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Cited by 91Viral-associated cancers and disordersCytomegalovirus and herpesvirus researchHerpesvirus Infections and Treatments

Patterns of cytokine gene expression in infectious mononucleosis

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Foss, HD, Herbst, H, Hummel, M, Araujo, I, Latza, U, Rancso, C, Dallenbach, F, Stein, H·1994·Blood
DOI 10.1182/blood.v83.3.707.707
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Abstract

Primary infection with Epstein-Barr virus (EBV) may arise as infectious mononucleosis (IM) in adolescents and young adults. Morphologically, IM- affected lymphoid tissue is characterized by expanded interfollicular areas with formation of atypical lymphoid blasts. It is assumed that morphology and clinical presentation of IM are related to characteristic patterns of cytokine production by EBV-infected and reactive cells. We studied IM tonsils of eight patients and six normal tonsils with a double in situ hybridization procedure using [35S]- labeled RNA probes specific for various cytokines and digoxigenin- labeled probes for the detection of the nuclear EBV encoded RNA transcripts, EBER 1 and 2. All of the IM cases displayed the same distinct cytokine gene expression pattern. When compared with interfollicular areas of normal tonsils, expression of lymphotoxin (LT), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and IL-1 beta, but not IL-8 or IL-1 alpha was strongly enhanced in interfollicular areas in IM tonsils. LT was expressed predominantly by EBV-infected cells. TNF-alpha transcripts were also present in EBV- infected cells, although in smaller proportions. IL-6 specific signals were only found in few EBV-infected cells. IL-1 alpha-, IL-1 beta-, and IL-8-specific signals were not observed in EBV-infected cells, but were present at high signal intensity in many cells within and around foci of EBV-infected cells (IL-1 beta), next to areas of necrosis (IL-8, IL- 1 beta), or in epithelial cells (IL-1 alpha). These data suggest that EBV infection in form of IM results in induction of specific sets of cytokine genes in EBV-infected and in neighboring EBV-negative cells contributing to the characteristic morphology and cellular arrangement of the lesion as well as the clinical presentation.

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  • Inflammatory cytokines IL-1 and TNF appear to be likely candidates in mediating sleep changes observed in HIV infection, given their sleep-modulating role in animal models and upregulation in HIV-infected patients.

    “Inflammatory cytokines (e.g., IL-1 and TNF) appear to be likely candidates in mediating sleep changes observed in HIV infection, given their sleep modulating role in animal models (see sect. IIB1) and upregulation in HIV-infected patients (195, 477, 540).”

    From The Sleep-Immune Crosstalk in Health and Disease (2019) · cites this paper as [195]

  • Some studies reported an association between sleep parameters (SWA, sleep duration) and pro-inflammatory cytokine dysregulation in HIV-infected individuals.

    “some of the very few studies conducted in this area reported an association between sleep parameters (i.e., SWA, sleep duration) and pro-inflammatory cytokine dysregulation in HIV-infected individuals (133, 195).”

    From The Sleep-Immune Crosstalk in Health and Disease (2019) · cites this paper as [195]

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Authors
Foss, H., Herbst, H., Hummel, M., Araujo, I., Latza, U., Rancso, C., Dallenbach, F., & Stein, H.
Affiliations
  • Freie Universität Berlin (DE)
  • Institute of Pathology, Klinikum Steglitz, Free University of Berlin, Germany.
ORCID
Hummel, M
Venue
Blood
Publisher
American Society of Hematology
Published
1994-02-01
Volume
83
Issue
3
Pages
707-712
ISSN
  • 0006-4971 (ISSN-L)
  • 1528-0020
DOI
10.1182/blood.v83.3.707.707
OpenAlex
W1264625845
Cited by
91
References
34
Citations by year
2025: 2 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 1 · 2016: 1 · 2015: 4 · 2014: 3 · 2012: 2
FWCI
3.00
Type
journal article
Language
en
Open access
Locations
  • Blood (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Oncology › Viral-associated cancers and disorders
  • Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
  • Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
Keywords
infectious mononucleosis, Epstein-Barr virus, cytokine gene expression, tonsil, lymphotoxin, TNF-α, interleukin-6, IL-1β, interleukin-8, lymphoid tissue morphology
MeSH terms
Herpesvirus 4, Human, Gene Expression Regulation, Humans, Infectious Mononucleosis, Interleukin-1, RNA, Small Nuclear, RNA, Viral, Tumor Necrosis Factor-alpha, Interleukin-6, Cytokines

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