The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Desferrioxamine induces erythropoietin gene expression and hypoxia- inducible factor 1 DNA-binding activity: implications for models of hypoxia signal transduction
Open accessAbstract
Erythropoietin (EPO) gene transcription is activated in kidney cells in vivo and in Hep3B cells exposed to hypoxia or cobalt chloride. Hypoxia- inducible factor 1 (HIF-1) is a nuclear factor that binds to the hypoxia-inducible enhancer of the EPO gene at a site that is required for transcriptional activation. HIF-1 DNA-binding activity is induced by hypoxia or cobalt chloride treatment of Hep3B cells. We report that treatment of Hep3B cells with desferrioxamine (DFX) induced HIF-1 activity and EPO RNA expression with kinetics similar to the induction of HIF-1 by hypoxia or cobalt chloride. Induction by each of these stimuli was inhibited by cycloheximide, indicating a requirement for de novo protein synthesis. DFX appears to induce HIF-1 by chelating iron as induction was inhibited by coadministration of ferrous ammonium sulfate. DFX administration to mice transiently increased EPO RNA levels in the kidney. As previously shown for hypoxia and cobalt treatment, DFX also induced HIF-1 activity in non-EPO-producing cells, suggesting the existence of a common hypoxia signal-transduction pathway leading to HIF-1 induction in different cell types.
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“HIF stabilization can be induced by iron chelators including desferrioxamine and bacterial siderophores [62, 63].”
From Hypoxia and hypoxia-inducible factors as regulators of T cell development, differentiation, and function (2013) · cites this paper as [62]
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Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,515
Semenza, G. L., & Wang, G. L. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.12.5447 · Cited by 2,617
Self-citationGeneral involvement of hypoxia-inducible factor 1 in transcriptional response to hypoxia.
Wang, G. L., & Semenza, G. L. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.9.4304 · Cited by 1,468
Self-citationErythropoietin: structure, control of production, and function
JELKMANN, W. E. B. (1992) · Physiological Reviews · doi:10.1152/physrev.1992.72.2.449 · Cited by 1,167
Regulation of the Erythropoietin Gene: Evidence That the Oxygen Sensor Is a Heme Protein
Goldberg, M. A., Dunning, S. P., & Bunn, H. F. (1988) · Science · doi:10.1126/science.2849206 · Cited by 1,032
Semenza, G. L., Nejfelt, M. K., Chi, S. M., & Antonarakis, S. E. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.13.5680 · Cited by 990
Self-citationCharacterization of hypoxia-inducible factor 1 and regulation of DNA binding activity by hypoxia.
Wang, G. L., & Semenza, G. L. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)80571-7 · Cited by 863
Self-citationA haemoprotein with kinase activity encoded by the oxygen sensor of Rhizobium meliloti
Gilles‐Gonzalez, M., Ditta, G. S., & Helinski, D. R. (1991) · Nature · doi:10.1038/350170a0 · Cited by 477
Localization of erythropoietin synthesizing cells in murine kidneys by in situ hybridization
Koury, S., Bondurant, M. C., & Koury, M. (1988) · Blood · doi:10.1182/blood.v71.2.524.524 · Cited by 469
Peritubular cells are the site of erythropoietin synthesis in the murine hypoxic kidney.
Lacombe, C., Silva, J. L. D., Bruneval, P., Fournier, J. G., Wendling, F., Casadevall, N., Camilleri, J.-P., Bariety, J., Varet, B. R., & Tambourin, P. E. (1988) · Journal of Clinical Investigation · doi:10.1172/jci113363 · Cited by 437
Cascade regulation of nif gene expression in Rhizobium meliloti
David, M., Daveran‐Mingot, M., Batut, J., Dedieu, A., Domergue, O., Ghai, J., Hertig, C. M., Boistard, P., & Kahn, D. (1988) · Cell · doi:10.1016/s0092-8674(88)80012-6 · Cited by 409
Rapid and quantitative preparation of cytoplasmic RNA from small numbers of cells
Gough, N. M. (1988) · Analytical Biochemistry · doi:10.1016/0003-2697(88)90164-9 · Cited by 403
The regulated expression of erythropoietin by two human hepatoma cell lines.
Goldberg, M. A., Glass, G. A., Cunningham, J. M., & Bunn, H. F. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.22.7972 · Cited by 358
Pugh, C. W., Tan, C., Jones, R. W., & Ratcliffe, P. J. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.23.10553 · Cited by 255
Koury, S. T., Koury, M., Bondurant, M. C., Caro, J., & Gräber, S. E. (1989) · Blood · doi:10.1182/blood.v74.2.645.645 · Cited by 252
Semenza, G. L., Koury, S. T., Nejfelt, M. K., Gearhart, J. D., & Antonarakis, S. E. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.19.8725 · Cited by 248
Self-citationBlanchard, K. L., Acquaviva, A. M., Galson, D. L., & Bunn, H. F. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.12.5373 · Cited by 215
Beck, I., Ramirez, S., Weinmann, R. S., & Caro, J. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)98438-3 · Cited by 206
Goldberg, M., Gaut, C., & Bunn, H. F. (1991) · Blood · doi:10.1182/blood.v77.2.271.271 · Cited by 199
Stimulation of erythropoietin gene transcription during hypoxia and cobalt exposure
Schuster, S. J., Badiavas, E. V., Costa-Giomi, P., Weinmann, R. S., Erslev, A. J., & Caro, J. (1989) · Blood · doi:10.1182/blood.v73.1.13.13 · Cited by 174
Physiologic regulation and tissue localization of renal erythropoietin messenger RNA
Schuster, S. J., Wilson, J., Erslev, A. J., & Caro, J. (1987) · Blood · doi:10.1182/blood.v70.1.316.316 · Cited by 171
Monson, E. K., Weinstein, M. B., Ditta, G. S., & Helinski, D. R. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.10.4280 · Cited by 163
Polycythemia in transgenic mice expressing the human erythropoietin gene.
