The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
NADPH oxidase controls phagosomal pH and antigen cross-presentation in human dendritic cells
Open accessAbstract
The phagocyte NADPH oxidase (NOX2) is critical for the bactericidal activity of phagocytic cells and plays a major role in innate immunity. We showed recently that NOX2 activity in mouse dendritic cells (DCs) prevents acidification of phagosomes, promoting antigen cross-presentation. Inorder to investigate the role of NOX2 in the regulation of the phagosomal pH in human DCs, we analyzed the production of reactive oxygen species (ROS) and the phagosomal/endosomal pH in monocyte-derived DCs and macrophages (MØs) from healthy donors or patients with chronic granulomatous disease (CGD). As expected, we found that human MØs acidify their phagosomes more efficiently than human DCs. Accordingly, the expression of the vacuolar proton ATPase (V-H+-ATPase) was higher in MØs than in DCs. Phagosomal ROS production, however, was also higher in MØs than in DCs, due to higher levels of gp91phox expression and recruitment to phagosomes. In contrast, in the absence of active NOX2, the phagosomal and endosomal pH decreased. Both in the presence of a NOX2 inhibitor and in DCs derived from patients with CGD, the cross-presentation of 2 model tumor antigens was impaired. We conclude that NOX2 activity participates in the regulation of the phagosomal and endosomal pH in human DCs, and is required for efficient antigen cross-presentation.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Dendritic cells (DC) express little NOX2 and consequently have a lower O2•− and ROS production after activation [179].”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [179]
Citations
Cites
Meijer, L., Nelson, D. J., Riazanski, V., Gabdoulkhakova, A. G., Héry-Arnaud, G., Berre, R. L., Loaëc, N., Oumata, N., Galons, H., Nowak, E., et al. (2016) · Journal of Innate Immunity · doi:10.1159/000444256 · Cited by 5,008
Apetoh, L., Ghiringhelli, F., Tesniere, A., Obeid, M., Ortiz, C., Criollo, A., Mignot, G., Maiuri, M. C., Ullrich, E., Saulnier, P., et al. (2007) · Nature Medicine · doi:10.1038/nm1622 · Cited by 3,056
Self-citationSavina, A., Jancic, C. C., Hugues, S., Guermonprez, P., Vargas, P., Moura, I. C., Lennon‐Duménil, A., Seabra, M. C., Raposo, G., & Amigorena, S. (2006) · Cell · doi:10.1016/j.cell.2006.05.035 · Cited by 896
Self-citationCross-Presentation, Dendritic Cells, Tolerance and Immunity
Heath, W. R., & Carbone, F. R. (2001) · Annual Review of Immunology · doi:10.1146/annurev.immunol.19.1.47 · Cited by 882
Differential Lysosomal Proteolysis in Antigen-Presenting Cells Determines Antigen Fate
Delamarre, L., Pack, M., Chang, H. C., Mellman, I., & Trombetta, E. S. (2005) · Science · doi:10.1126/science.1108003 · Cited by 776
ER–phagosome fusion defines an MHC class I cross-presentation compartment in dendritic cells
Guermonprez, P., Saveanu, L., Kleijmeer, M. J., Davoust, J. M., Endert, P. V., & Amigorena, S. (2003) · Nature · doi:10.1038/nature01911 · Cited by 753
Self-citationRodrı́guez, A., Régnault, A., Kleijmeer, M. J., Ricciardi‐Castagnoli, P., & Amigorena, S. (1999) · Nature Cell Biology · doi:10.1038/14058 · Cited by 552
Self-citationCFTR regulates phagosome acidification in macrophages and alters bactericidal activity
Di, A., Brown, M. E., Deriy, L. V., Li, C., Szeto, F. L., Chen, Y., Huang, P. C., Tong, J., Naren, A. P., Bindokas, V. P., et al. (2006) · Nature Cell Biology · doi:10.1038/ncb1456 · Cited by 516
