The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Superoxide-forming enzyme from human neutrophils: evidence for a flavin requirement
Open accessAbstract
The superoxide-forming activity of 27,000-g particles prepared from homogenates of zymosan-treated human neutrophils is lost if the assay is conducted in the presence of 0.045% Triton X-100. This loss in activity in the presence of detergent is prevented by 40 micron flavin adenine dinucleotide (FAD), but not by flavin mononucleotide, riboflavin, adenosine 5′-diphosphate, or adenosine 5′-monophosphate. With resting particles or particles from zymosan-treated chronic granulomatous disease neutrophils, no superoxide-forming activity is detectable even in the presence of FAD; this is true whether or not detergent is present in the assay. Particles extracted with detergent prior to assay are fully active if assayed in the presence of FAD, but show little activity if FAD is omitted from the assay mixture. These results suggest that the superoxide-forming enzyme from human neutrophils is a FAD-requiring enzyme.
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“NOX2 is predominantly expressed in phagocytes and produces a relatively large amount of superoxide in order to kill bacteria within phagosomes in an inflamed area [33,34], Figure 13.”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [33]
In the citing paper
“The transmembrane domain includes four conserved histidines that serve as binding sites for two heme moieties bound between helices III and V (288, 613), and the flavoprotein domain binds the cofactor FAD and includes a binding site for NADPH (49, 480, 613, 980).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [49]
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Citations
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 316,394
Babior, B. M., Kipnes, R. S., & Curnutte, J. T. (1973) · Journal of Clinical Investigation · doi:10.1172/jci107236 · Cited by 2,931
Self-citationThe Biochemical Basis of Phagocytosis
Sbarra, A. J., & Karnovsky, M. L. (1959) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)70011-2 · Cited by 1,256
The Reduction of Cytochrome c by Milk Xanthine Oxidase
McCord, J. M., & Fridovich, I. (1968) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)91929-0 · Cited by 1,196
Goldstein, I. M., Roos, D., Kaplan, H. B., & Weissmann, G. (1975) · Journal of Clinical Investigation · doi:10.1172/jci108191 · Cited by 746
Johnston, R. B., Keele, B. B., Misra, H. P., Lehmeyer, J. E., Webb, L., Baehner, R. L., & Rajagopalan, K. V. (1975) · Journal of Clinical Investigation · doi:10.1172/jci108055 · Cited by 704
Holmes, B. M., Page, A. R., & Good, R. A. (1967) · Journal of Clinical Investigation · doi:10.1172/jci105634 · Cited by 684
Biochemical Aspects of Phagocytosis
Iyer, G. Y. N., Islam, M. F., & Quastel, J. H. (1961) · Nature · doi:10.1038/192535a0 · Cited by 658
Root, R. K., Metcalf, J. A., Oshino, N., & Chance, B. (1975) · Journal of Clinical Investigation · doi:10.1172/jci108024 · Cited by 646
Antimicrobial mechanisms in neutrophilic polymorphonuclear leukocytes.
Sj, K. (1975) · PubMed · Cited by 573
Cited by
Sorted by citations
Oxygen-Dependent Microbial Killing by Phagocytes
BABIOR, B. M. (1978) · New England Journal of Medicine · doi:10.1056/nejm197803232981205 · Cited by 2,483
Self-citationGenetic, Biochemical, and Clinical Features of Chronic Granulomatous Disease
Segal, B. H., Leto, T. L., Gallin, J. I., Malech, H. L., & Holland, S. M. (2000) · Medicine · doi:10.1097/00005792-200005000-00004 · Cited by 887
The superoxide-generating NADPH oxidase: structural aspects and activation mechanism
Vignais, P. V. (2002) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-002-8520-9 · Cited by 799
The O2−-forming NADPH oxidase of the phagocytes: nature, mechanisms of activation and function
Rossi, F. (1986) · Biochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics · doi:10.1016/0304-4173(86)90005-4 · Cited by 656
The superoxide‐generating oxidase of phagocytic cells
Morel, F., Doussière, J., & Vignais, P. V. (1991) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1991.tb16312.x · Cited by 565
Metadata
- Authors
- Babior, B., & Kipnes, R.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 1977-09-01
- Volume
- 50
- Issue
- 3
- Pages
- 517-524
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2475048516
- Cited by
- 174
- References
- 23
- Citations by year
- 2023: 4 · 2022: 2 · 2021: 2 · 2019: 3 · 2018: 1 · 2016: 1 · 2014: 2 · 2013: 1
- FWCI
- 2.86
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Bioactive Compounds and Antitumor Agents
- Keywords
- neutrophils, flavin adenine dinucleotide, NADPH oxidase, zymosan, chronic granulomatous disease, detergent extraction, Triton X-100, flavin mononucleotide, riboflavin
- MeSH terms
- Adenine Nucleotides, Detergents, Flavins, Granulomatous Disease, Chronic, Humans, Neutrophils, Peroxides, Zymosan
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