CoChat
Sign Up
Cited by 607

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Blood-derived nurse-like cells protect chronic lymphocytic leukemia B cells from spontaneous apoptosis through stromal cell-derived factor-1.

Burger, Jan A., Tsukada, Nobuhiro, Burger, Meike, Zvaifler, Nathan J., Dell'Aquila, Marie, Kipps, Thomas J.·2000·Blood
DOI 10.1182/blood.v96.8.2655
Open this paper in CoChat — free account

Abstract

A subset of blood cells from patients with B-cell chronic lymphocytic leukemia (CLL) spontaneously differentiates in vitro into large, round, or fibroblast-like adherent cells that display stromal cell markers, namely vimentin and STRO-1. These cells also express stromal cell–derived factor-1 (SDF-1), a CXC chemokine that ordinarily is secreted by marrow stromal cells. Leukemia B cells attach to these blood-derived adherent cells, down-modulate their receptors for SDF-1 (CXCR4), and are protected from undergoing spontaneous apoptosis in vitro. Neutralizing antibodies to SDF-1 inhibit this effect. Moreover, the rapid deterioration in the survival of CLL B cells, when separated from such cells, is mitigated by exogenous SDF-1. This chemokine also results in the rapid down-modulation of CXCR4 and activation of p44/42 mitogen-activated protein-kinase (ERK 1/2) by CLL B cells in vitro. It is concluded that the blood of patients with CLL contains cells that can differentiate into adherent nurse-like cells that protect leukemia cells from undergoing spontaneous apoptosis through an SDF-1–dependent mechanism. In addition to its recently recognized role in CLL B-cell migration, SDF-1–mediated CLL B-cell activation has to be considered a new mechanism involved in the microenvironmental regulation of CLL B-cell survival.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1182/blood.v96.8.2655

