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In vitro tumor cell cytolysis mediated by peptide defensins of human and rabbit granulocytes
Open accessAbstract
We examined the activity of defensins, cysteine-rich cationic peptides that are abundant in the cytoplasmic granules of human and rabbit granulocytes, against various tumor targets. The three human defensins, HNP-1, HNP-2, and HNP-3, lysed human and murine targets in chromium release and dye exclusion assays. Defensin-mediated tumor cell lysis was concentration-dependent, inhibited by serum, and dependent on temperature-sensitive events. Lysis was first detected by three hours of incubation and it reached a plateau between eight and 14 hours. In vitro exposure of murine teratocarcinoma cells to HNP 1–3 abrogated their oncogenicity in vivo. Nonmalignant target cells were also susceptible to defensin-mediated lysis. Four rabbit granulocyte defensins exerted marked (NP-1, NP-2) or moderate (NP-3a, NP-3b) cytotoxic activity, whereas defensin NP-5 was not cytotoxic. When tumor cells were incubated with human defensins in combination with hydrogen peroxide, synergistic cytotoxicity was detected. As defensins are released from granulocytes by various stimuli, their release could contribute to extracellular cytotoxicity which is independent of reactive oxygen intermediates.
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Cited by
Defensins: antimicrobial peptides of innate immunity
Ganz, T. (2003) · Nature Reviews Immunology · doi:10.1038/nri1180 · Cited by 3,066
Self-citationT he D iagnostic A pplications of S aliva — A R eview
Kaufman, E., & Lamster, I. B. (2002) · Critical Reviews in Oral Biology & Medicine · doi:10.1177/154411130201300209 · Cited by 887
The Use of Therapeutic Peptides to Target and to Kill Cancer Cells
J. Boohaker, R., W. Lee, M., Vishnubhotla, P., M. Perez, J. L., & R. Khaled, A. (2012) · Current Medicinal Chemistry · doi:10.2174/092986712801661004 · Cited by 302
Metadata
- Authors
- Lichtenstein, A., Ganz, T., Selsted, M., & Lehrer, R.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 1986-12-01
- Volume
- 68
- Issue
- 6
- Pages
- 1407-1410
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W1629982671
- Cited by
- 263
- References
- 0
- FWCI
- 6.03
- Type
- journal article
- Language
- en
- Open access
- bronze
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