbs, Spices and Medicinal Plants. 2018; 24(2): 134- 150.
  27. [30]Nardos A Makonnen E Debella A. Effects of crude extracts and fractions of Moringa stenopetala (Baker f) Cufodontis leaves in normoglycemic and alloxan-induced diabetic mice. African Journal of Pharmacy and Pharmacology. 2010; 5.
  28. [31]Kifle ZD Anteneh DA Atnafie SA. Hypoglycemic, anti-hyperglycemic and anti-hyperlipidemic effects of bersama abyssinica fresen (melianthaceae) leaves' solvent fractions in normoglycemic and streptozotocin-induced diabetic mice. J Exp Pharmacol. 2020; 12: 385- 396. 33116953 PMC7568922
  29. [32]Tomai F Crea F Gaspardone A Versaci F De Paulis R Penta de Peppo A, et al. Ischemic preconditioning during coronary angioplasty is prevented by glibenclamide, a selective ATP-sensitive K+ channel blocker. Circulation. 1994; 90(2): 700- 705. 8044938
  30. [33]Nugent DA Smith DM Jones HB. A review of islet of Langerhans degeneration in rodent models of type 2 diabetes. Toxicol Pathol. 2008; 36(4): 529- 551. 18467681
  31. [34]Jayaprasad B Sharavanan P Sivaraj R. Antidiabetic effect of Chloroxylon swietenia bark extracts on streptozotocin induced diabetic rats. Beni-Suef University Journal of Basic and Applied Sciences. 2016; 5(1): 61- 69.
  32. [35]Daryabor G Atashzar MR Kabelitz D Meri S Kalantar K. The effects of type 2 diabetes mellitus on organ metabolism and the immune system. 2020; 11: 1582. PMC7387426 32793223
  33. [36]Ormazabal V Nair S Elfeky O Aguayo C Salomon C Zuñiga FA. Association between insulin resistance and the development of cardiovascular disease. Cardiovasc Diabetol. 2018; 17(1): 122. 30170598 PMC6119242

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Authors
Tegegne, B. A., Yihunie, W., Aschale, Y., Belew, H., & Getachew, M.
Affiliations
  • Debre Markos University (ET)
  • Department of Pharmacy, Debre Markos University, Debre Markos, Ethiopia
Corresponding author
Tegegne, Bantayehu Addis
ORCID
Venue
Global Advances in Integrative Medicine and Health
Publisher
SAGE Publications
Published
2024-01
Volume
13
Pages
27536130231225464-27536130231225464
ISSN
2753-6130 (ISSN-L)
DOI
10.1177/27536130231225464
PMC
PMC10788077
OpenAlex
W4390842959
Cited by
1
References
1
Citations by year
2024: 1
FWCI
0.33
Type
journal article
Language
en
Open access
Licence
Locations
  • Global Advances in Integrative Medicine and Health (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
University of Gondar
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
  • Physical Sciences › Chemistry › Organic Chemistry › Chemical synthesis and alkaloids
Keywords
diabetes mellitus, streptozotocin-induced diabetes, blood glucose reduction, hypolipidemic effect, solvent fractions, aqueous fraction, ethyl acetate fraction, chloroform fraction, glibenclamide, body weight, medicinal plants, traditional medicine

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