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 2 references

Research works printed but not linked: 59

  1. Kipps, 1995, Chronic lymphocytic leukemia and related diseases., 1017
  2. Rai, 1999, Chronic lymphocytic leukemia., 1350
  3. Kitada, 1998, Expression of apoptosis-regulating proteins in chronic lymphocytic leukemia: correlations with in vitro and in vivo chemoresponses., Blood., 91, 3379, 10.1182/blood.V91.9.3379 · DOI
  4. McConkey, 1996, Apoptosis sensitivity in chronic lymphocytic leukemia is determined by endogenous endonuclease content and relative expression of BCL-2 and BAX., J Immunol., 156, 2624, 10.4049/jimmunol.156.7.2624 · DOI
  5. Collins, 1989, Spontaneous programmed death (apoptosis) of B-chronic lymphocytic leukaemia cells following their culture in vitro., Br J Haematol., 71, 343, 10.1111/j.1365-2141.1989.tb04290.x · DOI
  6. Lagneaux, 1998, Chronic lymphocytic leukemic B cells but not normal B cells are rescued from apoptosis by contact with normal bone marrow stromal cells., Blood., 91, 2387, 10.1182/blood.V91.7.2387 · DOI
  7. Holder, 1993, Suppression of apoptosis in normal and neoplastic human B lymphocytes by CD40 ligand is independent of Bc1–2 induction., Eur J Immunol., 23, 2368, 10.1002/eji.1830230948 · DOI
  8. Jurlander, 1998, The cellular biology of B-cell chronic lymphocytic leukemia., Crit Rev Oncol Hematol., 27, 29, 10.1016/S1040-8428(97)10008-7 · DOI
  9. Panayiotidis, 1996, Human bone marrow stromal cells prevent apoptosis and support the survival of chronic lymphocytic leukaemia cells in vitro., Br J Haematol., 92, 97, 10.1046/j.1365-2141.1996.00305.x · DOI · Paper page
  10. Han, 1984, Bone marrow infiltration patterns and their prognostic significance in chronic lymphocytic leukemia: correlations with clinical, immunologic, phenotypic, and cytogenetic data., J Clin Oncol., 2, 562, 10.1200/JCO.1984.2.6.562 · DOI
  11. Pangalis, 1984, Patterns of bone marrow involvement in chronic lymphocytic leukemia and small lymphocytic (well-differentiated) non-Hodgkin's lymphoma: its clinical significance in relation to their differential diagnosis and prognosis., Cancer., 54, 702, 10.1002/1097-0142(1984)54:4<702::AID-CNCR2820540418>3.0.CO;2-U · DOI
  12. Osmond, 1994, Apoptosis and macrophage-mediated cell deletion in the regulation of B lymphopoiesis in mouse bone marrow., Immunol Rev., 142, 209, 10.1111/j.1600-065X.1994.tb00891.x · DOI
  13. Melchers, 1995, Positive and negative selection events during B lymphopoiesis., Curr Opin Immunol., 7, 214, 10.1016/0952-7915(95)80006-9 · DOI
  14. Lu, 1999, Regulation of cell survival during B lymphopoiesis: suppressed apoptosis of pro-B cells in P53-deficient mouse bone marrow., Eur J Immunol., 29, 2484, 10.1002/(SICI)1521-4141(199908)29:08<2484::AID-IMMU2484>3.0.CO;2-B · DOI
  15. Hanada, 1993, bcl-2 gene hypomethylation and high-level expression in B-cell chronic lymphocytic leukemia., Blood., 82, 1820, 10.1182/blood.V82.6.1820.1820 · DOI
  16. Whitlock, 1982, Long-term culture of B lymphocytes and their precursors from murine bone marrow., Proc Natl Acad Sci U S A., 79, 3608, 10.1073/pnas.79.11.3608 · DOI
  17. Witte, 1987, Relationships between B-lineage lymphocytes and stromal cells in long-term bone marrow cultures., Eur J Immunol., 17, 1473, 10.1002/eji.1830171014 · DOI
  18. Burger, 1999, Chronic lymphocytic leukemia B cells express functional CXCR4 chemokine receptors that mediate spontaneous migration beneath bone marrow stromal cells., Blood., 94, 3658, 10.1182/blood.V94.11.3658 · DOI
  19. Jacobsen, 1990, Microenvironmental organization and stromal cell associations of B lymphocyte precursor cells in mouse bone marrow., Eur J Immunol., 20, 2395, 10.1002/eji.1830201106 · DOI
  20. Nagasawa, 1994, Molecular cloning and structure of a pre-B-cell growth-stimulating factor., Proc Natl Acad Sci U S A., 91, 2305, 10.1073/pnas.91.6.2305 · DOI
  21. Bleul, 1996, A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1)., J Exp Med., 184, 1101, 10.1084/jem.184.3.1101 · DOI · Paper page
  22. Zou, 1998, Function of the chemokine receptor CXCR4 in haematopoiesis and in cerebellar development., Nature., 393, 595, 10.1038/31269 · DOI
  23. Tachibana, 1998, The chemokine receptor CXCR4 is essential for vascularization of the gastrointestinal tract [see comments]., Nature., 393, 591, 10.1038/31261 · DOI
  24. Ma, 1998, Impaired B-lymphopoiesis, myelopoiesis, and derailed cerebellar neuron migration in CXCR4- and SDF-1-deficient mice., Proc Natl Acad Sci U S A., 95, 9448, 10.1073/pnas.95.16.9448 · DOI
  25. Nagasawa, 1996, Defects of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1., Nature., 382, 635, 10.1038/382635a0 · DOI
  26. Ma, 1999, The chemokine receptor CXCR4 is required for the retention of B lineage and granulocytic precursors within the bone marrow microenvironment., Immunity., 10, 463, 10.1016/S1074-7613(00)80046-1 · DOI · Paper page
  27. Kawabata, 1999, A cell-autonomous requirement for CXCR4 in long-term lymphoid and myeloid reconstitution., Proc Natl Acad Sci U S A., 96, 5663, 10.1073/pnas.96.10.5663 · DOI
  28. Nishii, 1999, Survival of human leukaemic B-cell precursors is supported by stromal cells and cytokines: association with the expression of bcl-2 protein., Br J Haematol., 105, 701, 10.1046/j.1365-2141.1999.01380.x · DOI
  29. Simmons, 1991, Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1., Blood., 78, 55, 10.1182/blood.V78.1.55.55 · DOI
  30. Anastasi, 1992, Detection of trisomy 12 in chronic lymphocytic leukemia by fluorescence in situ hybridization to interphase cells: a simple and sensitive method., Blood., 79, 1796, 10.1182/blood.V79.7.1796.1796 · DOI
