CoChat
Sign Up
Cited by 226

The DOI matches its Crossref record.

Distinctive features of “nurselike” cells that differentiate in the context of chronic lymphocytic leukemia.

Tsukada, Nobuhiro, Burger, Jan A., Zvaifler, Nathan J., Kipps, Thomas J.·2002·Blood
DOI 10.1182/blood.v99.3.1030
Open this paper in CoChat — free account

Abstract

A subset of blood mononuclear cells from patients with chronic lymphocytic leukemia (CLL) can differentiate in vitro into “nurselike” cells (NLCs) that can protect CLL cells from apoptosis. NLCs express cytoplasmic vimentin and stromal-derived factor 1 (SDF-1). NLCs also express CD14, as well as CD11b, CD33, CD40, CD45RO, CD68, CD80, CD86, HLA-DQ, and HLA-DR, but not CD1a, CD2, CD3, CD11c, CD19, CD45RA, CD83, CD106, or CD154. Consistent with this phenotype, NLCs failed to differentiate from blood mononuclear cells that were depleted of CD14+ cells or from isolated CD19+cells. CD14+ blood cells of healthy donors could differentiate into cells with the morphology and phenotype of NLCs when cultured in direct contact with CLL B cells, but not with normal B cells. Despite expressing antigens in common with blood monocytes, monocyte-derived dendritic cells, and macrophages, NLCs expressed significantly higher levels of CD68 than these other cell types. Consistent with the notion that NLCs are present in vivo, CD14+ splenocytes from CLL patients have NLC morphology and express significantly higher levels of CD68 than CD14+splenocytes from persons without known B-cell malignancy. These findings indicate that although NLCs may differentiate from blood monocytes, they probably represent a distinctive hematopoietic cell type that exists in vivo, differentiates from hematopoietic CD14+ cells in the context of CLL, and in turn protect CLL cells from apoptosis via a mechanism that is independent of CD106 (vascular cell adhesion molecule-1). The interaction between CLL cells and NLCs may represent a novel target for therapy of patients with this disease.

