The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak–like cells and enables isolation of primitive hematopoietic precursors
Open accessAbstract
Differentiating human embryonic stem cells (HESCs) represent an experimental platform for establishing the relationships between the earliest lineages that emerge during human development. Here we report the targeted insertion in HESCs of sequences encoding green fluorescent protein (GFP) into the locus of MIXL1, a gene transiently expressed in the primitive streak during embryogenesis.1,2 GFP fluorescence in MIXL1GFP/w HESCs differentiated in the presence of BMP4 reported the expression of MIXL1, permitting the identification of viable human primitive streak-like cells. The use of GFP as a reporter for MIXL1 combined with cell surface staining for platelet-derived growth factor receptor alpha (PDGFRα) enabled the isolation of a cell population that was highly enriched in primitive hematopoietic precursors, the earliest derivatives of the primitive streak. These experiments demonstrate the utility of MIXL1GFP/w HESCs for analyzing the previously inaccessible events surrounding the development of human primitive streak-like cells and their subsequent commitment to hematopoiesis.
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Cites
Mixl1 is required for axial mesendoderm morphogenesis and patterning in the murine embryo
Hart, A. H., Hartley, L., Sourris, K., Stadler, E. S., Li, R., Stanley, E. G., Tam, P. P. L., Elefanty, A. G., & Robb, L. (2002) · Development · doi:10.1242/dev.129.15.3597 · Cited by 229
Self-citationTam, P. P. L., Khoo, P.-L., Lewis, S. L., Bildsoe, H., Wong, N., Tsang, T. E., Gad, J. M., & Robb, L. (2007) · Development · doi:10.1242/dev.02724 · Cited by 94
Cited by
Lian, X., Hsiao, C., Wilson, G., Zhu, K., Hazeltine, L. B., Azarin, S. M., Raval, K. K., Zhang, J., Kamp, T. J., & Palecek, S. P. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1200250109 · Cited by 1,717
Induced Pluripotent Stem Cells Meet Genome Editing
Hockemeyer, D., & Jaenisch, R. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2016.04.013 · Cited by 499
“Protocols for conventional gene targeting have been optimized to modify human pluripotent stem cells (Costa et al., 2007; Davis et al., 2008a; Irion et al., 2007; Ruby and Zheng, 2009) and have been successfully used to establish hPSC models for human disease such as Lesch-Nyhan syndrome (Urbach et…”
MethodSturgeon, C. M., Ditadi, A., Awong, G., Kennedy, M., & Keller, G. (2014) · Nature Biotechnology · doi:10.1038/nbt.2915 · Cited by 467
Human heart-forming organoids recapitulate early heart and foregut development
Drakhlis, L., Biswanath, S., Farr, C.-M., Lupanow, V., Teske, J., Ritzenhoff, K., Franke, A., Manstein, F., Bolesani, E., Kempf, H., et al. (2021) · Nature Biotechnology · doi:10.1038/s41587-021-00815-9 · Cited by 452
NKX2-5eGFP/w hESCs for isolation of human cardiac progenitors and cardiomyocytes
Elliott, D. A., Braam, S. R., Koutsis, K., Ng, E. S., Jenny, R., Lagerqvist, E. L., Biben, C., Hatzistavrou, T., Hirst, C. E., Yu, Q. C., et al. (2011) · Nature Methods · doi:10.1038/nmeth.1740 · Cited by 426
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Metadata
- Authors
- Davis, R. P., Ng, E. S., Costa, M., Mossman, A. K., Sourris, K., Elefanty, A. G., & Stanley, E. G.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2008-02-15
- Volume
- 111
- Issue
- 4
- Pages
- 1876-1884
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2088101102
- Cited by
- 235
- References
- 2
- FWCI
- 6.82
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak–like cells and enables isolation of primitive hematopoietic precursors. https://app.cochat.ai/papers/10.1182/blood-2007-06-093609
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- Davis, R. P., Ng, E. S., Costa, M., Mossman, A. K., Sourris, K., Elefanty, A. G., & Stanley, E. G. (2008). Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak–like cells and enables isolation of primitive hematopoietic precursors. Blood. https://doi.org/10.1182/blood-2007-06-093609
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