  31. Hanauer, 1984, The glyceraldehyde 3 phosphate dehydrogenase gene family: structure of a human cDNA and of an X chromosome linked pseudogene: amazing complexity of the gene family in mouse., EMBO J., 3, 2627, 10.1002/j.1460-2075.1984.tb02185.x · DOI
  32. Leoni, 1998, Induction of an apoptotic program in cell-free extracts by 2-chloro-2'-deoxyadenosine 5'-triphosphate and cytochrome c., Proc Natl Acad Sci U S A., 95, 9567, 10.1073/pnas.95.16.9567 · DOI
  33. Zamzami, 1995, Reduction in mitochondrial potential constitutes an early irreversible step of programmed lymphocyte death in vivo., J Exp Med., 181, 1661, 10.1084/jem.181.5.1661 · DOI · Paper page
  34. Hunter, 1995, Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling., Cell., 80, 225, 10.1016/0092-8674(95)90405-0 · DOI
  35. Shirozu, 1995, Structure and chromosomal localization of the human stromal cell-derived factor 1 (SDF1) gene., Genomics., 28, 495, 10.1006/geno.1995.1180 · DOI
  36. Pablos, 1999, Stromal-cell derived factor is expressed by dendritic cells and endothelium in human skin., Am J Pathol., 155, 1577, 10.1016/S0002-9440(10)65474-0 · DOI · Paper page
  37. Ganju, 1998, The alpha-chemokine, stromal cell-derived factor-1-alpha, binds to the transmembrane G-protein-coupled CXCR-4 receptor and activates multiple signal transduction pathways., J Biol Chem., 273, 23169, 10.1074/jbc.273.36.23169 · DOI
  38. Dutt, 1998, Stromal cell-derived factor-1 alpha and stem cell factor/kit ligand share signaling pathways in hemopoietic progenitors: a potential mechanism for cooperative induction of chemotaxis., J Immunol., 161, 3652, 10.4049/jimmunol.161.7.3652 · DOI
  39. Wekerle, 1980, Thymic nurse cells: lymphoepithelial cell complexes in murine thymuses: morphological and serological characterization., J Exp Med., 151, 925, 10.1084/jem.151.4.925 · DOI
  40. Miyake, 1992, Requirement for VLA-4 and VLA-5 integrins in lymphoma cells binding to and migration beneath stromal cells in culture., J Cell Biol., 119, 653, 10.1083/jcb.119.3.653 · DOI
  41. Hiai, 1980, Symbiotic culture of mouse leukaemias: regulation of cell interaction by an activity of serum., Virchows Arch., 32, 261, 10.1007/BF02889031 · DOI
  42. Iwagami, 1994, Establishment and characterization of nurse cell-like clones from human skin: nurse cell-like clones can stimulate autologous mixed lymphocyte reaction., J Immunol., 153, 2927, 10.4049/jimmunol.153.7.2927 · DOI
  43. Shimaoka, 1998, Nurse-like cells from bone marrow and synovium of patients with rheumatoid arthritis promote survival and enhance function of human B cells., J Clin Invest., 102, 606, 10.1172/JCI3162 · DOI
  44. Lotz, 1994, Transforming growth factor beta as endogenous growth inhibitor of chronic lymphocytic leukemia B cells., J Exp Med., 179, 999, 10.1084/jem.179.3.999 · DOI
  45. Robledo, 1998, Associations between TGF-β1 receptors in human bone marrow stromal cells., Br J Haematol., 102, 804, 10.1046/j.1365-2141.1998.00820.x · DOI
  46. Steinman, 1997, Dendritic cells in the T-cell areas of lymphoid organs., Immunol Rev., 156, 25, 10.1111/j.1600-065X.1997.tb00956.x · DOI
  47. Manabe, 1994, Adhesion-dependent survival of normal and leukemic human B lymphoblasts on bone marrow stromal cells., Blood., 83, 758, 10.1182/blood.V83.3.758.758 · DOI
  48. McGinnes, 1991, Human B-lymphopoiesis is supported by bone marrow-derived stromal cells., Exp Hematol., 19, 294
  49. Merville, 1996, Bcl-2+ tonsillar plasma cells are rescued from apoptosis by bone marrow fibroblasts., J Exp Med., 183, 227, 10.1084/jem.183.1.227 · DOI
  50. Jones, 2000, The chemokine receptor CXCR3 is expressed in a subset of B-cell lymphomas and is a marker of B-cell chronic lymphocytic leukemia., Blood., 95, 627, 10.1182/blood.V95.2.627 · DOI
  51. Trentin, 1999, The chemokine receptor CXCR3 is expressed on malignant B cells and mediates chemotaxis., J Clin Invest., 104, 115, 10.1172/JCI7335 · DOI
  52. Bleul, 1998, B lymphocyte chemotaxis regulated in association with microanatomic localization, differentiation state, and B cell receptor engagement., J Exp Med., 187, 753, 10.1084/jem.187.5.753 · DOI
  53. Mohle, 1999, Overexpression of the chemokine receptor CXCR4 in B cell chronic lymphocytic leukemia is associated with increased functional response to stromal cell-derived factor-1 (SDF-1)., Leukemia., 13, 1954, 10.1038/sj.leu.2401602 · DOI
  54. Roberts, 1988, Heparan sulphate bound growth factors: a mechanism for stromal cell mediated haemopoiesis., Nature., 332, 376, 10.1038/332376a0 · DOI
  55. Amara, 1999, Stromal cell-derived factor-1α associates with heparan sulfates through the first beta-strand of the chemokine., J Biol Chem., 274, 23916, 10.1074/jbc.274.34.23916 · DOI
  56. Nebreda, 1999, Cell survival demands some Rsk., Science., 286, 1309, 10.1126/science.286.5443.1309 · DOI
  57. Xia, 1995, Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis., Science., 270, 1326, 10.1126/science.270.5240.1326 · DOI
  58. Bonni, 1999, Cell survival promoted by the ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms [In Process Citation]., Science., 286, 1358, 10.1126/science.286.5443.1358 · DOI
  59. Kumar, 1998, Signaling by integrin receptors., Oncogene., 17, 1365, 10.1038/sj.onc.1202172 · DOI

Cited by

Metadata

Authors
Burger, J. A., Tsukada, N., Burger, M., Zvaifler, N. J., Dell'Aquila, M., & Kipps, T. J.
Affiliations
From the Department of Medicine, Division of Hematology/Oncology, and the Division of Rheumatology, Allergy, and Immunology, University of California at San Diego; and the Department of Immunology, The Scripps Research Institute, La Jolla, CA.
Venue
Blood
Publisher
American Society of Hematology
Published
2000-10-15
Volume
96
Issue
8
Pages
2655-2663
ISSN
  • 1528-0020
  • 0006-4971
DOI
10.1182/blood.v96.8.2655
Cited by
607
References
2
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review