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SolexaQA: At-a-glance quality assessment of Illumina second-generation sequencing data
Open accessAbstract
BACKGROUND: Illumina's second-generation sequencing platform is playing an increasingly prominent role in modern DNA and RNA sequencing efforts. However, rapid, simple, standardized and independent measures of run quality are currently lacking, as are tools to process sequences for use in downstream applications based on read-level quality data. RESULTS: We present SolexaQA, a user-friendly software package designed to generate detailed statistics and at-a-glance graphics of sequence data quality both quickly and in an automated fashion. This package contains associated software to trim sequences dynamically using the quality scores of bases within individual reads. CONCLUSION: The SolexaQA package produces standardized outputs within minutes, thus facilitating ready comparison between flow cell lanes and machine runs, as well as providing immediate diagnostic information to guide the manipulation of sequence data for downstream analyses.
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“Furthermore, there is an exponential increase in error rates (ERs) along the reads (Cox et al., 2010).”
From PEAR: a fast and accurate Illumina Paired-End reAd mergeR (2014) · cites this paper as Cox et al., 2010
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Metadata
- Authors
- Cox, M. P., Peterson, D. A., & Biggs, P. J.
- Affiliations
- Massey University (NZ)
- Corresponding author
- Cox, Murray P
- Venue
- BMC Bioinformatics
- Published
- 2010-09-27
- Volume
- 11
- Issue
- 1
- Pages
- 485-485
- ISSN
- 1471-2105 (ISSN-L)
- PMC
- PMC2956736
- OpenAlex
- W2097205777
- Cited by
- 1,357
- References
- 9
- Citations by year
- 2026: 29 · 2025: 50 · 2024: 57 · 2023: 86 · 2022: 86 · 2021: 131 · 2020: 118 · 2019: 118 · 2018: 135 · 2017: 105 · 2016: 148 · 2015: 95 · 2014: 97 · 2013: 56 · 2012: 37
- FWCI
- 13.30
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- BMC Bioinformatics (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Royal Society
- Royal Society Te Apārangi (MAU0802)
- Massey University
- Marsden Fund (MAU0802)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fractal and DNA sequence analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genetics, Bioinformatics, and Biomedical Research
- Keywords
- sequence trimming, quality control software
- MeSH terms
- Base Sequence, Software, Sequence Analysis, DNA, Sequence Analysis, RNA, Internet
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