Cited by 158T-cell and B-cell ImmunologyHematopoietic Stem Cell TransplantationDevelopmental Biology and Gene Regulation

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The earliest subpopulation of mouse thymocytes contains potent T, significant macrophage, and natural killer cell but no B-lymphocyte potential

Balciunaite, Gina, Ceredig, Rhodri, Rolink, Antonius G.·2005·Blood

DOI 10.1182/blood-2004-08-3087

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Abstract

The B-lymphocyte potential of progenitor thymocytes and whether the thymus is colonized by common lymphocyte progenitor cells have been subjects of considerable debate. Herein, we have used limiting dilution analysis to determine the lineage potential of phenotypically defined subpopulations of CD4–CD8– double-negative thymocytes. Culture systems used showed single-hit kinetics and had a high plating efficiency for B-, myeloid, and natural killer cell development. The T-cell potential of sorted cells was confirmed by transferring cells to fetal thymus organ cultures. Our results indicate that the earliest population of CD117+ double-negative cells, although containing potent T-cell developmental potential and significant myeloid and natural killer potential, does not have any residual B-cell potential. Gene transcription analysis also indicated that these double-negative cells contained abundant T and myeloid, but not B cell–specific transcripts. The implications of these results within the context of current models of thymocyte development are discussed.

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Authors: Balciunaite, G., Ceredig, R., & Rolink, A. G.
Affiliations: From the Developmental and Molecular Immunology, Department of Clinical and Biological Sciences (DKBW), University of Basel, Basel, Switzerland; and U548 INSERM, DRDC, CEA-G, Grenoble, France.
Inserm (FR)
ORCID: Ceredig, Rhodri
Venue: Blood
Publisher: American Society of Hematology
Published: 2005-03-01
Volume: 105
Issue: 5
Pages: 1930-1936
ISSN: 0006-4971 (ISSN-L)
1528-0020
DOI: 10.1182/blood-2004-08-3087
OpenAlex: W2062319579
Cited by: 158
References: 55
Citations by year: 2026: 2 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 3 · 2021: 7 · 2020: 3 · 2019: 9 · 2018: 6 · 2017: 2 · 2016: 6 · 2015: 4 · 2014: 5 · 2013: 6 · 2012: 3
FWCI: 5.77
Type: journal article
Language: en
Open access: closed
Locations: Blood (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords: T cell development, double-negative thymocytes, CD4+CD8+ thymocytes, macrophage development, natural killer cell development, CD117, fetal thymus organ culture, gene transcription, single-hit kinetics
MeSH terms: Animals, B-Lymphocytes, Cells, Cultured, Hematopoietic Stem Cells, Immune System, Killer Cells, Natural, Kinetics, Macrophages, T-Lymphocytes, Thymus Gland, Immunophenotyping, Cell Lineage, Leukopoiesis, Mice

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The earliest subpopulation of mouse thymocytes contains potent T, significant macrophage, and natural killer cell but no B-lymphocyte potential

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