The NADPH oxidase of professional phagocytes—prototype of the NOX electron transport chain systems
Cross, A. R., & Segal, A. W. (2004) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2004.03.008 · Cited by 489
Cross‐presentation: underlying mechanisms and role in immune surveillance
Rock, K. L., & Shen, L. (2005) · Immunological Reviews · doi:10.1111/j.0105-2896.2005.00301.x · Cited by 469
Cited by
Sorted by citations
Items 301–329 of 329
Reactive Oxygen Species, Central Regulators of the Tumor Microenvironment
Lamberti, M. J., Vera, R. E., Fernández-Zapico, M. E., & Vittar, N. B. R. (2021) · Springer eBooks · doi:10.1007/978-981-15-4501-6_159-1
Coria, L. M. (2014) · Repositorio Digital Institucional de la Universidad de Buenos Aires (Universidad de Buenos Aires)
Reactive Oxygen Species: Central Regulators of the Tumor Microenvironment
Lamberti, M. J., Vera, R. E., Fernández-Zapico, M. E., & Vittar, N. B. R. (2022) · Springer eBooks · doi:10.1007/978-981-15-9411-3_159
Morel, L. M. (2012) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.14130956.15622056
Mechanisms Of Organelle Identity and Adaptation In Immune Cells
Hipolito, V. E. B. (2021) · doi:10.32920/ryerson.14668524
Mechanisms Of Organelle Identity and Adaptation In Immune Cells
Hipolito, V. E. B. (2021) · doi:10.32920/ryerson.14668524.v1
Faculty Opinions recommendation of Activation of antibacterial autophagy by NADPH oxidases.
Neish, A. S. (2009) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.1160816.623275
Oxidative stress indicators during the course of acute graft versus host disease
Şahin, U., & Dursun, A. D. (2022) · Journal of Health Sciences and Medicine · doi:10.32322/jhsm.1172571
Lysosome remodelling and adaptation during phagocyte activation
Hipolito, V. E. B., Escobar, E. O., & Botelho, R. (2023) · doi:10.32920/21982895.v1
Rafei, M., Bikorimana, J., Farah, R., Abusarah, J., Erregragui, M. A., Gonçalves, M., Talbot, S., & El-Hachem, N. (2023) · Research Square · doi:10.21203/rs.3.rs-3545973/v1
Zheng, J., Wang, L., Zhao, S., Ayasoufi, K., Goddery, E. N., Strege, P. R., Wan, Y., Zhang, W., Xie, M., Beyder, A., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.10.18.619142
Bikorimana, J., Farah, R., Abusarah, J., Mandl, G. A., Erregragui, M. A., Pereira, M. A., Talbot, S., Matar, P., Lahrichi, M., El-Hachem, N., et al. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.5013530
Forced intracellular degradation of xenoantigens as a modality for cell-based cancer immunotherapy
Bikorimana, J., Farah, R., Abusarah, J., Mandl, G. A., Erregragui, M. A., Gonçalves, M. P., Talbot, S., Matar, P., Lahrichi, M., El-Hachem, N., et al. (2025) · iScience · doi:10.1016/j.isci.2025.111957
Laub, M., Chatterjee, R., & Kozik, P. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2025.12.03.692202
Essential role of MHC II in the antitubercular efficacy of pyrazinamide
Lamont, E. A., Kordus, S. L., Howe, M. D., Jia, Z., Schacht, N. A., Rather, M. A., Gebretsadik, G., & Baughn, A. D. (2025) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01264-25
Kourjian, G. (2015) · theses.fr (ABES) · doi:10.70675/b41647f7zdd56z465cz9213zc11fff98c2ca
Hui, L. I., Yu, D. I. N. G., & Xie, Z. (2026) · Scientia Sinica Vitae · doi:10.1360/ssv-2026-0054
Dendritic cells as central coordinators of oncolytic virus-induced antitumor immunity
Ezzaldeen, E. M., Vijayan, V. V., Nair, P. G., Unnikrishnan, B. S., Banerjee, A., & Gujar, S. A. (2026) · Seminars in Immunology · doi:10.1016/j.smim.2026.102033
Disulfidptosis-based nanomedicines for targeting tumor metabolic vulnerabilities
