Transcytosis of HTLV-1 across a tight human epithelial barrier and infection of subepithelial dendritic cells
Open accessAbstract
Human T-cell leukemia virus type 1 (HTLV-1) is the causative agent of adult T-cell leukemia/lymphoma and HTLV-1–associated myelopathy/tropical spastic paraparesis. In addition to blood transfusion and sexual transmission, HTLV-1 is transmitted mainly through prolonged breastfeeding, and such infection represents a major risk for the development of adult T-cell leukemia/lymphoma. Although HTLV-1–infected lymphocytes can be retrieved from maternal milk, the mechanisms of HTLV-1 transmission through the digestive tract remain unknown. In the present study, we assessed HTLV-1 transport across the epithelial barrier using an in vitro model. Our results show that the integrity of the epithelial barrier was maintained during coculture with HTLV-1–infected lymphocytes, because neither morphological nor functional alterations of the cell monolayer were observed. Enterocytes were not susceptible to HTLV-1 infection, but free infectious HTLV-1 virions could cross the epithelial barrier via a transcytosis mechanism. Such virions were able to infect productively human dendritic cells located beneath the epithelial barrier. Our data indicate that HTLV-1 crosses the tight epithelial barrier without disruption or infection of the epithelium to further infect target cells such as dendritic cells. The present study provides the first data pertaining to the mode of HTLV-1 transport across a tight epithelial barrier, as can occur during mother-to-child HTLV-1 transmission during breastfeeding.
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HTLV-1, like HIV-1, crosses the epithelial barrier by transcytosis, and is captured by DCs, which can later become productively infected.
“HTLV-1, like HIV-1, crosses the epithelial barrier by transcytosis, and is captured by DCs, which can later become productively infected [73].”
From HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread (2022) · cites this paper as [73]
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Cited by
Sorted by citations
HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread
Kalinichenko, S., Komkov, D., & Mazurov, D. (2022) · Viruses · doi:10.3390/v14010152 · Cited by 68 · see citing works
“Authors have shown that both TGF-β DC and IL-4 DC were susceptible to HTLV-1 infection, whereas IFN-α DCs were resistant [72] HTLV-1, like HIV-1, crosses the epithelial barrier by transcytosis, and is captured by DCs, which can later become productively infected [73].”
Background
- Authors
- Martin-Latil, S., Gnädig, N. F., Mallet, A., Desdouits, M., Guivel-Benhassine, F., Jeannin, P., Prevost, M.-C., Schwartz, O., Gessain, A., Ozden, S., & Ceccaldi, P.-E.
- Affiliations
- Unité Epidémiologie et Physiopathologie des Virus Oncogènes, URA Centre National de la Recherche Scientifique 3015, Institut Pasteur, Paris, France;
- Centre National de la Recherche Scientifique (FR)
- Institut Pasteur (FR)
- Unité Virus et Immunité, URA Centre National de la Recherche Scientifique 3015, Institut Pasteur, Paris, France; and
- Plate Forme de Microscopie Ultrastructurale; Institut Pasteur, Paris, France;
- ORCID
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2012-07-19
- Volume
- 120
- Issue
- 3
- Pages
- 572-580
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2068793235
- Cited by
- 70
- References
- 48
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 3 · 2023: 7 · 2022: 6 · 2021: 5 · 2020: 3 · 2019: 4 · 2018: 5 · 2017: 9 · 2016: 4 · 2015: 6 · 2014: 5 · 2013: 4 · 2012: 2
- FWCI
- 2.37
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Keywords
- HTLV-1, transcytosis, epithelial barrier function, tight junctions, enterocytes, dendritic cells, mother-to-child transmission, breastfeeding, adult T-cell leukemia/lymphoma, HTLV-1-associated myelopathy/tropical spastic paraparesis
- MeSH terms
- Dendritic Cells, Epithelial Cells, Humans, T-Lymphocytes, Virion, Human T-lymphotropic virus 1, HTLV-I Infections, Coculture Techniques, Caco-2 Cells, HT29 Cells, Tight Junctions, Enterocytes, Microscopy, Electron, Transmission, HEK293 Cells, Transcytosis
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