Cited by 229Developmental Biology and Gene RegulationCongenital heart defects researchPluripotent Stem Cells Research

Mixl1 is required for axial mesendoderm morphogenesis and patterning in the murine embryo

Hart, Adam H., Hartley, Lynne, Sourris, Koula, Stadler, Elizabeth S., Li, Ruili, Stanley, Edouard G., Tam, Patrick P. L., Elefanty, Andrew G. +1 more·2002·Development

Abstract

In Xenopus, the Mix/Bix family of homeobox genes has been implicated in mesendoderm development. Mixl1 is the only known murine member of this family. To examine the role of Mixl1 in murine embryogenesis, we used gene targeting to create mice bearing a null mutation of Mixl1. Homozygous Mixl1 mutant embryos can be distinguished from their littermates by a marked thickening of the primitive streak. By the early somite stage, embryonic development is arrested, with the formation of abnormal head folds, foreshortened body axis, absence of heart tube and gut, deficient paraxial mesoderm, and an enlarged midline tissue mass that replaces the notochord. Development of extra-embryonic structures is generally normal except that the allantois is often disproportionately large for the size of the mutant embryo. In chimeras, Mixl1–/– mutant cells can contribute to all embryonic structures, with the exception of the hindgut, suggesting that Mixl1 activity is most crucial for endodermal differentiation. Mixl1 is therefore required for the morphogenesis of axial mesoderm, the heart and the gut during embryogenesis.

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Crossref · 10.1242/dev.129.15.3597

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Authors
Hart, A. H., Hartley, L., Sourris, K., Stadler, E. S., Li, R., Stanley, E. G., Tam, P. P. L., Elefanty, A. G., & Robb, L.
Affiliations
  • The Royal Melbourne Hospital (AU)
  • Children's Medical Research Institute (AU)
Venue
Development
Published
2002-08-01
Volume
129
Issue
15
Pages
3597-3608
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
OpenAlex
W2188419093
Cited by
229
References
68
Citations by year
2026: 2 · 2025: 9 · 2024: 6 · 2023: 10 · 2022: 6 · 2021: 5 · 2020: 8 · 2019: 7 · 2018: 4 · 2017: 7 · 2016: 10 · 2015: 13 · 2014: 11 · 2013: 9 · 2012: 12
FWCI
2.89
Type
journal article
Language
en
Open access
closed
Locations
  • Development (publishedVersion)
  • PubMed (publishedVersion)
  • HighWire Press Open Archive (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords
Mixl1, primitive streak, heart development, intestinal development, endoderm differentiation, paraxial mesoderm, notochord, allantois, embryonic lethality, chimeras, mouse embryo
MeSH terms
Brachyury Protein, Animals, Embryo, Mammalian, Embryo, Nonmammalian, Endoderm, Embryonic and Fetal Development, Fetal Proteins, Mesoderm, Morphogenesis, Stem Cells, Zebrafish, Signal Transduction, Transforming Growth Factor beta, In Situ Hybridization, Genes, Reporter, Mice, Knockout, Gene Targeting, Homeodomain Proteins, Body Patterning, T-Box Domain Proteins, Mice, Nodal Protein

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