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STAT5 activation contributes to growth and viability in Bcr/Abl-transformed cells

Sillaber, Christian, Gesbert, Franck, Frank, David A., Sattler, Martin, Griffin, James D.·2000·Blood
DOI 10.1182/blood.v95.6.2118
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Abstract

The transcription factor STAT5 is constitutively tyrosine phosphorylated and activated after transformation of hematopoietic cells by p210Bcr/Abl. A truncated form of STAT5B (▵STAT5; aa, 1-683) that lacks tyrosine 699 and the transcriptional activation domain was introduced into Ba/F3p210 cells under the control of a tetracycline-inducible promoter. Treatment of these cells with doxycycline, a tetracycline analogue, induced expression of ▵STAT5 and inhibited STAT5-dependent transcription. ▵STAT5 coprecipitated with STAT5 and decreased Bcr/Abl-dependent tyrosine phosphorylation of endogenous STAT5. Induction of ▵STAT5 inhibited growth of Ba/F3p210 cells (26%-52% of control levels at 4 days) but did not cause cell-cycle arrest. ▵STAT5 reduced viability of Ba/F3p210 cells and increased sensitivity of the cells to the cytotoxic drugs hydroxyurea and cytarabine. These results indicate that high-level expression of ▵STAT5, as achieved here by using a tetracycline-inducible promoter, inhibits STAT5 activity, reduces the growth rate of Ba/F3p210 cells by inhibiting viability, and results in increased sensitivity to chemotherapeutic drugs. It is therefore likely that STAT5 activation plays a role in the transformation of hematopoietic cell lines by p210Bcr/Abl.

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Research works printed but not linked: 46

