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Applicability of RNA standards for evaluating RT-qPCR assays and platforms
Open accessAbstract
The availability of diverse RT-qPCR assay formats and technologies hinder comparability of data between platforms. Reference standards to facilitate platform evaluation and comparability are needed. We have explored using universal RNA standards for comparing the performance of a novel qPCR platform (Fluidigm® BioMark™) against the widely used ABI 7900HT system. Our results show that such standards may form part of a toolkit to evaluate the key performance characteristics of platforms.
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Improvements to qPCR using similar technologies to those advancing dPCR will increase throughput of qPCR, at the cost of smaller input volume which often requires pre-amplification; this is not routinely needed for dPCR.
“Improvements to qPCR using similar technologies to those advancing dPCR will increase the throughput of qPCR, as has already been described (21), although this is also at the cost of smaller input volume which often requires pre-amplification (39); this is not routinely needed for dPCR.”
From Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation (2012) · cites this paper as [39]
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Metadata
- Authors
- Devonshire, A. S., Elaswarapu, R., & Foy, C. A.
- Affiliations
- LGC (GB)
- Corresponding author
- Elaswarapu, Ramnath
- Venue
- BMC Genomics
- Published
- 2011-02-18
- Volume
- 12
- Issue
- 1
- Pages
- 118-118
- ISSN
- 1471-2164 (ISSN-L)
- PMC
- PMC3052187
- OpenAlex
- W2088925963
- Cited by
- 42
- References
- 22
- Citations by year
- 2025: 1 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 3 · 2018: 3 · 2017: 4 · 2016: 3 · 2015: 5 · 2014: 5 · 2013: 4 · 2012: 6
- FWCI
- 1.95
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- BMC Genomics (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Institute of Standards and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Health Sciences › Health Professions › Medical Laboratory Technology › Clinical Laboratory Practices and Quality Control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
- Keywords
- reference materials, assay performance, data comparability
- MeSH terms
- Reference Standards, RNA, Reverse Transcriptase Polymerase Chain Reaction, Limit of Detection
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