Cited by 42Immune cells in cancerPhagocytosis and Immune RegulationGenomics and Chromatin Dynamics

Macrophage functional maturation and cytokine production are impaired in C/EBPε-deficient mice

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Tavor, Sigal, Vuong, Peter T., Park, Dorothy J., Gombart, Adrian F., Cohen, Arthur H., Koeffler, H. Phillip·2002·Blood

Abstract

Members of the CCAAT/enhancer-binding protein (C/EBP) family are involved in the regulation of cellular differentiation and function of many tissues. Unlike the other members of the family, C/EBPε expression is restricted to granulocytes, macrophages, and lymphocytes. C/EBPε is highly conserved between human and rodents and is essential for terminal granulopoiesis in both species. To study the role that C/EBPε plays in macrophages, wild-type and C/EBPε–deficient (−/−) murine macrophages obtained from thioglycollate-elicited peritoneal lavages and differentiated bone marrow cells were compared. Although macrophage development occurred in both types of mice, the C/EBPε−/− cells had a lower expression of macrophage markers and a morphologic and ultrastructural appearance of immaturity. Phagocytic function, measured by calculating the percentage of internalized opsonized fluorescein isothiocyanate (FITC)–labeled yeast, was significantly impaired in the C/EBPε−/− macrophages compared with their wild-type counterparts. Furthermore, the differential expression of 26 macrophage-specific genes between wild-type and C/EBP−/− mice was analyzed. A subset of genes involved in differentiation, immune, and inflammatory responses was found down-regulated in the C/EBP−/− macrophages. Taken together, this study implicates theC/EBPε gene as an important transcription factor required for normal function and development of macrophages.

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Crossref · 10.1182/blood.v99.5.1794

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  • Multiple genes expressed in differentiated myeloid cells, including CSF2RA, ITGAM (Mac1), CEBPE and ELANE, were significantly upregulated (FDR<0.05) upon vitC treatment.

    “Further examination revealed significant upregulation (false discovery rate <0.05) of multiple genes expressed in differentiated myeloid cells, including CSF2RA,23 ITGAM (Mac1), CEBPE24 and ELANE,25 and increased expression of genes encoding cell surface markers that define mature myeloid cells (Supplementary Figure S2b, upper).”

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Research works printed but not linked: 2

  1. Kawano S, Gombart AF, Hisatake Y, et al. TCR- mediated proliferation of T-cells is severely im- paired in C/EBP epsilon knockout mice [abstract]. Blood. 1999;94:370.
  2. Fantuzzi G, Reed DA , Dinarello CA. IL12-in- duced INF-␥ is dependent on caspase-1 process- ing of the IL-18 precursor. J Clin Invest. 1999; 104:761-767.

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Authors
Tavor, S., Vuong, P. T., Park, D. J., Gombart, A. F., Cohen, A. H., & Koeffler, H. P.
Affiliations
Cedars-Sinai Medical Center (US)
Venue
Blood
Published
2002-03-01
Volume
99
Issue
5
Pages
1794-1801
ISSN
  • 0006-4971 (ISSN-L)
  • 1528-0020
OpenAlex
W1991779356
Cited by
42
References
41
Citations by year
2026: 2 · 2025: 2 · 2024: 2 · 2023: 1 · 2019: 2 · 2017: 1 · 2016: 1 · 2015: 4 · 2014: 1 · 2013: 1 · 2012: 1
FWCI
0.77
Type
journal article
Language
en
Open access
Locations
  • Blood (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
  • Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
C/EBPε, C/EBP family, macrophage differentiation, macrophage maturation, phagocytosis, cytokine production, transcription factors, granulopoiesis, bone marrow-derived macrophages, peritoneal macrophages
MeSH terms
Animals, Bone Marrow Cells, Cell Differentiation, Humans, Macrophages, Phagocytosis, Thioglycolates, Cytokines, Macrophages, Peritoneal, Mice, Knockout, Cell Culture Techniques, Gene Expression Profiling, CCAAT-Enhancer-Binding Proteins, Mice

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