  1. Nowell, 1960, A minute chromosome in human chronic granulocytic leukemia., Science., 132, 1497
  2. Konopka, 1985, Detection of c-abl tyrosine kinase activity in vitro permits direct comparison of normal and altered abl products., Mol Cell Biol., 5, 3116, 10.1128/MCB.5.11.3116 · DOI
  3. Pendergast, 1993, BCR-ABL-induced oncogenesis is mediated by direct interaction with the SH2 domain of the GRB-2 adaptor protein., Cell., 75, 175, 10.1016/S0092-8674(05)80094-7 · DOI
  4. Mandanas, 1993, Role of p21 RAS in p210 bcr-abl transformation of murine myeloid cells., Blood., 82, 1838, 10.1182/blood.V82.6.1838.1838 · DOI
  5. Skorski, 1997, Transformation of hematopoietic cells by BCR/ABL requires activation of a PI-3k/Akt-dependent pathway., EMBO J., 16, 6151, 10.1093/emboj/16.20.6151 · DOI · Paper page
  6. Skorski, 1998, The SH3 domain contributes to BCR/ABL-dependent leukemogenesis in vivo: role in adhesion, invasion, and homing., Blood., 91, 406, 10.1182/blood.V91.2.406 · DOI
  7. Carlesso, 1996, Tyrosyl phosphorylation and DNA binding activity of signal transducers and activators of transcription (STAT) proteins in hematopoietic cell lines transformed by Bcr/Abl., J Exp Med., 183, 811, 10.1084/jem.183.3.811 · DOI
  8. Ilaria, 1996, P210 and P190 (BCR/ABL) induce the tyrosine phosphorylation and DNA binding activity of multiple specific STAT family members., J Biol Chem., 271, 31,704, 10.1074/jbc.271.49.31704 · DOI
  9. Mui, 1995, Interleukin-3, granulocyte-macrophage colony stimulating factor and interleukin-5 transduce signals through two STAT5 homologs., EMBO J., 14, 1166, 10.1002/j.1460-2075.1995.tb07100.x · DOI
  10. Schwaller, 1998, Transformation of hematopoietic cell lines to growth-factor independence and induction of a fatal myelo- and lymphoproliferative disease in mice by retrovirally transduced TEL/JAK2 fusion genes., EMBO J., 17, 5321, 10.1093/emboj/17.18.5321 · DOI
  11. Okuda, 1996, p210BCR/ABL, p190BCR/ABL, and TEL/ABL activate similar signal transduction pathways in hematopoietic cell lines., Oncogene., 13, 1147
  12. Shuai, 1994, Interferon activation of the transcription factor Stat91 involves dimerization through SH2-phosphotyrosyl peptide interactions., Cell., 76, 821, 10.1016/0092-8674(94)90357-3 · DOI · Paper page
  13. Morrigl, 1996, Deletion of the carboxy-terminal transactivation domain of MGF-STAT5 results in sustained DNA-binding and a dominant negative phenotype., Mol Cell Biol., 16, 5691, 10.1128/MCB.16.10.5691 · DOI
  14. Matsumoto, 1997, CIS, a cytokine inducible SH2 protein, is a target of the JAK-STAT5 pathway and modulates STAT5 activation., Blood., 89, 3148, 10.1182/blood.V89.9.3148 · DOI
  15. Yoshimura, 1996, Mouse oncostatin M: an immediate early gene induced by multiple cytokines through the JAK-STAT5 pathway., EMBO J., 15, 1055, 10.1002/j.1460-2075.1996.tb00443.x · DOI
  16. Socolovsky, 1999, Fetal anemia and apoptosis of red-cell progenitors in Stat5a−/−5b−/− mice: a direct role for Stat5 in Bcl-X(L) induction., Cell., 98, 181, 10.1016/S0092-8674(00)81013-2 · DOI
  17. Teglund, 1998, Stat5a and Stat5b proteins have essential and nonessential, or redundant, roles in cytokine responses., Cell., 93, 841, 10.1016/S0092-8674(00)81444-0 · DOI
  18. Mui, 1996, Suppression of interleukin-3-induced gene expression by a C-terminal truncated Stat5: role of Stat5 in proliferation., EMBO J., 15, 2425, 10.1002/j.1460-2075.1996.tb00600.x · DOI
  19. Klucher, 1998, Secondary mutation maintains the transformed state in BaF3 cells with inducible BCR/ABL expression., Blood., 91, 3927, 10.1182/blood.V91.10.3927.3927_3927_3934 · DOI
  20. Daley, 1990, Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome., Science., 247, 824, 10.1126/science.2406902 · DOI · Paper page
  21. Jaster, 1997, Jak2 is required for induction of the murine DUB-1 gene., Mol Cell Biol., 17, 3364, 10.1128/MCB.17.6.3364 · DOI
  22. Daley, 1988, Transformation of an interleukin 3-dependent hematopoietic cell line by the chronic myelogenous leukemia-specific P210bcr/abl protein., Proc Natl Acad Sci U S A., 85, 9312, 10.1073/pnas.85.23.9312 · DOI
  23. Druker, 1996, Effects of a selective inhibitor of the Abl tyrosine kinase on the growth of Bcr-Abl positive cells., Nat Med., 2, 561, 10.1038/nm0596-561 · DOI · Paper page
  24. Boucheron, 1998, A single amino acid in the DNA binding regions of STAT5a and STAT5b confers distinct DNA binding specificities., J Biol Chem., 273, 33,936, 10.1074/jbc.273.51.33936 · DOI
  25. Yoshimura, 1995, A novel cytokine-inducible gene encodes an SH2-containing protein that binds to tyrosine-phosphorylated interleukin 3 and erythropoietin receptors., EMBO J., 14, 2816, 10.1002/j.1460-2075.1995.tb07281.x · DOI
