The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
ACE-2 expression in the small airway epithelia of smokers and COPD patients: implications for COVID-19
Open accessAbstract
Abstract Introduction Coronavirus disease 2019 (COVID-19) is a respiratory infection caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). This virus uses the angiotensin converting enzyme II (ACE-2) as the cellular entry receptor to infect the lower respiratory tract. Because individuals with chronic obstructive pulmonary disease (COPD) are at increased risk of severe COVID-19, we determined whether ACE-2 expression in the lower airways was related to COPD and cigarette smoking. Methods Using RNA-seq, we determined gene expression levels in bronchial epithelia obtained from cytologic brushings of 6 th to 8 th generation airways in individuals with and without COPD. We eternally validated these results from two additional independent cohorts, which used microarray technologies to measure gene expression levels from 6 th to 12 th generation airways. Results In the discovery cohort (n=42 participants), we found that ACE-2 expression levels were increased by 48% in the airways of COPD compared with non-COPD subjects (COPD=2.52±0.66 log2 counts per million reads (CPM) versus non-COPD= 1.70±0.51 CPM, p=7.62×10 −4 ). There was a significant inverse relationship between ACE-2 gene expression and FEV1% of predicted (r=-0.24; p=0.035). Current smoking also significantly increased ACE-2 expression levels compared with never smokers (never current smokers=2.77±0.91 CPM versus smokers=1.78±0.39 CPM, p=0.024). These findings were replicated in the two eternal cohorts. Conclusions ACE-2 expression in lower airways is increased in patients with COPD and with current smoking. These data suggest that these two subgroups are at increased risk of serious COVID-19 infection and highlight the importance of smoking cessation in reducing the risk.
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Angiotensin-converting enzyme 2 protects from severe acute lung failure
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Metadata
- Authors
- Leung, J. M., Yang, C. X., Tam, A., Shaipanich, T., Hackett, T.-L., Singhera, G. K., Dorscheid, D. R., & Sin, D. D.
- Venue
- European Respiratory Journal
- Publisher
- European Respiratory Society (ERS)
- Published
- 2020-04-08
- Volume
- 55
- Issue
- 5
- Pages
- 2000688
- ISSN
- 0903-1936 (ISSN-L)
- 1399-3003
- OpenAlex
- W3015660580
- Cited by
- 845
- References
- 6
- FWCI
- 22.79
- Type
- journal article
- Language
- en
- Open access
- hybrid
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