The DOI matches its Crossref record.
Combined effects of Notch signaling and cytokines induce a multiple log increase in precursors with lymphoid and myeloid reconstituting ability
Open accessAbstract
We investigated whether combined signaling induced by engineered Notch ligands and hematopoietic growth factors influences hematopoietic stem-cell differentiation. We show that incubation of murine marrow precursors with Delta1ext-IgG, a Notch ligand consisting of the Delta1 extracellular domain fused to the Fc portion of human immunoglobulin G1 (IgG1), and growth factors stem cell factor (SCF), interleukin 6 (IL-6), IL-11, and Flt3-l inhibited myeloid differentiation and promoted a several-log increase in the number of precursors capable of short-term lymphoid and myeloid repopulation. Addition of IL7 promoted early T-cell development, whereas addition of granulocyte-macrophage colony-stimulating factor (GM-CSF) led to terminal myeloid differentiation. These results support a role for combinatorial effects by Notch and cytokine-induced signaling pathways in regulating hematopoietic cell fate and suggest the usefulness of Notch ligand in increasing hematopoietic precursor numbers for clinical stem-cell transplantation.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Delaney, C., Heimfeld, S., Brashem-Stein, C., Voorhies, H., Manger, R. L., & Bernstein, I. D. (2010) · Nature Medicine · doi:10.1038/nm.2080 · Cited by 771
Self-citationNotch Signaling in the Immune System
Radtke, F., Fasnacht, N., & MacDonald, H. R. (2010) · Immunity · doi:10.1016/j.immuni.2010.01.004 · Cited by 543
Notch regulation of lymphocyte development and function
Radtke, F., Wilson, A., Mancini, S. J. C., & MacDonald, H. R. (2004) · Nature Immunology · doi:10.1038/ni1045 · Cited by 539
Hematopoietic stem cell fate is established by the Notch-Runx pathway
Burns, C. E., Traver, D., Mayhall, E., Shepard, J. L., & Zon, L. I. (2005) · Genes & Development · doi:10.1101/gad.1337005 · Cited by 401
Signaling pathways governing stem-cell fate
Blank, U., Karlsson, G., & Karlsson, S. (2008) · Blood · doi:10.1182/blood-2007-07-075168 · Cited by 355
Metadata
- Authors
- Varnum-Finney, B., Brashem-Stein, C., & Bernstein, I. D.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2003-03-01
- Volume
- 101
- Issue
- 5
- Pages
- 1784-1789
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2051292725
- Cited by
- 267
- References
- 0
- FWCI
- 10.09
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Combined effects of Notch signaling and cytokines induce a multiple log increase in precursors with lymphoid and myeloid reconstituting ability. https://app.cochat.ai/papers/10.1182/blood-2002-06-1862
- The paper
- Varnum-Finney, B., Brashem-Stein, C., & Bernstein, I. D. (2003). Combined effects of Notch signaling and cytokines induce a multiple log increase in precursors with lymphoid and myeloid reconstituting ability. Blood. https://doi.org/10.1182/blood-2002-06-1862
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