  26. Verdier, 1998, A sequence of the CIS gene promoter interacts preferentially with two associated STAT5A dimers: a distinct biochemical difference between STAT5A and STAT5B., Mol Cell Biol., 18, 5852, 10.1128/MCB.18.10.5852 · DOI
  27. Verdier, 1998, Proteasomes regulate erythropoietin receptor and signal transducer and activator of transcription 5 (STAT5) activation: possible involvement of the ubiquitinated Cis protein., J Biol Chem., 273, 28,185, 10.1074/jbc.273.43.28185 · DOI
  28. Nosaka, 1999, STAT5 as a molecular regulator of proliferation, differentiation and apoptosis in hematopoietic cells., EMBO J., 18, 4754, 10.1093/emboj/18.17.4754 · DOI
  29. Verhoven, 1995, Mechanism of phosphatidylserine exposure, a phagocyte recognition signal, on apoptotic T lymphocytes., J Exp Med., 182, 1597, 10.1084/jem.182.5.1597 · DOI
  30. Klampfer, 1999, Sodium salicylate activates caspases and induces apoptosis of myeloid leukemia cell lines., Blood., 93, 2386, 10.1182/blood.V93.7.2386 · DOI
  31. Nieborowska-Skorska, 1999, Signal transducer and activator of transcription (STAT)5 activation by BCR/ABL is dependent on intact Src homology (SH)3 and SH2 domains of BCR/ABL and is required for leukemogenesis., J Exp Med., 189, 1229, 10.1084/jem.189.8.1229 · DOI
  32. Bedi, 1994, Inhibition of apoptosis by BCR-ABL in chronic myeloid leukemia., Blood., 83, 2038, 10.1182/blood.V83.8.2038.2038 · DOI
  33. Gordon, 1987, Altered adhesive interactions with marrow stroma of haematopoietic progenitor cells in chronic myeloid leukaemia., Nature., 328, 342, 10.1038/328342a0 · DOI
  34. Salgia, 1997, BCR/ABL induces multiple abnormalities of cytoskeletal function., J Clin Invest., 100, 46, 10.1172/JCI119520 · DOI · Paper page
  35. Albrecht, 1996, Primary proliferating immature myeloid cells from CML patients are not resistant to induction of apoptosis by DNA damage and growth factor withdrawal., Br J Haematol., 95, 501, 10.1046/j.1365-2141.1996.d01-1934.x · DOI
  36. Amos, 1995, Apoptosis in chronic myeloid leukaemia: normal responses by progenitor cells to growth factor deprivation, X-irradiation and glucocorticoids., Br J Haematol., 91, 387, 10.1111/j.1365-2141.1995.tb05308.x · DOI
  37. Jiang, 1998, Autonomous multi-lineage differentiation in vitro of primitive PH+ CD34+ cells from patients with chronic myelogenous leukemia (CML) [abstract]., Blood., 92, 1041a
  38. Tauchi, 1994, SH2-containing phosphotyrosine phosphatase Syp is a target of p210bcr-abl tyrosine kinase., J Biol Chem., 269, 15,381, 10.1016/S0021-9258(17)36617-6 · DOI
  39. Chen, 1995, Direction of p21ras-generated signals towards cell growth or apoptosis is determined by protein kinase C and Bcl-2., Oncogene., 11, 1487
  40. Kinoshita, 1995, Regulation of Bcl-2 expression by oncogenic Ras protein in hematopoietic cells., Oncogene., 10, 2207
  41. Amarante-Mendes, 1998, Bcl-2-independent Bcr-Abl-mediated resistance to apoptosis: protection is correlated with up regulation of Bcl-XL., Oncogene., 16, 1383, 10.1038/sj.onc.1201664 · DOI
  42. Onishi, 1998, Identification and characterization of a constitutively active STAT5 mutant that promotes cell proliferation., Mol Cell Biol., 18, 3871, 10.1128/MCB.18.7.3871 · DOI
  43. Nishinakamura, 1996, Hematopoiesis in mice lacking the entire granulocyte-macrophage colony-stimulating factor/interleukin-3/interleukin-5 functions., Blood., 88, 2458, 10.1182/blood.V88.7.2458.bloodjournal8872458 · DOI
  44. Wong, 1989, Retrovirus-mediated transfer and expression of the interleukin-3 gene in mouse hematopoietic cells result in a myeloproliferative disorder., Mol Cell Biol., 9, 798, 10.1128/MCB.9.2.798 · DOI
  45. Lang, 1987, Transgenic mice expressing a hemopoietic growth factor gene (GM-CSF) develop accumulations of macrophages, blindness, and a fatal syndrome of tissue damage., Cell., 51, 675, 10.1016/0092-8674(87)90136-X · DOI
  46. D'Andrea, 1998, Dysregulated hematopoiesis and a progressive neurological disorder induced by expression of an activated form of the human common beta chain in transgenic mice., J Clin Invest., 102, 1951, 10.1172/JCI3729 · DOI

Cited by

Metadata

Authors
Sillaber, C., Gesbert, F., Frank, D. A., Sattler, M., & Griffin, J. D.
Affiliations
From the Department of Adult Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA.
Venue
Blood
Publisher
American Society of Hematology
Published
2000-03-15
Volume
95
Issue
6
Pages
2118-2125
ISSN
  • 1528-0020
  • 0006-4971
DOI
10.1182/blood.v95.6.2118
Cited by
198
References
0
Language
en

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