The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Predominant role of catalase in the disposal of hydrogen peroxide within human erythrocytes
Open accessAbstract
Purified enzymes were mixed to form a cell-free system that simulated the conditions for removal of hydrogen peroxide within human erythrocytes. Human glutathione peroxidase disposed of hydrogen peroxide (H2O2) at a rate that was only 17% of the rate at which human catalase simultaneously removed hydrogen peroxide. The relative rates observed were in agreement with the relative rates predicted from the kinetic constants of the two enzymes. These results confirm two earlier studies on intact erythrocytes, which refuted the notion that glutathione peroxidase is the primary enzyme for removal of hydrogen peroxide within erythrocytes. The present findings differ from the results with intact cells, however, in showing that glutathione peroxidase accounts for even less than 50% of the removal of hydrogen peroxide. A means is proposed for calculating the relative contribution of glutathione peroxidase and catalase in other cells and species. The present results raise the possibility that the major function of glutathione peroxidase may be the disposal of organic peroxides rather than the removal of hydrogen peroxide.
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“they have an increased level of antioxidant enzymes and enhanced glucose flux into the pentose phosphate pathway (ppp)60,61 to generate more NADPH. In a normal setting, this protects erythrocytes from high-dose ascorbate.”
From Targeting cancer vulnerabilities with high-dose vitamin C (2019) · cites this paper as [60]
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Wendel, A. (1981) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(81)77046-0 · Cited by 3,032
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Glutathione Peroxidase: The Primary Agent for the Elimination of Hydrogen Peroxide in Erythrocytes*
Cohen, G., & Hochstein, P. (1963) · Biochemistry · doi:10.1021/bi00906a038 · Cited by 666
Catalase: a tetrameric enzyme with four tightly bound molecules of NADPH.
Kirkman, H., & Gaetani, G. F. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.14.4343 · Cited by 476
Self-citationAn Improved Coupled Test Procedure for Glutathione Peroxidase (EC 1.11.1.9.) in Blood
Günzler, W. A., Kremers, H., & Flohé, L. (1974) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm.1974.12.10.444 · Cited by 369
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Research works printed but not linked: 1
- Aebi H, Suter H: Protective function of reduced glutathione (G-SH) against the effect of peroxidative substances and of irradia- tion in the red cell, in FlohC L, Benohr H, Sies H, Waller HD, Wendell A (eds): Glutathione. Stuttgart, Germany, Thieme, 1974, p 198
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Role of Catalase in Oxidative Stress- and Age-Associated Degenerative Diseases
Nandi, A., Yan, L.-J., Jana, C. K., & Das, N. (2019) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2019/9613090 · Cited by 1,185
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Chen, Q., Espey, M. G., Sun, A. Y., Lee, J.-H., Krishna, M. C., Shacter, E., Choyke, P. L., Pooput, C., Kirk, K. L., Buettner, G. R., et al. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0702854104 · Cited by 676
Potential Theranostics Application of Bio-Synthesized Silver Nanoparticles (4-in-1 System)
Mukherjee, S., Chowdhury, D., Kotcherlakota, R., Patra, S., Vinothkumar, B., Bhadra, M. P., Sreedhar, B., & Patra, C. R. (2014) · Theranostics · doi:10.7150/thno.7819 · Cited by 512
Metadata
- Authors
- Gaetani, G., Ferraris, A., Rolfo, M., Mangerini, R., Arena, S., & Kirkman, H.
- Affiliations
- University of Genoa (IT)
- Division of Hematological Oncology, Istituto Nazionale per la Ricerca sul Cancro, University of Genoa, Italy.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 1996-02-15
- Volume
- 87
- Issue
- 4
- Pages
- 1595-1599
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W160949418
- Cited by
- 296
- References
- 21
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 12 · 2023: 12 · 2022: 13 · 2021: 11 · 2020: 15 · 2019: 23 · 2018: 14 · 2017: 13 · 2016: 15 · 2015: 17 · 2014: 25 · 2013: 15 · 2012: 17
- FWCI
- 1.64
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glutathione Transferases and Polymorphisms
- Keywords
- catalase, glutathione peroxidase, hydrogen peroxide, erythrocytes, cell-free system, enzyme kinetics, antioxidant enzymes
- MeSH terms
- Catalase, Cell-Free System, Erythrocytes, Gluconates, Glutathione, Glutathione Peroxidase, Humans, Hydrogen Peroxide, NAD
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