The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Influenza virus damages the alveolar barrier by disrupting epithelial cell tight junctions
Open accessAbstract
A major cause of respiratory failure during influenza A virus (IAV) infection is damage to the epithelial–endothelial barrier of the pulmonary alveolus. Damage to this barrier results in flooding of the alveolar lumen with proteinaceous oedema fluid, erythrocytes and inflammatory cells. To date, the exact roles of pulmonary epithelial and endothelial cells in this process remain unclear. Here, we used an in vitro co-culture model to understand how IAV damages the pulmonary epithelial–endothelial barrier. Human epithelial cells were seeded on the upper half of a transwell membrane while human endothelial cells were seeded on the lower half. These cells were then grown in co-culture and IAV was added to the upper chamber. We showed that the addition of IAV (H1N1 and H5N1 subtypes) resulted in significant barrier damage. Interestingly, we found that, while endothelial cells mounted a pro-inflammatory/pro-coagulant response to a viral infection in the adjacent epithelial cells, damage to the alveolar epithelial–endothelial barrier occurred independently of endothelial cells. Rather, barrier damage was associated with disruption of tight junctions amongst epithelial cells, and specifically with loss of tight junction protein claudin-4. Taken together, these data suggest that maintaining epithelial cell integrity is key in reducing pulmonary oedema during IAV infection.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Bevilacqua, M. P., Pober, J. S., Majeau, G. R., Fiers, W., Cotran, R. S., & Gimbrone, M. A. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.12.4533 · Cited by 1,067
Cheung, C., Poon, L., Lau, A., Luk, W., Lau, Y., Shortridge, K., Gordon, S., Guan, Y., & Peiris, J. (2002) · The Lancet · doi:10.1016/s0140-6736(02)11772-7 · Cited by 889
Cytokine regulation of tight junctions
Capaldo, C. T., & Nusrat, A. (2009) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2008.08.027 · Cited by 718
Teijaro, J. R., Walsh, K. B., Cahalan, S., Fremgen, D. M., Roberts, E., Scott, F., Martinborough, E., Peach, R., Oldstone, M. B. A., & Rosen, H. (2011) · Cell · doi:10.1016/j.cell.2011.08.015 · Cited by 688
Lung Pathology in Fatal Novel Human Influenza A (H1N1) Infection
Mauad, T., Hajjar, L. A., Callegari, G. D., da Silva, L. F. F., Schout, D., Galas, F. R. B. G., Alves, V. A. F., Malheiros, D. M. A. C., Auler, J. O. C., Ferreira, A. F., et al. (2010) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.200909-1420oc · Cited by 547
Pathogenesis of influenza-induced acute respiratory distress syndrome
Short, K. R., Kroeze, E. J. B. V., Fouchier, R. A. M., & Kuiken, T. (2014) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(13)70286-x · Cited by 538
Self-citation2009 Pandemic Influenza A (H1N1)
Shieh, W.-J., Blau, D. M., Denison, A. M., DeLeon-Carnes, M., Adem, P., Bhatnagar, J., Sumner, J., Liu, L., Patel, M., Batten, B., et al. (2010) · The American Journal of Pathology · doi:10.2353/ajpath.2010.100115 · Cited by 429
Herold, S., Steinmueller, M., von Wulffen, W., Cakarova, L., Pinto, R., Pleschka, S., Mack, M., Kuziel, W. A., Corazza, N., Brunner, T., et al. (2008) · The Journal of Experimental Medicine · doi:10.1084/jem.20080201 · Cited by 376
H5N1 infection of the respiratory tract and beyond: a molecular pathology study
Gu, J., Xie, Z., Gao, Z., Liu, J., Korteweg, C., Ye, J., Lau, L. T., Lu, J., Gao, Z., Zhang, B., et al. (2007) · The Lancet · doi:10.1016/s0140-6736(07)61515-3 · Cited by 375
Pathology of human influenza revisited
Kuiken, T., & Taubenberger, J. K. (2008) · Vaccine · doi:10.1016/j.vaccine.2008.07.025 · Cited by 360
Self-citation
Cited by
Acute respiratory distress syndrome
Matthay, M. A., Zemans, R. L., Zimmerman, G. A., Arabi, Y. M., Beitler, J. R., Mercat, A., Herridge, M., Randolph, A. G., & Calfee, C. S. (2019) · Nature Reviews Disease Primers · doi:10.1038/s41572-019-0069-0 · Cited by 2,387
The role of the microbiota in infectious diseases
Libertucci, J., & Young, V. B. (2018) · Nature Microbiology · doi:10.1038/s41564-018-0278-4 · Cited by 357
Low ambient humidity impairs barrier function and innate resistance against influenza infection
Kudo, E., Song, E., Yockey, L. J., Rakib, T., Wong, P. W., Homer, R. J., & Iwasaki, A. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1902840116 · Cited by 341
Intensive care management of influenza-associated pulmonary aspergillosis
Koehler, P., Bassetti, M., Kochanek, M., Shimabukuro-Vornhagen, A., & Cornely, O. A. (2019) · Clinical Microbiology and Infection · doi:10.1016/j.cmi.2019.04.031 · Cited by 82
Metadata
- Authors
- Short, K. R., Kasper, J., van der Aa, S., Andeweg, A. C., Zaaraoui-Boutahar, F., Goeijenbier, M., Richard, M., Herold, S., Becker, C., Scott, D. P., Limpens, R. W. A. L., Koster, A. J., Bárcena, M., Fouchier, R. A. M., Kirkpatrick, C. J., & Kuiken, T.
- Venue
- European Respiratory Journal
- Publisher
- European Respiratory Society (ERS)
- Published
- 2016-01-07
- Volume
- 47
- Issue
- 3
- Pages
- 954-966
- ISSN
- 0903-1936 (ISSN-L)
- 1399-3003
- OpenAlex
- W2291675941
- Cited by
- 257
- References
- 38
- FWCI
- 10.09
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Influenza virus damages the alveolar barrier by disrupting epithelial cell tight junctions. https://app.cochat.ai/papers/10.1183/13993003.01282-2015
- The paper
- Short, K. R., Kasper, J., van der Aa, S., Andeweg, A. C., Zaaraoui-Boutahar, F., Goeijenbier, M., Richard, M., Herold, S., Becker, C., Scott, D. P., Limpens, R. W. A. L., Koster, A. J., Bárcena, M., Fouchier, R. A. M., Kirkpatrick, C. J., & Kuiken, T. (2016). Influenza virus damages the alveolar barrier by disrupting epithelial cell tight junctions. European Respiratory Journal. https://doi.org/10.1183/13993003.01282-2015
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