Overview

Integrity signals

Registry

Crossref · 10.1182/blood.v99.3.1030

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 36

  1. Kipps, 2001, Chronic lymphocytic leukemia and related diseases., 1163
  2. Rai, 1999, Chronic lymphocytic leukemia., 1350
  3. Kitada, 1998, Expression of apoptosis-regulating proteins in chronic lymphocytic leukemia: correlations with in vitro and in vivo chemoresponses., Blood., 91, 3379, 10.1182/blood.V91.9.3379 · DOI
  4. McConkey, 1996, Apoptosis sensitivity in chronic lymphocytic leukemia is determined by endogenous endonuclease content and relative expression of BCL-2 and BAX., J Immunol., 156, 2624, 10.4049/jimmunol.156.7.2624 · DOI
  5. Collins, 1989, Spontaneous programmed death (apoptosis) of B-chronic lymphocytic leukaemia cells following their culture in vitro., Br J Haematol., 71, 343, 10.1111/j.1365-2141.1989.tb04290.x · DOI
  6. Lagneaux, 1998, Chronic lymphocytic leukemic B cells but not normal B cells are rescued from apoptosis by contact with normal bone marrow stromal cells., Blood., 91, 2387, 10.1182/blood.V91.7.2387 · DOI
  7. Holder, 1993, Suppression of apoptosis in normal and neoplastic human B lymphocytes by CD40 ligand is independent of Bc1–2 induction., Eur J Immunol., 23, 2368, 10.1002/eji.1830230948 · DOI
  8. Jurlander, 1998, The cellular biology of B-cell chronic lymphocytic leukemia., Crit Rev Oncol Hematol., 27, 29, 10.1016/S1040-8428(97)10008-7 · DOI
  9. Burger, 2000, Blood-derived nurse-like cells protect chronic lymphocytic leukemia B cells from spontaneous apoptosis through stromal cell-derived factor-1., Blood., 96, 2655, 10.1182/blood.V96.8.2655 · DOI · Paper page
  10. Kipps, 1985, Importance of immunoglobulin isotype in human antibody-dependent, cell-mediated cytotoxicity directed by murine monoclonal antibodies., J Exp Med., 161, 1, 10.1084/jem.161.1.1 · DOI
  11. Parham, 1979, Use of a monoclonal antibody (W6/32) in structural studies of HLA-A, B, C, antigens., J Immunol., 123, 342, 10.4049/jimmunol.123.1.342 · DOI
  12. Hu, 1999, Reduction in mitochondrial membrane potential is an early event in Fas-independent CTL-mediated apoptosis., Cell Immunol., 195, 43, 10.1006/cimm.1999.1513 · DOI
  13. Simmons, 1991, Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1., Blood., 78, 55, 10.1182/blood.V78.1.55.55 · DOI
  14. Gronthos, 1994, The STRO-1+ fraction of adult human bone marrow contains the osteogenic precursors., Blood., 84, 4164, 10.1182/blood.V84.12.4164.bloodjournal84124164 · DOI
  15. Kipps, 2001, The cluster of differentiation antigens, 141
  16. Devitt, 1998, Human CD14 mediates recognition and phagocytosis of apoptotic cells., Nature., 392, 505, 10.1038/33169 · DOI
  17. Freeman, 1995, Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules., Blood., 85, 2005, 10.1182/blood.V85.8.2005.bloodjournal8582005 · DOI
  18. Strobl, 1998, Identification of CD68+lin− peripheral blood cells with dendritic precursor characteristics., J Immunol., 161, 740, 10.4049/jimmunol.161.2.740 · DOI
  19. Rasmussen, 2000, CD68+/CD83+/CD1a− dendritic cell subsets from patients with multiple myeloma are not infected with human herpesvirus 8., Med Oncol., 17, 189, 10.1007/BF02780527 · DOI
  20. Kruse, 2000, Inhibition of CD83 cell surface expression during dendritic cell maturation by interference with nuclear export of CD83 mRNA., J Exp Med., 191, 1581, 10.1084/jem.191.9.1581 · DOI
  21. Ito, 1999, A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells., J Immunol., 163, 1409, 10.4049/jimmunol.163.3.1409 · DOI
  22. Heidenreich, 1997, Regulation of human monocyte apoptosis by the CD14 molecule., J Immunol., 159, 3178, 10.4049/jimmunol.159.7.3178 · DOI
  23. Fernandez Pujol, 2000, Endothelial-like cells derived from human CD14 positive monocytes., Differentiation., 65, 287, 10.1046/j.1432-0436.2000.6550287.x · DOI
  24. Oh, 1998, Platelet-derived growth factor-B induces transformation of fibrocytes into spindle-shaped myofibroblasts in vivo., Histochem Cell Biol., 109, 349, 10.1007/s004180050235 · DOI
  25. Bevilacqua, 1993, Endothelial-leukocyte adhesion molecules., Ann Rev Immunol., 11, 767, 10.1146/annurev.iy.11.040193.004003 · DOI
  26. Vonderheide, 1994, Residues within a conserved amino acid motif of domains 1 and 4 of VCAM-1 are required for binding to VLA-4., J Cell Biol., 125, 215, 10.1083/jcb.125.1.215 · DOI
  27. Carlos, 1994, Leukocyte-endothelial adhesion molecules., Blood., 84, 2068, 10.1182/blood.V84.7.2068.2068 · DOI
  28. Jones, 1995, Crystal structure of an integrin-binding fragment of vascular cell adhesion molecule-1 at 1.8 A resolution., Nature., 373, 539, 10.1038/373539a0 · DOI
  29. Miyake, 1992, Requirement for VLA-4 and VLA-5 integrins in lymphoma cells binding to and migration beneath stromal cells in culture., J Cell Biol., 119, 653, 10.1083/jcb.119.3.653 · DOI
  30. Dittel, 1993, Regulation of human B-cell precursor adhesion to bone marrow stromal cells by cytokines that exert opposing effects on the expression of vascular cell adhesion molecule-1 (VCAM-1)., Blood., 81, 2272, 10.1182/blood.V81.9.2272.2272 · DOI
  31. Patrick, 1995, Heterotypic adherence between human B-lymphoblastic and pre-B- lymphoblastic cells and marrow stromal cells is a biphasic event: integrin very late antigen-4 alpha mediates only the early phase of the heterotypic adhesion., Blood., 85, 168, 10.1182/blood.V85.1.168.bloodjournal851168 · DOI
  32. Reparon-Schuijt, 2000, Regulation of synovial B cell survival in rheumatoid arthritis by vascular cell adhesion molecule 1 (CD106) expressed on fibroblast-like synoviocytes., Arthritis Rheum., 43, 1115, 10.1002/1529-0131(200005)43:5<1115::AID-ANR22>3.0.CO;2-A · DOI
  33. Hayashida, 2000, Rheumatoid arthritis synovial stromal cells inhibit apoptosis and up-regulate Bcl-xL expression by B cells in a CD49/CD29-CD106-dependent mechanism., J Immunol., 164, 1110, 10.4049/jimmunol.164.2.1110 · DOI
  34. Burger, 2001, Fibroblast-like synoviocytes support B-cell pseudoemperipoiesis via a stromal cell-derived factor-1- and CD106 (VCAM-1)-dependent mechanism., J Clin Invest., 107, 305, 10.1172/JCI11092 · DOI
  35. Möhle, 1999, Overexpression of the chemokine receptor CXCR4 in B cell chronic lymphocytic leukemia is associated with increased functional response to stromal cell-derived factor-1 (SDF-1)., Leukemia., 13, 1954, 10.1038/sj.leu.2401602 · DOI
  36. Damiano, 1999, Cell adhesion mediated drug resistance (CAM-DR): role of integrins and resistance to apoptosis in human myeloma cell lines., Blood., 93, 1658, 10.1182/blood.V93.5.1658 · DOI

Cited by

See all 226

Metadata

Authors
Tsukada, N., Burger, J. A., Zvaifler, N. J., & Kipps, T. J.
Affiliations
From the Department of Medicine, Division of Hematology/Oncology, and Division of Rheumatology, Allergy, and Immunology, University of California, San Diego, La Jolla, and Department of Medicine, Freiburg University, Germany.
Venue
Blood
Publisher
American Society of Hematology
Published
2002-02-01
Volume
99
Issue
3
Pages
1030-1037
ISSN
  • 1528-0020
  • 0006-4971
DOI
10.1182/blood.v99.3.1030
Cited by
226
References
0
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Distinctive features of “nurselike” cells that differentiate in the context of chronic lymphocytic leukemia. https://app.cochat.ai/papers/10.1182/blood.v99.3.1030
The paper
Tsukada, N., Burger, J. A., Zvaifler, N. J., & Kipps, T. J. (2002). Distinctive features of “nurselike” cells that differentiate in the context of chronic lymphocytic leukemia. Blood. https://doi.org/10.1182/blood.v99.3.1030