The DOI matches its Crossref record; 72 of the 72 references it cites were found in a registry or an index.
Transcriptome sequencing and annotation of the microalgae Dunaliella tertiolecta: Pathway description and gene discovery for production of next-generation biofuels
Open accessAbstract
Background: Biodiesel or ethanol derived from lipids or starch produced by microalgae may overcome many of the sustainability challenges previously ascribed to petroleum-based fuels and first generation plant-based biofuels. The paucity of microalgae genome sequences, however, limits gene-based biofuel feedstock optimization studies. Here we describe the sequencing and de novo transcriptome assembly for the non-model microalgae species, Dunaliella tertiolecta, and identify pathways and genes of importance related to biofuel production. Results: Next generation DNA pyrosequencing technology applied to D. tertiolecta transcripts produced 1,363,336 high quality reads with an average length of 400 bases. Following quality and size trimming, ~ 45% of the high quality reads were assembled into 33,307 isotigs with a 31-fold coverage and 376,482 singletons. Assembled sequences and singletons were subjected to BLAST similarity searches and annotated with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) orthology (KO) identifiers. These analyses identified the majority of lipid and starch biosynthesis and catabolism pathways in D. tertiolecta. Conclusions: The construction of metabolic pathways involved in the biosynthesis and catabolism of fatty acids, triacylglycrols, and starch in D. tertiolecta as well as the assembled transcriptome provide a foundation for the molecular genetics and functional genomics required to direct metabolic engineering efforts that seek to enhance the quantity and character of microalgae-based biofuel feedstock.
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InfluentialHigh-throughput functional annotation and data mining with the Blast2GO suite
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Cited by
Microalgae-based carbohydrates for biofuel production
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Bioethanol production using carbohydrate-rich microalgae biomass as feedstock
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Algae biofuel: Current status and future applications
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Algae based biorefinery—How to make sense?
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Metadata
- Authors
- Rismani-Yazdi, H., Haznedaroglu, B. Z., Bibby, K., & Peccia, J.
- Venue
- BMC Genomics
- Published
- 2011-03-14
- Volume
- 12
- Issue
- 1
- Pages
- 148-148
- ISSN
- 1471-2164 (ISSN-L)
- OpenAlex
- W2009092008
- Cited by
- 296
- References
- 72
- FWCI
- 9.46
- Type
- journal article
- Language
- en
- Open access
- gold
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