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An electron microscopic histochemical and analytical X-ray microprobe study of calcification in Bruch’s membrane from human eyes
Open accessAbstract
Transmission electron microscopy, electron microprobe analysis, high temperature microincineration, and electron microscopic histochemical procedures were used to study the electron-dense deposits characteristic of the macular aspect of aged human eyes. These inorganic deposits were rich in calcium and phosphorus and selectively removed by flotation on formic acid. The amorphous decalcified masses showed a significant sulfur peak and were readily stained with acidic phosphotungstic acid. The latter observations are indicative of the presence of organic matrical proteoglycan. Such data may be a further indication that proteoglycans are retained at sites of calcification.
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“Calcification with calcium phosphate deposition is also detected in the CEL with ageing [58], causing BrM to become stiffer.”
From Bruch’s Membrane: A Key Consideration with Complement-Based Therapies for Age-Related Macular Degeneration (2023) · cites this paper as [58]
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Metadata
- Authors
- Davis, W. L., Jones, R. G., & Hagler, H. K.
- Affiliations
- The University of Texas Southwestern Medical Center (US)
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1981-05
- Volume
- 29
- Issue
- 5
- Pages
- 601-608
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2145524477
- Cited by
- 34
- References
- 19
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 1 · 2018: 1 · 2017: 1 · 2015: 2 · 2014: 1 · 2013: 2 · 2012: 3
- FWCI
- 0.75
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- journals.sagepub.com/page/policies/text-and-data-mining-license (tdm)
- Locations
- Journal of Histochemistry & Cytochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Dermatological and Skeletal Disorders
- Health Sciences › Medicine › Ophthalmology › Retinal Development and Disorders
- Keywords
- Bruch's membrane, calcification, macular degeneration, age-related macular degeneration, calcium deposits, phosphorus, proteoglycans, sulfur, phosphotungstic acid staining, human eye
- MeSH terms
- Calcinosis, Electron Probe Microanalysis, Eye, Eye Diseases, Humans, Microscopy, Electron
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
