Cited by 83Hemoglobinopathies and Related DisordersVirus-based gene therapy researchHemoglobin structure and function

Forced chromatin looping raises fetal hemoglobin in adult sickle cells to higher levels than pharmacologic inducers

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Breda, Laura, Motta, Irene, Lourenco, Silvia, Gemmo, Chiara, Deng, Wulan, Rupon, Jeremy W., Abdulmalik, Osheiza Y., Manwani, Deepa +2 more·2016·Blood

Abstract

Key Points: Ldb1 transcription factor self-association domain fused to γ-globin promoter-specific ZF protein increases HbF, reduces HbS in hSCD cells. In vitro reactivation of HbF mediated by ZF-Ldb1 exceeds pharmacologic treatment in adult hSCD cells.

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Crossref · 10.1182/blood-2016-01-691089

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  • The functional significance of the physical approximation of the promoter to the enhancer was demonstrated in experiments on the activation of transcription through the forced formation of enhancer-promoter loops.

    “The functional significance of the physical approximation of the promoter to the enhancer was demonstrated in experiments on the activation of transcription through the forced formation of enhancer-promoter loops [87,88].”

    From Enhancer Function in the 3D Genome (2023) · cites this paper as [87]

  • Stimulation of promoter activity by forced looping with an enhancer is a strong argument in favor of the loop model.

    “Stimulation of promoter activity by forced looping with an enhancer is a strong argument in favor of this model [87,88,110,111].”

    From Enhancer Function in the 3D Genome (2023) · cites this paper as [87]

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  1. PHARMACOLOGIC AND ZF-Ldb1 INDUCTION OF HbF 1143 For personal use only. on January 29, 2019. by guest www.bloodjournal.org From online July 12, 2016 originally published doi:10.1182/blood-2016-01-691089 2016 128: 1139-1143 · DOI

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Authors
Breda, L., Motta, I., Lourenco, S., Gemmo, C., Deng, W., Rupon, J. W., Abdulmalik, O. Y., Manwani, D., Blobel, G. A., & Rivella, S.
Affiliations
  • Children's Hospital of Philadelphia (US)
  • University of Milan (IT)
  • Department of Pediatrics, Division of Hematology-Oncology, Children’s Blood and Cancer Foundation Laboratories, Weill Cornell Medical College, New York, NY;
  • Weill Cornell Medicine (US)
  • Howard Hughes Medical Institute (US)
  • Albert Einstein College of Medicine (US)
Corresponding author
  • Blobel, Gerd A.
  • Rivella, Stefano
Venue
Blood
Published
2016-08-25
Volume
128
Issue
8
Pages
1139-1143
ISSN
  • 0006-4971 (ISSN-L)
  • 1528-0020
OpenAlex
W2465447473
Cited by
83
References
27
Citations by year
2026: 2 · 2025: 5 · 2024: 6 · 2023: 6 · 2022: 4 · 2021: 9 · 2020: 10 · 2019: 9 · 2018: 12 · 2017: 17 · 2016: 1 · 2015: 1
FWCI
9.43
Type
journal article
Language
en
Open access
Locations
  • Blood (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Archivio Istituzionale della Ricerca (Universita Degli Studi Di Milano) (submittedVersion, other-oa)
Funders
  • Children's Hospital of Philadelphia
  • National Institutes of Health (KL2TR000458)
  • National Heart, Lung, and Blood Institute (K01HL103186, R01HL102449, R01HL119479)
  • National Institute of Diabetes and Digestive and Kidney Diseases (R01DK084188)
  • National Center for Advancing Translational Sciences
  • FP7 Health
Topics
  • Health Sciences › Medicine › Genetics › Hemoglobinopathies and Related Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hemoglobin structure and function
Keywords
sickle cell disease, fetal hemoglobin reactivation, chromatin looping, locus control region, LDB1, zinc finger proteins, decitabine, pomalidomide, hydroxyurea, tranylcypromine, butyrate, hemoglobin S, erythroid cells, lentiviral transduction
MeSH terms
Adult, Anemia, Sickle Cell, Chromatin, DNA-Binding Proteins, Fetal Hemoglobin, Hemoglobin, Sickle, Humans, Transcription Factors, Zinc Fingers, Antigens, CD34, Erythroid Cells, LIM Domain Proteins

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