Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages

Lamar, David L.; Weyand, Cornelia M.; Goronzy, Jörg J.

doi:10.1182/blood-2009-09-243493

Cited by 45Immune Cell Function and InteractionT-cell and B-cell ImmunologyReproductive System and Pregnancy

Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages

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Lamar, David L., Weyand, Cornelia M., Goronzy, Jörg J.·2010·Blood

DOI 10.1182/blood-2009-09-243493PMC PMC2858479

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Abstract

CD85j (ILT2/LILRB1/LIR-1) is an inhibitory receptor that recognizes major histocompatibility complex (MHC) class Ia and Ib alleles that are widely expressed on all cell types. On ligand recognition, CD85j diminishes kinase activity by recruiting phosphatases to motifs within its cytoplasmic domain. Within the hematopoietic system, CD85j is expressed with cell-specific patterns and cell surface densities that reflect the different roles of cell contact-mediated inhibition in these lineages. While monocytes ubiquitously have high cell surface expression, B lymphocytes start to express CD85j at intermediate levels during early B-cell maturation and natural killer (NK) cells and T cells exhibit a low level of expression on only a subset of cells. The cell-specific expression pattern is accomplished by 2 complementing but not independent mechanisms. Lymphocytes and monocytes use distinct promoters to drive CD85j expression. The lymphocyte promoter maps 13 kilobases (kb) upstream of the monocyte promoter; its use results in the inclusion of a distant exon into the 5′-untranslated region. A short sequence stretch within this exon has the unique function of repressing CD85j protein translation and is responsible for the subdued expression in lymphocytes. These cell-specific mechanisms allow tailoring of CD85j levels to the distinct roles it plays in different hematopoietic lineages.

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TEMRAs express negative regulatory receptors such as killer immunoglobulin-like receptors and CD85j that interfere with their ability to proliferate but not with cytokine production.
“TEMRAs express a host of negative regulatory receptors such as killer immunoglobulin-like receptors and CD85j that interfere with their ability to proliferate but not with cytokine production”
From Successful and Maladaptive T Cell Aging (2017) · cites this paper as Lamar et al., 2010

