The DOI matches its Crossref record.
Mithramycin- and 4'-6-diamidino-2-phenylindole (DAPI)-DNA staining for fluorescence microspectrophotometric measurement of DNA in nuclei, plastids, and virus particles.
Abstract
The properties of two DNA-specific fluorochromes, 4'-6-diamidino-2-phenylindole (DAPI) and mithramycin, have been analyzed as reagents to quantitate cellular DNA by fluorescence microspectrophotometry. Optimal staining conditions and concentrations, and the effects of other cellular materials to which the dyes bind, have been evaluated in measurements of the DNA of rat, chick, and yeast nuclei, Gonyostomum chloroplasts, and T4 particles. Use of either fluorochrome permits a high degree of resolution of different DNA quantities in nuclei and in cell organelles, and the DAPI-DNA complex is sufficiently fluorescent to permit quantitation of the DNA content in genomes as small as those of individual T4 bacteriophage particles. Fluorescence of mithramycin- or DAPI-stained DNA is proportional to DNA quantity when DNA of the same has composition is compared. Quantitation does not appear to be affected discernably by the state of the DNA, whether in different stages of the cell cycle, in condensed chromosomes, or in noncycling, differentiated nuclei. The use of chicken red blood cells is recommended as an internal monitor for variations in staining conditions.
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Cited by
Corriveau, J. L., & Coleman, A. W. (1988) · American Journal of Botany · doi:10.1002/j.1537-2197.1988.tb11219.x · Cited by 848
Self-citationAndrés, V, & Walsh, K. (1996) · The Journal of cell biology · doi:10.1083/jcb.132.4.657 · Cited by 621
Infection of phytoplankton by viruses and reduction of primary productivity
Suttle, C. A., Chan, A. M., & Cottrell, M. T. (1990) · Nature · doi:10.1038/347467a0 · Cited by 543
BENTLEYLAWRENCE, J. (1988) · Cell · doi:10.1016/0092-8674(88)90530-2 · Cited by 532
Morales-Navarrete, H., Segovia-Miranda, F., Klukowski, P., Meyer, K., Nonaka, H., Marsico, G., Chernykh, M., Kalaidzidis, A., Zerial, M., & Kalaidzidis, Y. (2015) · eLife · doi:10.7554/elife.11214 · Cited by 98
Metadata
- Authors
- Coleman, A. W., Maguire, M. J., & Coleman, J. R.
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1981-08
- Volume
- 29
- Issue
- 8
- Pages
- 959-968
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W1976017022
- Cited by
- 238
- References
- 0
- FWCI
- 10.08
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Mithramycin- and 4'-6-diamidino-2-phenylindole (DAPI)-DNA staining for fluorescence microspectrophotometric measurement of DNA in nuclei, plastids, and virus particles. https://app.cochat.ai/papers/10.1177/29.8.6168681
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- Coleman, A. W., Maguire, M. J., & Coleman, J. R. (1981). Mithramycin- and 4'-6-diamidino-2-phenylindole (DAPI)-DNA staining for fluorescence microspectrophotometric measurement of DNA in nuclei, plastids, and virus particles. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1177/29.8.6168681
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