Macrophage functional maturation and cytokine production are impaired in C/EBPε-deficient mice
Open accessAbstract
Members of the CCAAT/enhancer-binding protein (C/EBP) family are involved in the regulation of cellular differentiation and function of many tissues. Unlike the other members of the family, C/EBPε expression is restricted to granulocytes, macrophages, and lymphocytes. C/EBPε is highly conserved between human and rodents and is essential for terminal granulopoiesis in both species. To study the role that C/EBPε plays in macrophages, wild-type and C/EBPε–deficient (−/−) murine macrophages obtained from thioglycollate-elicited peritoneal lavages and differentiated bone marrow cells were compared. Although macrophage development occurred in both types of mice, the C/EBPε−/− cells had a lower expression of macrophage markers and a morphologic and ultrastructural appearance of immaturity. Phagocytic function, measured by calculating the percentage of internalized opsonized fluorescein isothiocyanate (FITC)–labeled yeast, was significantly impaired in the C/EBPε−/− macrophages compared with their wild-type counterparts. Furthermore, the differential expression of 26 macrophage-specific genes between wild-type and C/EBP−/− mice was analyzed. A subset of genes involved in differentiation, immune, and inflammatory responses was found down-regulated in the C/EBP−/− macrophages. Taken together, this study implicates theC/EBPε gene as an important transcription factor required for normal function and development of macrophages.
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Multiple genes expressed in differentiated myeloid cells, including CSF2RA, ITGAM (Mac1), CEBPE and ELANE, were significantly upregulated (FDR<0.05) upon vitC treatment.
“Further examination revealed significant upregulation (false discovery rate <0.05) of multiple genes expressed in differentiated myeloid cells, including CSF2RA,23 ITGAM (Mac1), CEBPE24 and ELANE,25 and increased expression of genes encoding cell surface markers that define mature myeloid cells (Supplementary Figure S2b, upper).”
From Vitamin C-induced epigenomic remodelling in IDH1 mutant acute myeloid leukaemia (2018) · cites this paper as [24]
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- Authors
- Tavor, S., Vuong, P. T., Park, D. J., Gombart, A. F., Cohen, A. H., & Koeffler, H. P.
- Affiliations
- Cedars-Sinai Medical Center (US)
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2002-03-01
- Volume
- 99
- Issue
- 5
- Pages
- 1794-1801
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W1991779356
- Cited by
- 42
- References
- 41
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2023: 1 · 2019: 2 · 2017: 1 · 2016: 1 · 2015: 4 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 0.77
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- C/EBPε, C/EBP family, macrophage differentiation, macrophage maturation, phagocytosis, cytokine production, transcription factors, granulopoiesis, bone marrow-derived macrophages, peritoneal macrophages
- MeSH terms
- Animals, Bone Marrow Cells, Cell Differentiation, Humans, Macrophages, Phagocytosis, Thioglycolates, Cytokines, Macrophages, Peritoneal, Mice, Knockout, Cell Culture Techniques, Gene Expression Profiling, CCAAT-Enhancer-Binding Proteins, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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