Cai, Y., Huang, T., Shi, X., & Yang, Y. (2026) · Coordination Chemistry Reviews · doi:10.1016/j.ccr.2026.218139
Cheng, Z., Wu, S., Li, S., Yu, X., Gao, Y., & Zheng, J. (2026) · Qeios · doi:10.32388/5i9sqw
Unveiling the role of lysosomes in antigen processing and presentation
Colangelo, G. S., Cowan, K. J., Sirtori, F. R., & Barbero, L. M. (2026) · The Journal of Immunology · doi:10.1093/jimmun/vkag200
NOX2 deficiency exacerbates lupus nephritis through activation of NLRP3 inflammasome in neutrophils
Wu, C., Lin, F., Lin, M.-S., Chan, T., Chen, Y.-R., Cheng, H.-C., Hsu, C.-K., Chen, C.-A., Chen, P., Cheng, S.-M., et al. (2026) · Journal of Biomedical Science · doi:10.1186/s12929-026-01287-2
Anchoring of perforin-2 via the transmembrane domain is required for antigen escape into the cytosol
Laub, M., Chatterjee, R., & Kozik, P. (2026) · The EMBO Journal · doi:10.1038/s44318-026-00903-z
Beyond cold to hot: oncolytic virotherapy as the next cornerstone of immuno-oncology
Jia, W. W.-G., Zhao, R., Kaufman, H. L., & Martuza, R. L. (2026) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-026-01198-z
Fan, L., Pang, Y., Wang, L., Kechiyerunda, D. C., Zhang, B., & Liang, J. (2026) · Redox Biology · doi:10.1016/j.redox.2026.104388
Ju, M., Zhang, M., Ma, S., An, Y., Xiao, S., Xu, J., Li, N., Chen, Y., Wei, M., Wang, H., et al. (2026) · Journal of Advanced Research · doi:10.1016/j.jare.2026.10.003
The Role of NCF4 in Adaptive Immunity and Autoimmune Diseases
王, 唯. (2026) · Medical Diagnosis · doi:10.12677/md.2026.165068
Modulation fonctionnelle des cellules dendritiques par les « Neutrophil Extracellular Traps »
Barrientos, L. (2013) · theses.fr (ABES) · doi:10.70675/d74489c5z4048z43e4za7ebz0dd46aa98d1b
Metadata
- Authors
- Mantegazza, A. R., Savina, A., Vermeulen, M., Pérez, L., Geffner, J., Hermine, O., Rosenzweig, S. D., Faure, F., & Amigorena, S.
- Affiliations
- Inserm (FR)
- Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, Argentina;
- Garrahan Hospital (AR)
- Academia Nacional de Medicina (AR)
- Hôpital Necker-Enfants Malades (FR)
- ORCID
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2008-12-01
- Volume
- 112
- Issue
- 12
- Pages
- 4712-4722
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- PMC
- PMC2597138
- OpenAlex
- W2093168274
- Cited by
- 329
- References
- 34
- Citations by year
- 2026: 14 · 2025: 16 · 2024: 17 · 2023: 26 · 2022: 23 · 2021: 22 · 2020: 28 · 2019: 17 · 2018: 22 · 2017: 18 · 2016: 15 · 2015: 18 · 2014: 8 · 2013: 15 · 2012: 28
- FWCI
- 5.84
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Blood (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- CONICET Digital (CONICET) (submittedVersion, cc-by-nc-sa)
- Funders
- Institut National de la Santé et de la Recherche Médicale
- Consejo Nacional de Investigaciones Científicas y Técnicas
- National Institutes of Health
- Directorate for Biological Sciences
- Fogarty International Center (R01TW006644)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- NADPH oxidase, NOX2, dendritic cells, phagosomal pH, cross-presentation, reactive oxygen species, gp91phox, vacuolar ATPase, chronic granulomatous disease, monocyte-derived macrophages, endosomal pH, innate immunity
- MeSH terms
- NADPH Oxidase 2, Acid-Base Equilibrium, Adolescent, Adult, Cells, Cultured, Child, Child, Preschool, Dendritic Cells, Humans, Hydrogen-Ion Concentration, Macrophages, Membrane Glycoproteins, Phagosomes, Antigen Presentation, NADPH Oxidases, Cross-Priming, Young Adult
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
