The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
The earliest subpopulation of mouse thymocytes contains potent T, significant macrophage, and natural killer cell but no B-lymphocyte potential
Abstract
The B-lymphocyte potential of progenitor thymocytes and whether the thymus is colonized by common lymphocyte progenitor cells have been subjects of considerable debate. Herein, we have used limiting dilution analysis to determine the lineage potential of phenotypically defined subpopulations of CD4–CD8– double-negative thymocytes. Culture systems used showed single-hit kinetics and had a high plating efficiency for B-, myeloid, and natural killer cell development. The T-cell potential of sorted cells was confirmed by transferring cells to fetal thymus organ cultures. Our results indicate that the earliest population of CD117+ double-negative cells, although containing potent T-cell developmental potential and significant myeloid and natural killer potential, does not have any residual B-cell potential. Gene transcription analysis also indicated that these double-negative cells contained abundant T and myeloid, but not B cell–specific transcripts. The implications of these results within the context of current models of thymocyte development are discussed.
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“Whereas ETP and DN2 cells can generate myeloid cells if removed from the thymus, pro-T cells that have differentiated past the stage of PU.1 expression in the thymus (i.e., from the DN2 stage to the DN3 stage or later) do not make myeloid cells under these conditions”
From Mechanisms of Action of Hematopoietic Transcription Factor PU.1 in Initiation of T-Cell Development (2019) · cites this paper as [54]
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Adult T-cell progenitors retain myeloid potential
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Notch signaling controls the generation and differentiation of early T lineage progenitors
Sambandam, A., Maillard, I. P., Zediak, V. P., Xu, L., Gerstein, R. M., Aster, J. C., Pear, W. S., & Bhandoola, A. (2005) · Nature Immunology · doi:10.1038/ni1216 · Cited by 364
Metadata
- Authors
- Balciunaite, G., Ceredig, R., & Rolink, A. G.
- Affiliations
- From the Developmental and Molecular Immunology, Department of Clinical and Biological Sciences (DKBW), University of Basel, Basel, Switzerland; and U548 INSERM, DRDC, CEA-G, Grenoble, France.
- Inserm (FR)
- ORCID
- Ceredig, Rhodri
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2005-03-01
- Volume
- 105
- Issue
- 5
- Pages
- 1930-1936
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2062319579
- Cited by
- 158
- References
- 55
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 3 · 2021: 7 · 2020: 3 · 2019: 9 · 2018: 6 · 2017: 2 · 2016: 6 · 2015: 4 · 2014: 5 · 2013: 6 · 2012: 3
- FWCI
- 5.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- T cell development, double-negative thymocytes, CD4+CD8+ thymocytes, macrophage development, natural killer cell development, CD117, fetal thymus organ culture, gene transcription, single-hit kinetics
- MeSH terms
- Animals, B-Lymphocytes, Cells, Cultured, Hematopoietic Stem Cells, Immune System, Killer Cells, Natural, Kinetics, Macrophages, T-Lymphocytes, Thymus Gland, Immunophenotyping, Cell Lineage, Leukopoiesis, Mice
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