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Arginase I is constitutively expressed in human granulocytes and participates in fungicidal activity
Open accessAbstract
The balance of arginine metabolism via nitric oxide synthase (NOS) or arginase is an important determinant of the inflammatory response of murine macrophages and dendritic cells. Here we analyzed the expression of the isoform arginase I in human myeloid cells. Using healthy donors and patients with arginase I deficiency, we found that in human leukocytes arginase I is constitutively expressed only in granulocytes and is not modulated by a variety of proinflammatory and anti-inflammatory stimuli in vitro. We demonstrate that arginase I is localized in azurophil granules of neutrophils and constitutes a novel antimicrobial effector pathway, likely through arginine depletion in the phagolysosome. Our findings demonstrate important differences between murine and human leukocytes with respect to regulation and function of arginine metabolism via arginase.
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“Importantly, in contrast to rodent Mp, human M2a Mp or monocytes do not express arginase [36,37].”
From Macrophage phenotypes during tissue repair (2013) · cites this paper as [36]
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Metadata
- Authors
- Munder, M.
- Affiliations
- University Hospital Heidelberg (DE)
- Corresponding author
- Munder, M.
- ORCID
- Munder, M.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2005-03-15
- Volume
- 105
- Issue
- 6
- Pages
- 2549-2556
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2108273635
- Cited by
- 335
- References
- 42
- Citations by year
- 2026: 7 · 2025: 12 · 2024: 12 · 2023: 13 · 2022: 14 · 2021: 19 · 2020: 15 · 2019: 18 · 2018: 12 · 2017: 15 · 2016: 17 · 2015: 20 · 2014: 28 · 2013: 25 · 2012: 17
- FWCI
- 3.33
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Digital Library (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Keywords
- arginase-1, granulocytes, neutrophils, arginine metabolism, nitric oxide synthase, fungicidal activity, azurophilic granules, arginine deprivation, phagolysosomes, myeloid cells, inflammation
- MeSH terms
- Animals, Antifungal Agents, Arginase, Arginine, Humans, Isoenzymes, Macrophages, Neutrophils, Phagosomes, Species Specificity, Gene Expression Regulation, Enzymologic, Nitric Oxide Synthase, Hyperargininemia, Secretory Vesicles, Microscopy, Electron, Transmission, Mice
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