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Mosaic Analysis with a Repressible Cell Marker for Studies of Gene Function in Neuronal Morphogenesis
Lee, T., & Luo, L. (1999) · Neuron · doi:10.1016/s0896-6273(00)80701-1 · Cited by 2,540 · see citing works
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences
Mammalian Gene Collection (MGC) Program Team* (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.242603899 · Cited by 1,742 · see citing works
NK CELL RECEPTORS
Lanier, L. L. (1998) · Annual Review of Immunology · doi:10.1146/annurev.immunol.16.1.359 · Cited by 1,641 · see citing works
B Cell Development Pathways
Hardy, R. R., & Hayakawa, K. (2001) · Annual Review of Immunology · doi:10.1146/annurev.immunol.19.1.595 · Cited by 1,236 · see citing works
Monocyte-Mediated Defense Against Microbial Pathogens
Serbina, N. V., Jia, T., Hohl, T. M., & Pamer, E. G. (2008) · Annual Review of Immunology · doi:10.1146/annurev.immunol.26.021607.090326 · Cited by 1,116 · see citing works
AU-rich elements and associated factors: are there unifying principles?
Barreau, C. (2005) · Nucleic Acids Research · doi:10.1093/nar/gki1012 · Cited by 1,033 · see citing works
A Common Inhibitory Receptor for Major Histocompatibility Complex Class I Molecules on Human Lymphoid and Myelomonocytic Cells
Colonna, M., Navarro, F., Bellón, T., Llano, M., Garcı́a, P., Samaridis, J., Ångman, L., Cella, M., & López‐Botet, M. (1997) · The Journal of Experimental Medicine · doi:10.1084/jem.186.11.1809 · Cited by 948 · see citing works
Functionally and Structurally Distinct NK Cell Receptor Repertoires in the Peripheral Blood of Two Human Donors
Valiante, N. M., Uhrberg, M., Shilling, H. G., Lienert-Weidenbach, K., Arnett, K. L., D’Andrea, A., Phillips, J. H., Lanier, L. L., & Parham, P. (1997) · Immunity · doi:10.1016/s1074-7613(00)80393-3 · Cited by 726 · see citing works
A Novel Immunoglobulin Superfamily Receptor for Cellular and Viral MHC Class I Molecules
Cosman, D. J., Fanger, N. A., Borges, L., Kubin, M. Z., Chin, W. A., Peterson, L., & Hsu, M.-L. (1997) · Immunity · doi:10.1016/s1074-7613(00)80529-4 · Cited by 692 · see citing works
Formation and function of the lytic NK-cell immunological synapse
Orange, J. S. (2008) · Nature reviews. Immunology · doi:10.1038/nri2381 · Cited by 613 · see citing works
Identification and characterization of circulating human transitional B cells
Sims, G. P., Ettinger, R., Shirota, Y., et al. (2005) · Blood · doi:10.1182/blood-2004-11-4284 · Cited by 604 · see citing works
Human inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G
Shiroishi, M., Tsumoto, K., Amano, K., Shirakihara, Y., Colonna, M., Braud, V. M., Allan, D., Makadzange, T., Rowland‐Jones, S., Willcox, B. E., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1431057100 · Cited by 559 · see citing works
T cell subset-specific susceptibility to aging
Czesnikiewicz-Guzik, M., Lee, W.-W., Cui, D., Hiruma, Y., Lamar, D. L., Yang, Z.-Z., Ouslander, J. G., Weyand, C. M., & Goronzy, J. J. (2008) · Clinical Immunology · doi:10.1016/j.clim.2007.12.002 · Cited by 478 · see citing works
Self-citation
Diversity of LEF/TCF action in development and disease
Arce, L., Yokoyama, N. N., & Waterman, M. L. (2006) · Oncogene · doi:10.1038/sj.onc.1210056 · Cited by 439 · see citing works
The functional consequences of alternative promoter use in mammalian genomes
Davuluri, R. V., Suzuki, Y., Sugano, S., Plass, C., & Huang, T. H.-M. (2008) · Trends in Genetics · doi:10.1016/j.tig.2008.01.008 · Cited by 408 · see citing works
The Inhibitory Receptor LIR-1 Uses a Common Binding Interaction to Recognize Class I MHC Molecules and the Viral Homolog UL18
Chapman, T. L., Heikema, A. P., & Björkman, P. J. (1999) · Immunity · doi:10.1016/s1074-7613(00)80135-1 · Cited by 366 · see citing works
Phenotypic and functional heterogeneity of human memory B cells
Sanz, I., Wei, C., Lee, F. E.-H., & Anolik, J. H. (2008) · Seminars in Immunology · doi:10.1016/j.smim.2007.12.006 · Cited by 361 · see citing works
Antigen-Receptor Signaling to Nuclear Factor κB
Schulze‐Luehrmann, J., & Ghosh, S. (2006) · Immunity · doi:10.1016/j.immuni.2006.10.010 · Cited by 330 · see citing works
Complex controls: the role of alternative promoters in mammalian genomes
Landry, J., Mager, D. L., & Wilhelm, B. T. (2003) · Trends in Genetics · doi:10.1016/j.tig.2003.09.014 · Cited by 329 · see citing works
Novel Human Transitional B Cell Populations Revealed by B Cell Depletion Therapy
Palanichamy, A., Barnard, J., Zheng, B., Owen, T., Quách, T. D., Wei, C., Looney, R. J., Sanz, I., & Anolik, J. H. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.0801859 · Cited by 300 · see citing works
T‐cell activation through immunological synapses and kinapses
Dustin, M. L. (2008) · Immunological Reviews · doi:10.1111/j.1600-065x.2008.00589.x · Cited by 283 · see citing works
Differential Expression of Leukocyte Receptor Complex-Encoded Ig-Like Receptors Correlates with the Transition from Effector to Memory CTL
Young, N. T., Uhrberg, M., Phillips, J. H., Lanier, L. L., & Parham, P. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.166.6.3933 · Cited by 177 · see citing works
Tyrosine kinases and their substrates in B lymphocytes
Kurosaki, T., & Hikida, M. (2009) · Immunological Reviews · doi:10.1111/j.1600-065x.2008.00748.x · Cited by 160 · see citing works
New Insights into the Early Molecular Events Underlying B Cell Activation
Harwood, N. E., & Batista, F. D. (2008) · Immunity · doi:10.1016/j.immuni.2008.04.007 · Cited by 158 · see citing works
The CD85/LIR-1/ILT2 Inhibitory Receptor Is Expressed by All Human T Lymphocytes and Down-Regulates Their Functions
Saverino, D., Fabbi, M., Ghiotto, F., Merlo, A. C., Bruno, S., Zarcone, D., Tenca, C., Tiso, M., Santoro, G., Anastasi, G., et al. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.165.7.3742 · Cited by 145 · see citing works
