The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Human NLRP3 inflammasome activation is Nox1-4 independent
Open accessAbstract
The NLRP3 inflammasome can be activated by pathogen-associated molecular patterns or endogenous danger-associated molecular patterns. The activation of the NLRP3 inflammasome results in proteolytic activation and secretion of cytokines of the interleukin-1 (IL-1) family. The precise mode of activation of the NLRP3 inflammasome is still elusive, but has been postulated to be mediated by reactive oxygen species (ROS) generated by an NADPH oxidase. Using primary cells from chronic granulomatous disease (CGD) patients lacking expression of p22phox, a protein that is required for the function of Nox1-4, we show that cells lacking NADPH oxidase activity are capable of secreting normal amounts of IL-1β. Thus, we provide evidence that activation of the NLRP3 inflammasome does not depend on ROS generated from an NADPH oxidase.
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“Whole blood samples [335] or isolated PBMC [335,336,337] showed no alterations in levels of active caspase-1 or IL-1β/IL18 secretion after treatment with typical inflammasome-activating stimuli, such as LPS/ATP, silica or uric acid crystals.”
From Functions of ROS in Macrophages and Antimicrobial Immunity (2021) · cites this paper as [335]
In the citing paper
“Three studies showed independently that Nox2 is completely dispensable for NLRP3 inflammasome activation in human macrophages.”
From Functions of ROS in Macrophages and Antimicrobial Immunity (2021) · cites this paper as [335]
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Cites
Thioredoxin-interacting protein links oxidative stress to inflammasome activation
Zhou, R., Tardivel, A., Thorens, B., Choi, I., & Tschopp, J. (2010) · Nature Immunology · doi:10.1038/ni.1831 · Cited by 2,708
Innate Immune Activation Through Nalp3 Inflammasome Sensing of Asbestos and Silica
Dostert, C., Pétrilli, V., Van Bruggen, R., Steele, C., Mossman, B. T., & Tschopp, J. (2008) · Science · doi:10.1126/science.1156995 · Cited by 2,658
Self-citationMitochondria and reactive oxygen species
Kowaltowski, A. J., de Souza-Pinto, N. C., Castilho, R. F., & Vercesi, A. E. (2009) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2009.05.004 · Cited by 1,162
Sumimoto, H. (2008) · FEBS Journal · doi:10.1111/j.1742-4658.2008.06488.x · Cited by 740
Malarial Hemozoin Is a Nalp3 Inflammasome Activating Danger Signal
Dostert, C., Guarda, G., Romero, J. F., Menu, P., Groß, O., Tardivel, A., Suvà, M.-L., Stehle, J., Köpf, M., Stamenkovic, I., et al. (2009) · PLoS ONE · doi:10.1371/journal.pone.0006510 · Cited by 397
Inflammasomes: guardians of cytosolic sanctity
Lamkanfi, M., & Dixit, V. M. (2008) · Immunological Reviews · doi:10.1111/j.1600-065x.2008.00730.x · Cited by 372
Intracellular Shuttling and Mitochondrial Function of Thioredoxin-interacting Protein*
Saxena, G., Chen, J.-Q., & Shalev, A. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.034421 · Cited by 310
van de Veerdonk, F. L., Smeekens, S. P., Joosten, L. A. B., Kullberg, B. J., Dinarello, C. A., van der Meer, J. W. M., & Netea, M. G. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0914795107 · Cited by 248
Natural history and early diagnosis of LAD-1/variant syndrome
Kuijpers, T. W., van Bruggen, R., Kamerbeek, N. M., et al. (2006) · Blood · doi:10.1182/blood-2006-05-021402 · Cited by 116
Self-citationThe molecular basis of chronic granulomatous disease
Meischl, C., & Roos, D. (1998) · Springer Seminars in Immunopathology · doi:10.1007/bf00792600 · Cited by 113
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The NLRP3 Inflammasome: an overview of mechanisms of activation and regulation
Kelley, N., Jeltema, D., Duan, Y., & He, Y. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20133328 · Cited by 3,539
Activation and regulation of the inflammasomes
Latz, E., Xiao, T. S., & Stutz, A. (2013) · Nature Reviews Immunology · doi:10.1038/nri3452 · Cited by 2,884
Mechanism and Regulation of NLRP3 Inflammasome Activation
He, Y., Hara, H., & Núñez, G. (2016) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2016.09.002 · Cited by 2,759
Metabolic Pathways in Immune Cell Activation and Quiescence
Pearce, E. L., & Pearce, E. J. (2013) · Immunity · doi:10.1016/j.immuni.2013.04.005 · Cited by 1,692
Molecular mechanisms regulating NLRP3 inflammasome activation
Jo, E.-K., Kim, J. K., Shin, D.-M., & Sasakawa, C. (2016) · Cellular & Molecular Immunology · doi:10.1038/cmi.2015.95 · Cited by 1,378
Metadata
- Authors
- van Bruggen, R., Köker, M. Y., Jansen, M., van Houdt, M., Roos, D., Kuijpers, T. W., & van den Berg, T. K.
- Affiliations
- Sanquin Research, and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands;
- Erciyes University (TR)
- Amsterdam UMC Location University of Amsterdam (NL)
- Emma Kinderziekenhuis (NL)
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2010-07-01
- Volume
- 115
- Issue
- 26
- Pages
- 5398-5400
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2166680226
- Cited by
- 202
- References
- 11
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 15 · 2023: 7 · 2022: 7 · 2021: 17 · 2020: 17 · 2019: 15 · 2018: 8 · 2017: 7 · 2016: 13 · 2015: 10 · 2014: 16 · 2013: 20 · 2012: 20
- FWCI
- 8.06
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Amsterdam UMC (VU Amsterdam) - Institutional Repository (publishedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Pure Amsterdam UMC (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Inflammasome and immune disorders
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Keywords
- NLRP3 inflammasome, inflammasome activation, NADPH oxidase, reactive oxygen species, chronic granulomatous disease, p22phox, interleukin-1β secretion, damage-associated molecular patterns, pathogen-associated molecular patterns
- MeSH terms
- NLR Family, Pyrin Domain-Containing 3 Protein, NADPH Oxidase 1, NADPH Oxidase 4, Carrier Proteins, Cells, Cultured, Humans, Leukocytes, Mononuclear, Reactive Oxygen Species, NADPH Oxidases, Interleukin-1beta
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