Semenza, G. L., Traystman, M. D., Gearhart, J. D., & Antonarakis, S. E. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.7.2301 · Cited by 131
Self-citationMadan, A., & Curtin, P. T. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.9.3928 · Cited by 129
Semenza, G. L., Dureza, R. C., Traystman, M. D., Gearhart, J. D., & Antonarakis, S. E. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.3.930 · Cited by 119
Self-citationRutherford, T. R., & Weatherall, D. J. (1979) · Cell · doi:10.1016/0092-8674(79)90017-5 · Cited by 107
Keng, T. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.6.2616-2623.1992 · Cited by 101
Inverse Regulation of the Yeast COX5 Genes by Oxygen and Heme
Hodge, M. R., Kim, G. A., Singh, K. K., & Cumsky, M. G. (1989) · Molecular and Cellular Biology · doi:10.1128/mcb.9.5.1958 · Cited by 87
Decline of erythropoietin formation at continuous hypoxia is not due to feedback inhibition
Eckardt, K., Dittmer, J., Neumann, R., Bauer, C., & Kurtz, A. (1990) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.1990.258.5.f1432 · Cited by 67
Regulatory elements of the erythropoietin gene
Imagawa, S., Goldberg, M., Doweiko, J. P., & Bunn, H. F. (1991) · Blood · doi:10.1182/blood.v77.2.278.278 · Cited by 64
Oxygen regulation of nifA transcription in vitro.
Agron, P. G., Ditta, G. S., & Helinski, D. R. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.8.3506 · Cited by 63
Enhancement by hypoxia of human erythropoietin gene transcription in vitro.
Costa-Giomi, P., Caro, J., & Weinmann, R. S. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)86926-5 · Cited by 56
Localization of erythropoietin synthesizing cells in murine kidneys by in situ hybridization
Koury, S., Bondurant, M. C., & Koury, M. (1988) · Blood · doi:10.1182/blood.v71.2.524.bloodjournal712524 · Cited by 36
In vivo and in vitro effects of 3-hydroxypyridin-4-one chelators on murine hemopoiesis.
Hoyes, K. P., Jones, H. M., Abeysinghe, R. D., Hider, R. C., & Porter, J. B. (1993) · PubMed · Cited by 23
Koury, S., Koury, M., Bondurant, M. C., et al. (1989) · Blood · doi:10.1182/blood.v74.2.645.bloodjournal742645 · Cited by 21
Physiologic regulation and tissue localization of renal erythropoietin messenger RNA
Schuster, S. J., Wilson, J., Erslev, A. J., et al. (1987) · Blood · doi:10.1182/blood.v70.1.316.bloodjournal701316 · Cited by 15
Regulatory elements of the erythropoietin gene
Imagawa, S., Goldberg, M., Doweiko, J. P., et al. (1991) · Blood · doi:10.1182/blood.v77.2.278.bloodjournal772278 · Cited by 4
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The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis
Maxwell, P. H., Wiesener, M. S., Chang, G.-W., Clifford, S. C., Vaux, E. C., Cockman, M. E., Wykoff, C. C., Pugh, C. W., Maher, E. R., & Ratcliffe, P. J. (1999) · Nature · doi:10.1038/20459 · Cited by 5,180
Activation of vascular endothelial growth factor gene transcription by hypoxia-inducible factor 1
Forsythe, J. A., Jiang, B.-H., Iyer, N. V., Agani, F., Leung, S. W., Koos, R. D., & Semenza, G. L. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.9.4604 · Cited by 3,981
Self-citationHuang, L. E., Gu, J., Schau, M., & Bunn, H. F. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.14.7987 · Cited by 2,113
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Wang, G. L., & Semenza, G. L. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.3.1230 · Cited by 2,094
Self-citation
Metadata
- Authors
- Wang, G., & Semenza, G.
- Affiliations
- Johns Hopkins University (US)
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 1993-12-15
- Volume
- 82
- Issue
- 12
- Pages
- 3610-3615
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W1545825939
- Cited by
- 766
- References
- 33
- Citations by year
- 2026: 4 · 2025: 9 · 2024: 12 · 2023: 24 · 2022: 18 · 2021: 26 · 2020: 24 · 2019: 18 · 2018: 19 · 2017: 17 · 2016: 34 · 2015: 24 · 2014: 26 · 2013: 26 · 2012: 32
- FWCI
- 5.23
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01dk039869-16)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK 39869)
- Topics
- Health Sciences › Medicine › Hematology › Erythropoietin and Anemia Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Hemoglobin structure and function
- Keywords
- deferoxamine, erythropoietin gene expression, HIF-1, iron chelation, cobalt chloride, Hep3B cells, ferrous ammonium sulfate, cycloheximide, de novo protein synthesis
- MeSH terms
- Base Sequence, Cell Line, Cell Nucleus, Cobalt, Deferoxamine, DNA-Binding Proteins, Erythropoietin, Humans, Liver Neoplasms, Molecular Sequence Data, Nuclear Proteins, RNA, Neoplasm, Transcription Factors, Tumor Cells, Cultured, Oligonucleotide Probes, Signal Transduction, Cell Hypoxia, Gene Expression, Hepatoblastoma, Hypoxia-Inducible Factor 1, Hypoxia-Inducible Factor 1, alpha Subunit
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