Ig-Like Transcript 2 (ILT2)/Leukocyte Ig-Like Receptor 1 (LIR1) Inhibits TCR Signaling and Actin Cytoskeleton Reorganization
Dietrich, J., Cella, M., & Colonna, M. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.166.4.2514 · Cited by 143 · see citing works
The leukocyte Ig-like receptor (LIR)-1 for the cytomegalovirus UL18 protein displays a broad specificity for different HLA class I alleles: analysis of LIR-1+ NK cell clones
Vitale, M. (1999) · International Immunology · doi:10.1093/intimm/11.1.29 · Cited by 115 · see citing works
Protein kinase Cθ, a selective upstream regulator of JNK/SAPK and IL-2 promoter activation in Jurkat T cells
Ghaffari‐Tabrizi‐Wizsy, N., Bauer, B., Villunger, A., Baier-Bitterlich, G., Altman, A., Utermann, G., Überall, F., & Baier, G. (1999) · European Journal of Immunology · doi:10.1002/(sici)1521-4141(199901)29:01 · Cited by 108 · see citing works
The inhibitory receptor LILRB1 modulates the differentiation and regulatory potential of human dendritic cells
Young, N. T., Waller, E. C. P., Patel, R., Roghanian, A., Austyn, J. M., & Trowsdale, J. (2007) · Blood · doi:10.1182/blood-2007-05-089771 · Cited by 98 · see citing works
Inhibitory Receptors CD85j, LAIR-1, and CD152 Down-Regulate Immunoglobulin and Cytokine Production by Human B Lymphocytes
Merlo, A. C., Tenca, C., Fais, F., Battini, L., Ciccone, E., Grossi, C. E., & Saverino, D. (2005) · Clinical and Vaccine Immunology · doi:10.1128/cdli.12.6.705-712.2005 · Cited by 93 · see citing works
Coupled Transcriptional and Translational Control of Cyclin-Dependent Kinase Inhibitor p18 INK4c Expression during Myogenesis
Phelps, D. E., Hsiao, K., Li, Y., Hu, N., Franklin, D. S., Westphal, E., Lee, E. Y.-H. P., & Xiong, Y. (1998) · Molecular and Cellular Biology · doi:10.1128/mcb.18.4.2334 · Cited by 86 · see citing works
Immunoglobulin-like transcripts ILT2, ILT3 and ILT7 are expressed by human dendritic cells and down-regulated following activation
Ju, X., Hacker, C., Scherer, B., et al. (2004) · Gene · doi:10.1016/j.gene.2004.02.018 · Cited by 81 · see citing works
B lymphocyte activation during cognate interactions with CD4+ T lymphocytes: molecular dynamics and immunologic consequences
Mills, D. M., & Cambier, J. C. (2003) · Seminars in Immunology · doi:10.1016/j.smim.2003.09.004 · Cited by 77 · see citing works
Expression of solute carrier 7A4 (SLC7A4) in the plasma membrane is not sufficient to mediate amino acid transport activity
Wolf, S., Janzen, A., Vékony, N., Martiné, U., Strand, D. J., & Closs, E. I. (2002) · Biochemical Journal · doi:10.1042/bj20020084 · Cited by 72 · see citing works
Dual Effect of CD85/Leukocyte Ig-Like Receptor-1/Ig-Like Transcript 2 and CD152 (CTLA-4) on Cytokine Production by Antigen-Stimulated Human T Cells
Saverino, D., Merlo, A. C., Bruno, S., Pistoia, V., Grossi, C. E., & Ciccone, E. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.168.1.207 · Cited by 70 · see citing works
Identification of the CD85 antigen as ILT2, an inhibitory MHC class I receptor of the immunoglobulin superfamily
Banham, A. H., Colonna, M., Cella, M., et al. (1999) · Journal of Leukocyte Biology · doi:10.1002/jlb.65.6.841 · Cited by 65 · see citing works
Genomic organization of the mouse peroxisome proliferator-activated receptor β/δ gene: alternative promoter usage and splicing yield transcripts exhibiting differential translational efficiency
Larsen, L. K., Amri, E., Mandrup, S., Pacot, C., & Kristiansen, K. (2002) · Biochemical Journal · doi:10.1042/bj20011821 · Cited by 50 · see citing works
CD85j (Leukocyte Ig-Like Receptor-1/Ig-Like Transcript 2) Inhibits Human Osteoclast-Associated Receptor-Mediated Activation of Human Dendritic Cells
Tenca, C., Merlo, A. C., Merck, E., Bates, E. E. M., Saverino, D., Simone, R., Zarcone, D., Trinchieri, G., Grossi, C. E., & Ciccone, E. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.174.11.6757 · Cited by 48 · see citing works
The Dialogue Between Endothelial Cells and Monocytes/Macrophages in Vascular Syndromes
Martin, J., Collot-Teixeira, S., McGregor, L., & McGregor, J. L. (2007) · Current Pharmaceutical Design · doi:10.2174/138161207780831248 · Cited by 47 · see citing works
Conserved organization of the ILT / LIR gene family within the polymorphic human leukocyte receptor complex
Young, N. T., Canavez, F., Uhrberg, M., et al. (2001) · Immunogenetics · doi:10.1007/s002510100332 · Cited by 45 · see citing works
Genomic structure, alternative maturation and tissue expression of the human BBOX1 gene
Rigault, C., Borgne, F. L., & Demarquoy, J. (2006) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2006.09.014 · Cited by 43 · see citing works
Alternative promoters and repetitive DNA elements define the species-dependent tissue-specific expression of theFMO1genes of human and mouse
Shephard, E. A., Chandan, P., Stevanovic-Walker, M., Edwards, M., & Phillips, I. R. (2007) · Biochemical Journal · doi:10.1042/bj20070523 · Cited by 38 · see citing works
Transcriptional Regulation of ILT Family Receptors
Nakajima, H., Asai, A., Okada, A., Ping, L., Hamajima, F., Sata, T., & Isobe, K. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.171.12.6611 · Cited by 37 · see citing works
Homeostatic control of B lymphocyte subsets
Crowley, J. E., Scholz, J. L., Quinn, W. J., Stadanlick, J. E., Treml, J., Treml, L. S., Hao, Y., Goenka, R., O’Neill, P. J., Matthews, A. H., et al. (2008) · Immunologic Research · doi:10.1007/s12026-008-8036-y · Cited by 35 · see citing works
Control of gcm RNA stability is necessary for proper glial cell fate acquisition
Soustelle, L., Roy, N., Ragone, G., et al. (2008) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2007.11.007 · Cited by 16 · see citing works
Short peptides sensitize target cells to CTL specific for the MHC class Ib molecule, H2-M3
Dabhi, V. M., & Fischer Lindahl, K. (1998) · European Journal of Immunology · doi:10.1002/(sici)1521-4141(199811)28:11

Flagged first, then by citations

Research works printed but not linked: 4

Fanger NA, Cosman D, Peterson L, Braddy SC, Maliszewski CR, Borges L. The MHC class I bind- ing proteins LIR-1 and LIR-2 inhibit Fc receptor- mediated signaling in monocytes. Eur J Immunol. 1998;28(11):3423-3434.
Navarro F, Llano M, Bellon T, Colonna M, Geraghty DE, Lopez-Botet M. The ILT2(LIR1) and CD94/NKG2A NK cell receptors respectively rec- ognize HLA-G1 and HLA-E molecules co-ex- pressed on target cells. Eur J Immunol. 1999; 29(1):277-283.
NK-cell immunological synapse. Nat Rev Immu- nol. 2008;8(9):713-725.
Serbina NV, Jia T, Hohl TM, Pamer EG. Mono- cyte-mediated defense against microbial patho- gens. Annu Rev Immunol. 2008;26:421-452.

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Authors: Lamar, D. L., Weyand, C. M., & Goronzy, J. J.
Affiliations: Emory University (US)
ORCID: Weyand, Cornelia M.
Goronzy, Jörg J.
Venue: Blood
Publisher: American Society of Hematology
Published: 2010-04-22
Volume: 115
Issue: 16
Pages: 3278-3286
ISSN: 0006-4971 (ISSN-L)
1528-0020
DOI: 10.1182/blood-2009-09-243493
PMC: PMC2858479
OpenAlex: W2037182265
Cited by: 45
References: 46
Citations by year: 2026: 1 · 2025: 2 · 2024: 1 · 2023: 4 · 2022: 4 · 2021: 4 · 2020: 3 · 2019: 1 · 2018: 3 · 2017: 3 · 2016: 4 · 2015: 2 · 2014: 3 · 2013: 2 · 2012: 1
FWCI: 1.37
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: Blood (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: American Foundation for Aging Research
National Institutes of Health
National Institute on Aging (R01AG015043, R01AG15043)
National Heart, Lung, and Blood Institute (R01HL117913)
National Eye Institute (R01EY011916, R01 EY 11916)
National Institute of General Medical Sciences (T32GM008169)
National Institute of Allergy and Infectious Diseases (R01AI044142, U19-AI57266, R01AI44142, R01AI108906, R56AI044142, U19AI057266)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR041974, R01 AR 41974, R01AR042527, R01AR42527)
Topics: Life Sciences › Immunology and Microbiology › Immunology › Immune Cell Function and Interaction
Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
Keywords: LILRB1, ILT2, inhibitory receptors, cell type-specific gene expression, protein synthesis inhibition, 5' untranslated region, lymphocytes, monocytes, natural killer cells, T cells, B cells, hematopoietic lineage
MeSH terms: Leukocyte Immunoglobulin-like Receptor B1, B-Lymphocytes, Base Sequence, Cell Separation, Flow Cytometry, Gene Expression Regulation, Humans, Killer Cells, Natural, Leukocytes, Mononuclear, Molecular Sequence Data, Monocytes, Promoter Regions, Genetic, Receptors, Immunologic, T-Lymphocytes, Transfection, Protein Biosynthesis, Blotting, Western, Antigens, CD, Gene Expression, Cell Lineage, Reverse Transcriptase Polymerase Chain Reaction

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Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages

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